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RecruitingNCT07441733INFLUENCE IIUpdated May 11, 2026

Evaluate the Implementation of Using Indocyanine Green for Sentinel Lymph Node Mapping in Breast Cancer Patients

An interventional study of ICG used as a tracer for sentinel lymph node and technetium 99 used as a tracer for sentinel lymph node in Breast Cancer, sponsored by Casa di Cura Dott. Pederzoli. Recruiting at 1 site in Italy. Open to female participants aged 18 Years and older. Per ClinicalTrials.gov, last updated 2026-05-11.

Sponsored by Casa di Cura Dott. Pederzoli · Not applicable, Interventional, and Other

Phase
Not applicable
Study type
Interventional
Enrollment
40
Allocation
Non-randomized
Ages
18 Years and older
Sex
Female
01

Study summary

This single-institution pilot study uses a pre- and post-implementation design to evaluate the implementation of ICG fluorescent imaging for sentinel lymph node biopsy (SLNB) in an Italian hospital.

Read the detailed description

Pre-implementation: standard sentinel node procedure using 99mTc-nanocolloid, which implies 99mTc injection the day or the morning before surgery. Transition period: participating surgeons will receive on-site training, proctoring, and ongoing guidance from experienced surgeons and early adopters of the ICG method from St. Antonius Hospital, Utrecht, The Netherlands until they achieve confidence and proficiency in using ICG for SLNB. Post-implementation: 5 mg (2 ml) ICG will be injected periareolar after administration of general anesthesia and before incision. The SLN will be visualized by fluorescent imaging using a fluorescence camera.

Main study endpoints: Primary endpoint is the identification rate of SLNs achieved by 99mTc-nanocolloid or the fluorescent signal of ICG. Secondary endpoints are total number of LNs removed, detection time, total operative time, complications and (serious) adverse events, loco regional recurrence after 1 year follow-up, pre-implementation expectations regarding ICG, post-implementation experiences regarding ICG including barriers and success factors.

02

Conditions studied

  • Breast Cancer

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Keywords

  • breast cancer
  • lymph node
  • 99mTc
  • ICG
03

Who can participate

Ages eligible
18 Years and older
Sexes eligible
Female
Accepts healthy volunteers
No

Inclusion criteria

  • Clinically node-negative, DCIS or invasive stage T1-T3 breast cancer confirmed by biopsy
  • Preoperative axillary ultrasound to confirm clinical node-negative status
  • Indication for breast cancer surgery with SLNB via axillar incision
  • Written informed consent according to national regulations

Exclusion criteria

Exclusion Criteria:

  • Patients \< 18 years old.
  • Indication for breast cancer surgery with SLNB via mastectomy incision
  • Known allergy for ICG, intravenous contrast or iodine
  • History of axillary lymph node dissection
  • Pregnancy or breast-feeding
  • Psychological, familial, sociological or geographical factors that could potentially hamper compliance with the study protocol
04

Study design

Phase
Not applicable
Primary purpose
Other
Allocation
Non-randomized
Intervention model
Parallel assignment
Masking
None (open label)
Enrollment
40 participants (estimated)

Study arms

  • Active comparator
    99mTc

    radioisotope technetium technique will be used for sentinel lymph node localization(99mTc)

    Procedure: technetium 99 used as a tracer for sentinel lymph node

  • Experimental
    ICG

    indocyanine green fluorescent imaging technique will be used for sentinel lymph node localization (ICG)

    Procedure: ICG used as a tracer for sentinel lymph node

Interventions

  • ProcedureICG used as a tracer for sentinel lymph node

    ICG used as a tracer for sentinel lymph node instead of technetium 99

  • Proceduretechnetium 99 used as a tracer for sentinel lymph node

    technetium 99 used as a tracer for sentinel lymph node instead of ICG

05

What researchers measure

Primary outcomes

  1. Identification rate of SLNs achieved by 99mTc-nanocolloid or the fluorescent signal of ICG

    measure the percentage of sentinel lymph nodes identified by the 99mTc nanocolloid or the ICG fluorescent signal (%)

    Time frame: through study completion, an average of 1 year

Secondary outcomes

  1. Percentage of SLNs that has 99mTc uptake/ is fluorescent

    Percentage of sentinel lymph nodes that have an absorption of 99mTc and are fluorescent (%)

    Time frame: through study completion, an average of 1 year

  2. Detection time

    Detection time is defined as time between skin incision and SLN resection in minutes (min)

    Time frame: through study completion, an average of 1 year

  3. Total operative time

    Registration of the duration of the total surgical procedure including the SN-procedure (min or h)

    Time frame: through study completion, an average of 1 year

  4. Number of complications and (serious) adverse events

    registration of the number of serious adverse events and complications, including seroma, wound infection and bleeding

    Time frame: through study completion, an average of 1 year

  5. loco regional recurrence after 1 year follow-up

    number of regional loco recurrences assessed through clinical examinations

    Time frame: through study completion, an average of 1 year

  6. The pathology of SLNs identified

    number of micro and macro metastases and isolated tumor cells (ITCs)

    Time frame: through study completion, an average of 1 year

06

Study locations

1 of 1 sites recruiting
  • Ospedale P. Pederzoli Casa di Cura Privata S.p.A.
    Peschiera del Garda, Verona, Italy
    Recruiting
07

References and documents

Individual participant data

Plan to share: No

No publications or documents are linked to this record.

08

Registry details

Key details

Study ID
NCT07441733
Lead sponsor
Casa di Cura Dott. Pederzoli
Responsible party
Sponsor
First posted
Mar 2, 2026
Start date
Nov 7, 2024
Primary completion
Mar 31, 2025
Completion
Oct 2026 (estimated)
Last update
May 11, 2026

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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