An observational study in Multiple Sclerosis, Neurodegeneration and Central Nervous System Lesion, sponsored by University of Southern Denmark. Completed at 1 site in Denmark. Open to participants aged 18 Years to 65 Years. Per ClinicalTrials.gov, last updated 2021-10-14.
Sponsored by University of Southern Denmark · Observational
Among 89 studies in Multiple Sclerosis with a site in Denmark — 15 open to participants now, 24 under way, 8 of them phase 3. Multiple Sclerosis · Denmark.
Among the 89 studies in Multiple Sclerosis with a site in Denmark, this one is the 15th largest by enrollment of the 18 observational ones that give a count, is one of 6 that started in 2017, is one of 18 observational ones, and was the 50th registered.
3,267 studies on the registry are indexed under Multiple Sclerosis; 662 are open to participants now.
Browse Multiple Sclerosis studies →15 of the 89 studies in Multiple Sclerosis with a site in Denmark are open now. Recruiting first, the newest first:
Daphne Kos · Sites in Denmark, Belgium
Ages 18+
Glostrup University Hospital, Copenhagen · Sites in Denmark
Ages 18–65
University of Aarhus · Sites in Denmark
Ages 18+
Zealand University Hospital · Sites in Denmark
Ages 18–70
Novartis Pharmaceuticals · Sites in Denmark, United States, Argentina and 33 more countries
Ages 18–65
A study can be open while its sites in Denmark have not opened yet or have stopped. Only those recruiting at a site in Denmark →
Of the 430 Multiple Sclerosis studies recruiting now, 163 (38%) set no upper age limit and 211 (49%) stop at 75 or younger; 40 take children under 18; 96 take healthy volunteers.
This study: 18 Years to 65 Years · all sexes.
The full table on the hub →This tab as a report to print or save as PDF → With an account.
89 studies; the count open now beside each. This study's row is marked.
First in 2002; this study’s year is marked. 6 started in 2017.
By drug class, each counting the studies that test any of its medicines:
This study's enrollment of 49 is below the median of 100 across 971 observational studies indexed under Multiple Sclerosis.
Median enrollment 177 across the 71 interventional studies, 277 across the 18 observational. Of the 57 that completed or were terminated, 22 (39%) have results posted.
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45 of 89 have an industry lead sponsor. The 10 most frequent:
University of Southern Denmark is the lead sponsor of 336 studies on the registry; 46 are open to participants now.
University of Southern Denmark has 6 studies filed under Multiple Sclerosis — fewer than ten, so there is no comparison to draw.
7 of the studies in Multiple Sclerosis with a site in Denmark that are under way reach primary completion within a year, 2 of them phase 3. Phase 3 first, then by date:
| Study | Primary completion |
|---|---|
| NCT03477500Phase 3Haukeland University HospitalRandomized Autologous heMatopoietic Stem Cell Transplantation Versus Alemtuzumab, Cladribine or Ocrelizumab for RRMS (RAM-MS) | 2026-11-21 |
| NCT06141473Phase 3SanofiEfficacy and Safety Studies of Frexalimab (SAR441344) in Adults With Relapsing Forms of Multiple Sclerosis | 2027-05-06 |
| NCT07310914Danish Multiple Sclerosis HospitalsImplementation of an Advanced Telerehabilitation Solution for People With Multiple Sclerosis | 2026-12-31 |
| NCT06782724University Medical Center GroningenPsilocybin Therapy for Psychological Distress in Palliative Patients | 2027-01-01 |
| NCT05816122Ad scientiamMS-DETECT: Early Detection of Multiple Sclerosis Progression With MSCopilot® Detect | 2027-05-31 |
Add all 7 to your calendar (.ics) → With an account.
Among the 89 studies in Multiple Sclerosis with a site in Denmark, from their registry updates:
This tab as a report to print or save as PDF → With an account.
OBJECTIVE To investigate neurodegeneration and demyelination in the central and peripheral nervous system in multiple sclerosis linked to disease progression and mechanisms that can explain different responses to Fampridine treatment in MS patients with walking disability.
METHOD The study is a prospective cohort follow-up study with 98 participants with MS and walking disability. Participants are identified as responders or non-responders to Fampridine treatment prior to the study. Participants will undergo MRI of the cerebrum with lesion load quantification, neurophysiological tests comprised of motor evoked potentials and electroneurographic examination, blood samples examining KIR4.1 antibodies, brain derived neurotrophic factor (BDNF), myelin protein zero (MPZ), peripheral myelin protein 22 (PMP22), p75-nerve growth factor receptor (p75NGFR) and anti-myelin associated glycoprotein (anti-MAG). The presence of SORCS-3 gene mutation will also be examined, as will cerebrospinal fluid levels of myelin basic protein, neurofilament heavy and light chains. Functional test of Timed 25-foot walk test (T25FW) will identify response to Fampridine treatment. A functional test battery will further detail function of upper extremities and cognition.
CONCLUSION This study will add to the understanding of neurodegeneration and demyelination in CNS and PNS in patients with MS having walking disability. This will impact clinical decision-making by improving organization of immunomodulatory treatment, identifying biomarkers thus facilitating earlier treatment and improving patient control, information and education.
BACKGROUND Multiple Sclerosis (MS) is an autoimmune, inflammatory demyelinating disease targeting myelinated axons in the Central Nervous System (CNS). It is the most common nontraumatic cause of disability in young people. Most MS patients are diagnosed between the ages of 20 and 40 years. Denmark has a prevalence of approximately 155 MS patients per 100,000 inhabitants, which is among the highest in the world. The etiology of MS is not known, however, a combination of genetic and environmental factors is likely to be involved in triggering the disease.
CLINICAL PRESENTATION MS damages the CNS and causes progressive disability. Clinical symptoms of the patient depend on the location of the lesions that can occur anywhere in the CNS. Consequently the neurological impairments are very variable and consist of sensory, motor, visual, urinary, coordination and cognitive deficits. MS is divided into several categories based on disease progression: relapse-remitting multiple sclerosis (RRMS), secondary progressive multiple sclerosis (SPMS) and primary progressive multiple sclerosis (PPMS). Clinically isolated syndrome (CIS) is a single relapse compatible with MS accompanied by paraclinical evidence of demyelination with more than 80 percent of patients with CIS developing MS at a later stage.
WORKUP
McDonald criteria are used for diagnosing MS. They are based on clinical findings supported by paraclinical tests mentioned below. The aim is to demonstrate neurological impairments disseminated in time (lesions in the CNS are of different age) and localization in the CNS, while excluding other conditions:
Evoked Potentials are useful at identifying subclinical lesions.
OTHER ASPECTS OF CURRENT WORKUP
The other aspects of the workup that are mentioned below, are not a part of the established workup on diagnosing or monitoring MS:
Blood Samples: Currently, there are no established peripheral blood biomarkers for MS. A study has demonstrated the SORCS3-gene as a potential MS risk gene. It has not been evaluated as a peripheral blood biomarker for MS as of yet, despite being potentially important in the pathogenesis of MS.
Furthermore a study has screened serum samples aiming to identify CNS-specific antibodies in MS.
Antibodies to the glial potassium channel KIR4.1 (Inward-rectifier potassium ion channel) was present in a subgroup of MS patients while being absent among patients with other neurological conditions and healthy controls.
Brain Derived Neurotrophic Factor (BDNF) is suggested to play a neuroprotective role in MS. BDNF concentrations has been shown to be lower in patients with RRMS compared to healthy controls.
There are currently biomarkers being indicative of demyelination in the PNS. Myelin protein zero (MPZ) is a cell surface component of myelin, which decreases in the tissue when demyelination occurs in the PNS. Peripheral myelin protein 22 (PMP22) is a glycoprotein component of myelin only found in the PNS, where it accounts for 2-5% of the myelin protein content.
The P75 nerve growth factor receptor (p75 NGFR) also known as P75 neurotrophin receptor (p75NTR) or low-affinity nerve growth factor receptor (LNGFR) is a ligand, which plays a role in Schwann cell migration and myelination during development, apoptosis and axonal regeneration. Anti-myelin associated glycoprotein (Anti-MAG) is a myelin marker that is rather specific for the PNS.
Neurophysiological tests: Motor Evoked Potentials (MEP) assess the motor pathways by stimulating the precentral gyrus. It is valuable in evaluating descending motor pathways, PNS and muscles. Electroneurographic examination (ENG) assesses the nerve conduction speed in the PNS and is not usually applied for patients with MS. Few studies that have evaluated examining nerve conduction speeds in the PNS among MS patients show that deficits in the PNS may be common among MS patients.
TREATMENT
There are three aspects of MS-treatment:
FAMPRIDINE Fampridine (Ampyra/Dalfampridine/4-aminopyridine) is the first oral agent approved by the Food and Drug Administration (FDA) and European Medicines Agency (EMA) for the treatment of walking disability in MS. It was approved in 2011 in Denmark for treatment of MS patients with walking disability.
Fampridine is a potassiumchannel blocker with a primary mechanism of action of blocking voltage-gated potassium channels. It blocks potassium from entering the channel, which leads to smoother nerve conduction and a subsequent improved amplitude and duration of the action potential. Fampridine acts at both the CNS and PNS and enhances nerve conduction in demyelinated axons and improves walking ability in a subset of MS patients. Furthermore a study has demonstrated improvement in cognition and upper extremity functioning, as a result of Fampridine treatment. Patients who do not profit from Fampridine treatment, are classified as non-responders. Currently, the mechanism behind non-response to Fampridine and conversion from response to non-response has not been studied. The functional test of T25FW is widely used to discriminate patients as responders or non-responders as well as to monitor response to treatment with Fampridine. When Fampridine treatment is indicated, patients will undergo a short-term treatment period while undergoing the T25FW in order to establish if there is response to treatment or non-response. If patients are classified as the latter, treatment will be stopped. During long-term treatment with Fampridine, the initial responders are yearly evaluated as to whether they are continuous responders.
SUMMARY OF STUDY RATIONALE Walking impairment is one of the most common symptoms of MS and has been reported as one of the most impactful symptoms on quality of life. MS patients with walking impairments receive the potassium-channel blocker, Fampridine, if they respond to this treatment. It has been established that approximately 35% of MS patients with walking disabilities are responders to this treatment, and thus have improvements in their walking speed and acceleration. The response status is established using the functional test T25FW. The presence of a specific potassiumchannel antibody (KIR 4.1) has been demonstrated in a subtype of MS patients while being absent in healthy controls. Antibodies affecting the PNS and thus aggravating walking abilities have been studied, but their links to MS remain to be investigated.
MS patients undergo neurodegeneration in the CNS with progressive neurological disability associated with axonal and neuronal damage, which is a major contributor to walking impairment. The involvement of the PNS in the dysfunction of the lower extremities as well as the role of the PNS as a potential marker of disease progression in MS remains to be fully elucidated. The effect of Fampridine in relation to the PNS has also not been examined and the mechanism behind non-response to Fampridine treatment and conversion from response to non-response remains to be elucidated.
Consequently, the overall research question of the present study proposal is to further clarify disease mechanisms involved in MS. Moreover, the overall aim of this project is to expand the knowledge on 1) neurodegeneration and demyelination in the central and peripheral nervous system in MS linked to disease progression over time and 2) to examine mechanisms that can explain the different responses to Fampridine treatment. Below, the specific study aims and hypotheses are outlined.
STUDY AIMS
Overall one main study is conducted, which has three inherent sub-studies. The aims of the main study Central and Peripheral Nervous System Changes as Markers of Disease Progression in Multiple Sclerosis are therefore:
Null hypotheses are:
Participants will undergo MRI, blood samples, neurophysiologic examinations and the functional testing, as described in the trial outline section, in order to evaluate response to treatment with Fampridine and markers of disease progression. In addition to the widely used T25FW, additional functional tests will also be performed to have a more detailed overview of the function of the lower and upper extremities while also examining cognition over time. The T25FW test will also be used for identification of responders to treatment with Fampridine.
Participants in this cohort will be recruited from the four Multiple Sclerosis Centers in the Region of Southern Denmark (Sønderborg, Odense, Vejle/Kolding and Esbjerg), which (as of January 2016) have approximately 3600 patients with MS.
Participants, who are classified as responders to Fampridine treatment, have already been identified in the MS-centers in the Region of Southern Denmark, where they undergo outpatient treatment and clinical controls. A 2-week Fampridine treatment phase, as described in the trial outline section, has been used to identify responders to Fampridine. Participants who improve with ≥20% on the T25FW are categorized as responders.
Go through the criteria one by one — yes, no or not sure — and take the list to your doctor or the study team. Kept in this browser only.
Exclusion Criteria:
Underlined terms in the criteria open these too. Explained by clinicaltrials.gg, not part of the registry record. The study team decides who can take part.
GFR (glomerular filtration rate) is how much blood the kidneys filter each minute. It is usually estimated from a blood test — eGFR, by formulas such as CKD-EPI or MDRD — and some studies ask for it to be measured directly instead. Usually given in mL/min/1.73 m².
Check the method, the units and the minimum the study states. A value of 90 or above is in the normal-or-high range, but kidney damage can show in other tests too.
GFR and eGFR (kidney filtering)
GFR (glomerular filtration rate) is how much blood the kidneys filter each minute. It is usually estimated from a blood test — eGFR, by formulas such as CKD-EPI or MDRD — and some studies ask for it to be measured directly instead. Usually given in mL/min/1.73 m².
Check the method, the units and the minimum the study states. A value of 90 or above is in the normal-or-high range, but kidney damage can show in other tests too.
Explained by clinicaltrials.gg, not part of the registry record. The study team decides who can take part.
Participants, who are classified as responders to Fampridine treatment, have already been identified in the MS-centers in the Region of Southern Denmark, where they undergo outpatient treatment and clinical controls. A 2-week Fampridine treatment phase, as described in the trial outline section, has been used to identify responders to Fampridine. Participants who improve with ≥20% on the T25FW are categorized as responders.
Participants, who are classified as non-responders to Fampridine treatment, have already been identified in the MS-centers in the Region of Southern Denmark, where they undergo outpatient treatment and clinical controls. A 2-week Fampridine treatment phase, as described in the trial outline section, has been used to identify non-responders to Fampridine. Participants who do not improve with ≥20% on the T25FW are categorized as non-responders.
Timed 25-Foot Walk Test (T25FW)
The patient walks 25 feet as fast as possible. The test is repeated twice and the mean is used as the test result. This functional test measures walking speed and acceleration. This functional test has a high inter-rater and test-retest reliability and shows evidence of good concurrent validity. Hobart et al. have estimated the MCID for T25FW as an improvement \>20% and Jensen et al. have recently estimated it to 17.8% when based on data distribution approach.
Time frame: Baseline up to 6, 12 and 18 months.
Twelve Item MS Walking Scale (MSWS-12)
Questionnaire regarding assessment of walking speed from the perspective of the patient.
Time frame: Baseline up to 6, 12 and 18 months.
Guys Neurological Disability Scale (GNDS)
The Guy's Neurological Disability Scale (GNDS) is a clinical disability scale which encompass disabilities seen in multiple sclerosis. It has 12 separate categories which include cognition, mood, vision, speech, swallowing, upper limb function, lower limb function, bladder function, bowel function, sexual function, fatigue, and 'others'.
Time frame: Baseline up to 6, 12 and 18 months.
5-Times-Sit-to-Stand Test (5-STS)
The patient sits on a chair and stands up as fast as possible. This is repeated 5 times. Time stops when the patient has sat down up for the 5th time. This functional test measures strength and balance in lower extremities. The 5-STS shows validity and good test-retest and interobserver reliability. A 25.5% improvement on the 5-STS has been suggested to indicate true change and Jensen et al. have estimated the MCID to be 34.6%.
Time frame: Baseline up to 6, 12 and 18 months.
The Six Spot Step Test (SSST)
The patient walks in a crisscross pattern to six wooden markings on the floor as fast as possible while kicking each wooden disc to the side. The test is repeated twice with each leg and the mean is used as the test result. This functional test measures coordination, acceleration, speed and balancing. The re-test reliability and measurement error of the SSST is equally good as the T25FW. However, the sensitivity and discriminatory power of the SSST is better than that of the T25FW. Jensen et al. have estimated an MCID of 16.7%.
Time frame: Baseline up to 6, 12 and 18 months.
Antibodies
Blood samples will be taken examining blood levels of KIR4.1 antibodies, brain derived neurotrophic factor (BDNF), myelin protein zero (MPZ), peripheral myelin protein 22 (PMP22), p75-nerve growth factor receptor (p75NGFR) and anti-myelin associated glycoprotein (anti-MAG). The aforementioned are know to have a role in (de)myelination in the PNS.
Time frame: Baseline up to 12 months.
9-Hole Peg Test (9-HPT)
The patient undergoes this test with the dominant upper extremity and afterwards by the non-dominant upper extremity. 9 small pegs are placed into 9 holes and are removed and placed in a bowl as fast as possible. Time stops when the last peg is removed from the hole. The test is repeated twice for each upper limb and the mean is used as the test result for each upper limb. This functional test measures coordination and fine motor skills in the upper extremities. This test has high test-retest reliability. Jensen et al. have estimated an MCID of 15.3%.
Time frame: Baseline up to 6, 12 and 18 months.
Symbol Digit Modalities Test (SDMT)
The patient is presented to nine symbols, each representing a number from 0-9. A sheet containing 100 symbols should be paired with the corresponding numbers as fast as possible in 90 seconds. The amount of correct answers is the test result. This functional test measures cognition and more specifically processing speed. SDMT is sensitive for the detection of cognitive deterioration over time and is a reliable test over multiple test-retest intervals. Blum et al. have estimated the MCID for the SDMT as 5.1 arbitrary units (a.u.) and Jensen et al. have estimated an MCID as 17.1% and 6.2 a.u..
Time frame: Baseline up to 6, 12 and 18 months.
MRI of the brain
MRI with T2-weighted sequences of the brain will be used to estimate the lesion load in the CNS, which will be compared to the clinical neurologic symptoms while serving as a reference to the MRI a year after. The locations of the T2 lesions and lesion load in the CNS will be compared between responders and non-responders to Fampridine treatment.
Time frame: Baseline up to 12 months.
Neurophysiologic examinations
MEP, combined with ENG, will be performed in order to investigate nerve conduction in the motor pathways of the CNS and PNS.
Time frame: Baseline up to 12 months.
Plan to share: Undecided
Ran from Aug 2017 to primary completion in Jun 2020, 34 months.
Of 335 non-industry observational studies under Multiple Sclerosis, half reached primary completion within 36 months of starting (the median), counting those still running; a quarter within 13 and three quarters within 81. This study's 34 months is close to that.
Counting every sponsor, half of 426 studies reached it within 41 months; a quarter within 15 and three quarters within 75.
How these figures are worked out, and which studies are left out of them.
This study is completed, as verified in Oct 2021. You cannot join it, but the record below documents what was studied.
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University of Southern Denmark →