An interventional study of periodontal examination's, determination of salivary antioxidants and cytokine levels (VEGF, TGF-β, Il-17) in gingival crevicular fluid, Non-surgical therapy of periodontitis and Activity of antioxidants in unstimulated saliva (GPX, SOD, UA) in Systemic Sclerosis and Periodontitis, sponsored by University of Belgrade. Recruiting at 1 site in Serbia. Per ClinicalTrials.gov, last updated 2024-09-19.
Sponsored by University of Belgrade · Not applicable, Interventional, and Treatment
The aim of the research will be to evaluate the effects of NSPT on clinical periodontal parameters, level of antioxidants in unstimulated saliva and the levels of biomarkers (VEGF, TGF-β, IL-17) in the GCF in SSc patients in comparison with systemically healthy controls. Twenty systemic sclerosis patients with periodontitis (SS group) and twenty systemically healthy periodontitis patients (P group) will be enrolled in the present study. The results may indicate higher periodontal destruction and antioxidant perturbations in unstimulated saliva of systemic sclerosis patients with periodontitis compared to systemically healthy periodontitis patients.
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Exclusion Criteria:
Patients with established diagnoses of systemic sclerosis and periodontitis
Procedure: periodontal examination's, determination of salivary antioxidants and cytokine levels (VEGF, TGF-β, Il-17) in gingival crevicular fluid, Non-surgical therapy of periodontitis · Diagnostic Test: Activity of antioxidants in unstimulated saliva (GPX, SOD, UA) · Diagnostic Test: Total cytokine levels (VEGF, TGF-β, Il-17) in gingival crevicular fluid · Procedure: Periodontal treatment
Systemically healthy periodontitis patients
Procedure: periodontal examination's, determination of salivary antioxidants and cytokine levels (VEGF, TGF-β, Il-17) in gingival crevicular fluid, Non-surgical therapy of periodontitis · Diagnostic Test: Activity of antioxidants in unstimulated saliva (GPX, SOD, UA) · Diagnostic Test: Total cytokine levels (VEGF, TGF-β, Il-17) in gingival crevicular fluid · Procedure: Periodontal treatment
Periodontal parameters has been recorded on six sites of each tooth using a manual periodontal probe. Activity of antioxidants in unstimulated saliva (GPX, SOD, UA) has been determined using the colorimetric method and commercial kits. Total cytokine levels (VEGF, TGF-β, Il-17) in gingival crevicular fluid were determined using commercial ELISA (enzyme-linked immunosorbent assay) kits . The therapeutic procedure begun with ultrasonic scaling (MiniPiezon, EMS Electro Medical Systems, Switzerland), along with education and motivation of subjects to maintain oral hygiene properly. Periodontal debridement was carried out under local anesthesia, by quadrants, starting from the upper right quadrant at 7-day intervals until the completion of therapy. Instrumentation of all periodontal pockets was processed manually using specialized curettes.
Activity of antioxidants in unstimulated saliva (GPX, SOD, UA) has been determined using the colorimetric method and commercial kits.
Total cytokine levels (VEGF, TGF-β, Il-17) in gingival crevicular fluid were determined using commercial ELISA (enzyme-linked immunosorbent assay) kits .
The therapeutic procedure begun with ultrasonic scaling (MiniPiezon, EMS Electro Medical Systems, Switzerland), along with education and motivation of subjects to maintain oral hygiene properly. Periodontal debridement was carried out under local anesthesia, by quadrants, starting from the upper right quadrant at 7-day intervals until the completion of therapy. Instrumentation of all periodontal pockets was processed manually using specialized curettes.
Clinical attachment level (CAL)
Measuring the CAL by periodontal probe, measured in mm
Time frame: one day
Probing depth (PD)
Measuring the PD by periodontal probe, measured in mm
Time frame: one day
Gingival margin level (GML)
Measuring the GML by periodontal probe, measured in mm
Time frame: one day
The degree of gingival inflammation
The degree of gingival inflammation was assessed using the Loe and Silness (LSGI) gingival index.
Time frame: one day
plaque index (PI)
Evaluation of oral hygiene status was recorded with a Silness Loe plaque index (PI).
Time frame: one day
The concentration of vascular endothelial growth factor (VEGF)
The level of VEGF were determined using commercial ELISA (enzyme-linked immunosorbent assay) kits.
Time frame: baseline and two months after periodontal treatment
The concentration of transforming growth factor beta (TGF-β)
The level of TGF-β in gingival crevicular fluid was determined using commercial ELISA (enzyme-linked immunosorbent assay) kits at the Implant Research Center, School of Dental Medicine, University of Belgrade, following the manufacturer\'s instructions.
Time frame: baseline and two months after periodontal treatment
The concentration of interleukin (IL)-17
interleukin (IL)-17 was determined using commercial ELISA (enzyme-linked immunosorbent assay) kits at the Implant Research Center, School of Dental Medicine, University of Belgrade, following the manufacturer\'s instructions.
Time frame: baseline and two months after periodontal treatment
The concentration of glutathion peroxidase (GPX)
Activity level of GPX, determined using the colorimetric method and commercial kits.
Time frame: one day
The concentration of uric acid (UA)
Activity level of UA, determined using the colorimetric method and commercial kits.
Time frame: one day
The concentration of superoxide dismutase (SOD)
Activity level of SOD, determined using the colorimetric method and commercial kits.
Time frame: one day
The concentration of tumor necrosis factor alpha
Time frame: baseline and two months after periodontal treatment
The concentration of C reactive protein
Time frame: baseline and two months after periodontal treatment
Erythrocyte sedimentation rate (ESR)
Time frame: baseline and two months after periodontal treatment
Plan to share: No
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