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CompletedNCT04537494PREVENTUpdated May 18, 2025

PRobiotics for EVEry Newborn Trial

An interventional study of L. reuteri in Colic and Infantile, sponsored by The Hospital for Sick Children. Completed at 1 site in Canada. Open to participants aged 1 Day to 7 Days, including healthy volunteers. Per ClinicalTrials.gov, last updated 2025-05-18.

Sponsored by The Hospital for Sick Children · Not applicable, Interventional, and Prevention

Phase
Not applicable
Study type
Interventional
Enrollment
32
Allocation
Randomized
Ages
1 Day to 7 Days
Sex
All
01

Study summary

The aim of the study is to compare prevention (oral supplementation with the probiotic L. reuteri administered to every newborn within the first week of life for 12 weeks) with treatment-as-needed (supplementation with the probiotic L. reuteri after randomization, to infants who develop excessive cry/fuss up to 12 weeks of age). This is a single site pilot study to assess feasibility for a full trial.

Read the detailed description

One in five infants experience colic, defined as recurrent and prolonged episodes of crying and fussing with no obvious cause in healthy infants less than 5 months of age. There is evidence to support the role of the probiotic L. reuteri for treatment of colic in breastfed babies and for prevention of colic. However, these two options (prevention vs treatment-as-needed) have not been previously compared head-to-head. The study aims determine if oral supplementation with the probiotic L. reuteri administered to every newborn within the first week of life for 12 weeks (prevention) is superior to treatment-as-needed, as measured by daily cry/fuss duration at 6 and 12 weeks of age.

02

Conditions studied

  • Colic
  • Infantile

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Keywords

  • Colic
  • Probiotic
  • Infant
03

Who can participate

Ages eligible
1 Day to 7 Days
Sexes eligible
All
Accepts healthy volunteers
Yes

Inclusion criteria

  1. 1 to 7 days of age
  2. term (37 to 41 weeks)
  3. breast or formula fed
  4. birth weight > 2500 grams
  5. parental consent

Exclusion criteria

Exclusion Criteria:

  1. congenital or other medical disorders
  2. parents unable to communicate in English or French
04

Study design

Phase
Not applicable
Primary purpose
Prevention
Allocation
Randomized
Intervention model
Parallel assignment
Masking
None (open label)
Enrollment
32 participants (actual)

Study arms

  • Active comparator
    Prevention (Randomized)

    Oral supplementation with the probiotic L. reuteri administered to every newborn within the first week of life for 12 weeks

    Dietary Supplement: L. reuteri

  • Other
    Treatment-as-needed (Randomized)

    Supplementation with the probiotic L. reuteri after randomization, to infants who develop excessive cry/fuss up to 12 weeks of age

    Dietary Supplement: L. reuteri

  • Active comparator
    Prevention (Parent Preference)

    Oral supplementation with the probiotic L. reuteri administered to every newborn within the first week of life for 12 weeks

    Dietary Supplement: L. reuteri

  • Other
    Treatment-as-needed (Parent Preference)

    Supplementation with the probiotic L. reuteri after randomization, to infants who develop excessive cry/fuss up to 12 weeks of age

    Dietary Supplement: L. reuteri

Interventions

  • Dietary supplementL. reuteri

    Probiotic

    Also known as: BioGaia

05

What researchers measure

Primary outcomes

  1. Combined infant daily cry/fuss duration

    The primary outcome will be infant daily cry/fuss duration (combined cry and fuss in minutes) measured using the modified Baby's Day Diary over a 48 hour period.

    Time frame: Baseline

  2. Combined infant daily cry/fuss duration

    The primary outcome will be infant daily cry/fuss duration (combined cry and fuss in minutes) measured using the modified Baby's Day Diary over a 48 hour period.

    Time frame: 6 weeks

  3. Combined infant daily cry/fuss duration

    The primary outcome will be infant daily cry/fuss duration (combined cry and fuss in minutes) measured using the modified Baby's Day Diary over a 48 hour period.

    Time frame: 12 weeks

Secondary outcomes

  1. Infant daily cry duration

    Daily cry duration will be examined separately

    Time frame: Baseline

  2. Infant daily cry duration

    Daily cry duration will be examined separately

    Time frame: 6 weeks

  3. Infant daily cry duration

    Daily cry duration will be examined separately

    Time frame: 12 weeks

  4. Infant daily fuss duration

    Daily fuss duration will be examined separately

    Time frame: Baseline, 6 and 12 weeks of age

  5. Infant daily fuss duration

    Daily fuss duration will be examined separately

    Time frame: Baseline

  6. Infant daily fuss duration

    Daily fuss duration will be examined separately

    Time frame: 6 weeks

  7. Infant colic

    Daily cry/fuss of at least 180 minutes

    Time frame: 12 weeks

  8. Infant daily sleep duration

    Sleep duration will be measured objectively using actigraphy worn around the infant's ankle over a sock.

    Time frame: Baseline

  9. Infant daily sleep duration

    Sleep duration will be measured objectively using actigraphy worn around the infant's ankle over a sock.

    Time frame: 6 weeks

  10. Infant daily sleep duration

    Sleep duration will be measured objectively using actigraphy worn around the infant's ankle over a sock.

    Time frame: 12 weeks

  11. Parent (female and male) mental health

    Mental health will be measured using the Edinburgh Postnatal Depression Scale, a validated 10-item screening questionnaire. Scores range between 0 and 30 and higher scores mean worse outcome.

    Time frame: Baseline

  12. Parent (female and male) mental health

    Mental health will be measured using the Edinburgh Postnatal Depression Scale, a validated 10-item screening questionnaire. Scores range between 0 and 30 and higher score means worse outcome.

    Time frame: 6 weeks

  13. Parent (female and male) mental health

    Mental health will be measured using the Edinburgh Postnatal Depression Scale, a validated 10-item screening questionnaire. Scoring is between o and 30 and higher score means worse outcome.

    Time frame: 12 weeks

  14. Parent (female and male) fatigue

    Fatigue will be measured using a validated Fatigue Visual Analogue Scale. Scores will range between 0 and 180, higher score means worse outcome

    Time frame: Baseline

  15. Parent (female and male) fatigue

    Fatigue will be measured using a validated Fatigue Visual Analogue Scale. Scores will range between 0 and 180, higher score means worse outcome.

    Time frame: 6 weeks

  16. Parent (female and male) fatigue

    Fatigue will be measured using a validated Fatigue Visual Analogue Scale. Scores will range between 0 and 180, higher score means worse outcome

    Time frame: 12 weeks

  17. Gut microbial composition, diversity and function

    Microbial composition, diversity and function will be measured in infant fecal samples.

    Time frame: Baseline

  18. Gut microbial composition, diversity and function

    Microbial composition, diversity and function will be measured in infant fecal samples.

    Time frame: 6 weeks

  19. Gut microbial composition, diversity and function

    Microbial composition, diversity and function will be measured in infant fecal samples.

    Time frame: 12 weeks

  20. Adverse effects - digestive upset

    Number of participants with occurrences of digestive upset (e.g., diarrhea), in the Baby's Day Diary

    Time frame: 8 weeks

  21. Adverse effects - digestive upset

    Number of participants with occurrences of digestive upset (e.g., diarrhea), in the Baby's Day Diary

    Time frame: 16 weeks

  22. Adverse effects - growth/length

    Infant growth (length) will be measured at scheduled health supervision visits

    Time frame: 8 weeks

  23. Adverse effects - growth/weight

    Infant growth (weight) will be measured at scheduled health supervision visits

    Time frame: 8 weeks

  24. Adverse effects - growth/head circumference

    Infant growth (head circumference) will be measured at scheduled health supervision visits

    Time frame: 8 weeks

  25. Adverse effects - growth/length

    Infant growth (length) will be measured at scheduled health supervision visits

    Time frame: 16 weeks

  26. Adverse effects - growth/weight

    Infant growth (weight) will be measured at scheduled health supervision visits

    Time frame: 16 weeks

  27. Adverse effects - growth/head circumference

    Infant growth (head circumference) will be measured at scheduled health supervision visits

    Time frame: 16 weeks

  28. Health services utilization

    Frequency of assessments

    Time frame: Baseline

  29. Health services utilization

    Frequency of assessments

    Time frame: 6 weeks

  30. Health services utilization

    Frequency of assessments

    Time frame: 12 weeks

06

Study locations

1 site
  • The Hospital for Sick Children
    Toronto, Ontario M5G 1X8, Canada
07

Registry details

Key details

Study ID
NCT04537494
Lead sponsor
The Hospital for Sick Children
Responsible party
Patricia Parkin (Professor, Senior Associate Scientist, Staff Pediatrician, The Hospital for Sick Children) — Principal investigator
First posted
Sep 3, 2020
Start date
Dec 1, 2021
Primary completion
Sep 8, 2023
Completion
Sep 8, 2023
Last update
May 18, 2025

Study contacts

Patricia Parkin, MD
principal investigator · The Hospital for Sick Children
Patricia Li, MD
principal investigator · The Research Institute of the McGill University Health Centre

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
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