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CompletedNCT06000410Updated Apr 21, 2026

A Study to Evaluate the Efficacy of Amniotic Suspension Allograft in Patients With Osteoarthritis of the Knee

A Phase 3 interventional study of Amniotic Suspension Allograft and Placebo in Knee Osteoarthritis, sponsored by Organogenesis. Completed at 41 sites in United States. Open to participants aged 18 Years and older. Per ClinicalTrials.gov, last updated 2026-04-21.

Sponsored by Organogenesis · Phase 3, Interventional, and Treatment

Phase
Phase 3
Study type
Interventional
Enrollment
594
Allocation
Randomized
Ages
18 Years and older
Sex
All
01

Study summary

This study is being conducted to evaluate the efficacy and safety of ASA compared to placebo in the management of osteoarthritis (OA) symptoms of the knee.

Read the detailed description

This is a prospective, multicenter, randomized, double-blind, placebo-controlled Phase 3 study of ASA in patients with OA of the knee. Initially, 474 subjects are planned for inclusion in this study using a group sequential design with an interim analyses and a final analysis. Patients will be randomly assigned in a 1:1 ratio to receive a single intra-articular (IA) injection of 2 mL of ASA (plus 2 mL of normal saline) or 4 mL of normal saline on Day 1.

They will have serial assessments of knee pain, function, and symptoms scores, as well as safety assessments for up to 52 weeks after administration of the study drug.

02

Conditions studied

  • Knee Osteoarthritis

Keywords

  • Osteoarthritis
  • Knee
  • Arthritis
  • Joint Disease
  • Musculoskeletal Diseases
  • Rheumatic Diseases
03

Who can participate

Ages eligible
18 Years and older
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  • Males or females 18 years of age or older
  • Diagnosis of OA of the index knee by a combination of clinical and radiographic findings.
  • OA of the index knee with Kellgren and Lawrence radiographic classification (Grades 2-4 inclusive),
  • Patients who have failed to adequately respond for at least 6 months to at least two osteoarthritis therapies within the last 12 months
  • Overall index knee pain score above minimum required WOMAC Pain scale.
  • Body mass index \< 40 kg/m2
  • Using birth control, sterile or post-menopausal.
  • Able to understand and provide written informed consent

Exclusion criteria

Exclusion Criteria:

  • Kellgren and Lawrence radiographic grade 1 OA of the knee
  • Use of pain medication less than 5 days before the baseline visit
  • Regular use of anticoagulants
  • Symptoms that could indicate meniscal displacement or an IA loose body.
  • Corticosteroid injection into the index knee within 3 months prior to screening.
  • Viscosupplement or any other autologous or allogeneic product into the index knee within 6 months prior to screening.
  • Patients with known hypersensitivity reactions to ASA or any of its constituents.
  • Knee surgery on the index knee within 12 months prior to screening and/or planned knee surgery during the study
  • Knee surgery on the contralateral knee within 6 months prior to screening and/or planned knee surgery during the study
  • Acute index knee trauma within 3 months prior to screening
  • Knee effusion requiring aspiration of the index or contralateral knee within 3 months prior to screening.
  • Contralateral knee pain above limits defined in the protocol
  • Current therapy with any immunosuppressive therapy or medical conditions likely to require systemic steroids during the study.
  • Any active or systemic infection including infection of the index knee joint or breakdown or disease of the index knee skin/soft tissues.
  • Clinically significant intercurrent illness, medical condition, non-knee pain, or medical history that would jeopardize patient safety, limit participation, or compromise interpretation of data derived from the patient
  • Active alcohol or substance use disorder, or any other reason that would make it unlikely that the patient will comply with study procedures
  • Females who are pregnant or lactating
  • Participation in another clinical trial within the 30 days (or 5 half-lives of the investigational compound, whichever is longer) before screening.
04

Study design

Phase
Phase 3
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Quadruple (Participant, Care provider, Investigator, Outcomes assessor)
Enrollment
594 participants (actual)

Study arms

  • Experimental
    ASA

    Participants receive a single IA injection of 2 mL of ASA (plus 2 mL of normal saline)

    Biological: Amniotic Suspension Allograft

  • Placebo comparator
    Placebo

    Participants receive a single IA injection of 4 mL of normal saline

    Drug: Placebo

Interventions

  • BiologicalAmniotic Suspension Allograft

    This investigational product is a cryopreserved product derived from human amnion and cells from the amniotic fluid.

  • DrugPlacebo

    Matching placebo is 0.9% normal saline: 4 mL to be injected IA.

05

What researchers measure

Primary outcomes

  1. The difference in change from baseline in WOMAC Pain scale at 6 months between ASA- and placebo-treated patients

    The WOMAC is widely used in the evaluation of hip and knee OA. It is a self-administered questionnaire consisting of 24 items divided into 3 subscales: pain, stiffness, and physical function. The WOMAC measure has been used in clinical trials to evaluate the efficacy of surgical treatments, medicinal and biological products, devices, and physical therapies.

    Time frame: Baseline to Week 26

Secondary outcomes

  1. The difference between changes from baseline for ASA- and placebo- treated patients in WOMAC Function at 6 months

    The WOMAC is widely used in the evaluation of hip and knee OA. It is a self-administered questionnaire consisting of 24 items divided into 3 subscales: pain, stiffness, and physical function. The WOMAC measure has been used in clinical trials to evaluate the efficacy of surgical treatments, medicinal and biological products, devices, and physical therapies.

    Time frame: Baseline to Week 26

  2. The difference between changes from baseline for ASA- and placebo-treated patients in the OMERACT-OARSI responder rate at 6 months

    The OMERACT-OARSI core domain set for clinical trials in hip and/or knee osteoarthritis responder criteria will be used to assess whether a patient is a responder or not.

    Time frame: Baseline to Week 26

  3. The difference between changes from baseline for ASA- and placebo-treated patients in WOMAC Pain at 3 months

    The WOMAC is widely used in the evaluation of hip and knee OA. It is a self-administered questionnaire consisting of 24 items divided into 3 subscales: pain, stiffness, and physical function. The WOMAC measure has been used in clinical trials to evaluate the efficacy of surgical treatments, medicinal and biological products, devices, and physical therapies.

    Time frame: Baseline to Week 12

06

Study locations

41 sites
  • Central Research Associates, Inc.
    Birmingham, Alabama 35205, United States
  • Alabama Clinical Therapeutics, LLC
    Birmingham, Alabama 35235, United States
  • Fiel Family & Sports Medicine
    Tempe, Arizona 85283, United States
  • Horizon Clinical Research
    La Mesa, California 91942, United States
  • Neurovations Research
    Napa, California 94558, United States
  • Infinity Clinical Research
    Norco, California 92860, United States
  • Dream Team Clinical Research
    Pomona, California 91767, United States
  • Stanford Medicine
    Redwood City, California 94063, United States
  • TriWest Research Associates
    San Diego, California 92108, United States
  • AppleMed Research Group, LLC.
    Miami, Florida 33126, United States
  • Gulfcoast Research Institute
    Sarasota, Florida 34232, United States
  • CenExcel/ACMR
    Atlanta, Georgia 30331, United States
  • Vista Clinical Research
    Newnan, Georgia 30265, United States
  • Chicago Clinical Research Institute Inc.
    Chicago, Illinois 60607, United States
  • Rush University Medical Center
    Chicago, Illinois 60612, United States
  • Advanced Quality Medical Research, Inc
    Orland Park, Illinois 60462, United States
  • MediSphere Medical Research Center
    Evansville, Indiana 47714, United States
  • Sinai Hospital
    Baltimore, Maryland 21215, United States
  • Klein & Associates M.D., P.A.
    Hagerstown, Maryland 21740, United States
  • Skylight Health Research
    Burlington, Massachusetts 01803, United States
  • Oakland Medical Research
    Troy, Michigan 48085, United States
  • Sundance Clinical Research
    St Louis, Missouri 63141, United States
  • Physician Research Collaboriation, LLC
    Lincoln, Nebraska 68516, United States
  • Quality Clinical Research
    Omaha, Nebraska 68114, United States
  • NYU Langone Health
    New York, New York 10016, United States
  • Hospital for Special Surgery
    New York, New York 10021, United States
  • Northwell Health
    New York, New York 10065, United States
  • Upstate Clinical Research Associates, LLC
    Williamsville, New York 14221, United States
  • West Clinical Research
    Morehead City, North Carolina 28557, United States
  • The Ohio State University Wexner Medical Center
    Columbus, Ohio 43202, United States
  • Conrad Clinical Research
    Edmond, Oklahoma 73013, United States
  • Tekton Research
    Yukon, Oklahoma 73099, United States
  • Lehigh Center for Clinical Research
    Allentown, Pennsylvania 18104, United States
  • Clinical Trials of South Carolina
    Charleston, South Carolina 29406, United States
  • Tekton Research
    Austin, Texas 78745, United States
  • Cedar Health Research, LLC
    Burleson, Texas 76028, United States
  • ClinRXResearch INC
    Carrollton, Texas 75007, United States
  • Zenos Clinical Research
    Dallas, Texas 75230, United States
  • JBR Clinical Research
    Salt Lake City, Utah 84107, United States
  • Spectrum Medical, Inc.
    Danville, Virginia 24541, United States
  • Spokane Joint Replacement Center
    Spokane, Washington 99218, United States
07

References and documents

Individual participant data

Plan to share: No

No publications or documents are linked to this record.

08

Registry details

Key details

Study ID
NCT06000410
Lead sponsor
Organogenesis
Responsible party
Sponsor
First posted
Aug 21, 2023
Start date
Sep 6, 2023
Primary completion
Aug 15, 2025
Completion
Nov 30, 2025
Last update
Apr 21, 2026

Oversight

Data monitoring committee
Yes
FDA-regulated drug
Yes
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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