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RecruitingNCT05512234Updated Sep 22, 2025

Effect of Probiotic Limosilactobacillus Reuteri (L. Reuteri) on Crying Time in Infants With Colic

An interventional study of L. reuteri and Placebo in Colic, Infantile, sponsored by BioGaia AB. Recruiting at 2 sites in 2 countries. Open to participants aged 3 Weeks to 8 Weeks. Per ClinicalTrials.gov, last updated 2025-09-22.

Sponsored by BioGaia AB · Not applicable, Interventional, and Treatment

Phase
Not applicable
Study type
Interventional
Enrollment
102
Allocation
Randomized
Ages
3 Weeks to 8 Weeks
Sex
All
01

Study summary

This is a double-blind, multi-center, randomized, placebo-controlled, parallel-group study in infants with colic with the primary objective to evaluate crying time.

Read the detailed description

Prevalence of infantile colic varies according to the definition used but estimates range between 5 % and 26 % of the infant population. To differentiate colic from other, more serious conditions it should be characterized by several clinical features. Infant colic is often accompanied by flushing the face, frown, tensing of the abdomen, clenching of the fists, and drawing up the leg, frequent, prolonged, and intense crying or fussiness in a healthy infant. Even though infant colic is benign and usually self-limiting condition it is a source of major distress for the infant, parents, family, and health care givers.

Despite infant colic occurs frequently, little agreement has been reached on the definition, pathogenesis, or the optimal management strategy for infant colic. Recent systematic reviews and meta-analyses showed that probiotic L. reuteri DSM 17938 supplementation significantly lowered the number of hours of crying and fussing daily. A significant peak effect was seen at 3 weeks in most studies. Interestingly, babies with infant colic were found to have increased evidence of gut inflammation, as evidenced by high levels of the antimicrobial peptide fecal calprotectin; also, the levels of fecal calprotectin declined significantly as the condition resolved.

02

Conditions studied

  • Colic, Infantile

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Keywords

  • Colic
  • L. reuteri
03

Who can participate

Ages eligible
3 Weeks to 8 Weeks
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  1. Aged between 3-8 weeks at screening.
  2. Gestational age 37+0 weeks - 42+0 weeks at birth.
  3. Birth weight appropriate for gestational age (AGA - weight between 10th and 90th percentile) or Large for Gestational Age (LGA - weight above the 90th percentile) determined using WHO Weight-for Age percentile guides.
  4. Parents/caregivers/legal guardians are >18 years.
  5. Exclusively or predominantly breastfed infants (> 50 % breast fed).
  6. Willing to maintain current feeding patterns (not change formula/not change ratio of formula:breast milk etc.).
  7. Readiness and the opportunity for parents/caregivers/legal guardians to fill out a study diary, questionnaires.
  8. Infantile colic diagnosed according to Rome IV criteria (face-to-face consult with a physician, parents/caregivers/legal guardians must report that their infant has cried or fussed for 3 or more hours per day, during 3 or more days in the preceding week). At Visit 2, this will be confirmed by Baby's Day Diary®, at least one 24-hour period should show 3 or more hours of crying/fussing time.
  9. Parents/caregivers/legal guardians with ability to understand and comply with the requirements of the study, as judged by the Investigator.
  10. Parents/caregivers/legal guardians willing and able to give informed consent for their and their infant's participation in the study.
  11. The mother of the infant must be willing to attend Visit 2 (Day 0), Visit 4 (Day 8), and Visit 6 (Day 22).
  12. Infant is considered healthy, in the opinion of the investigator following physical exam.

Exclusion criteria

Exclusion criteria

  1. Infants with severe gastroesophageal reflux (throwing up or spitting up more than a teaspoon of milk > 8 times daily, projectile, bilious or bloody emesis).
  2. Infants with failure to thrive, intrauterine growth retardation, haematochezia (blood in the stools), diarrhoea (watery stools that takes the shape of a container > 12x in breastfed and >5 in partially breastfed infants daily), or fever (38.0 degrees), as reported by parents/caregivers/legal guardians.
  3. Infants with congenital heart disease, immunodeficiency, asplenia, cancer, cystic fibrosis, and those with liver disease.
  4. Infants with reported exposure to probiotics in the 7 days prior to Screening Visit (V1) and throughout the study period.
  5. Infants with reported exposure to oral and/or systemic antibiotics in the 7 days prior to Screening Visit (V1) and throughout the study period.
  6. Infants with reported exposure to medications, therapies, or products with the aim to relieve infantile colic including proton pump inhibitors in the 7 days prior to Screening Visit (V1) and throughout the study period.
04

Study design

Phase
Not applicable
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Quadruple (Participant, Care provider, Investigator, Outcomes assessor)
Enrollment
102 participants (estimated)

Study arms

  • Experimental
    L. reuteri

    5 drops administered once daily for 21 days

    Dietary Supplement: L. reuteri

  • Placebo comparator
    Placebo

    5 drops administered once daily for 21 days

    Dietary Supplement: Placebo

Interventions

  • Dietary supplementL. reuteri

    Dietary supplementation with drops containing L. reuteri once daily for 21 consecutive days

  • Dietary supplementPlacebo

    Dietary supplementation with placebo drops identical to those containing L. reuteri once daily for 21 consecutive days

05

What researchers measure

Primary outcomes

  1. Crying and fussing time

    Change in daily crying and fussing time measured by Baby Day Diary

    Time frame: From baseline to Day 7

Secondary outcomes

  1. Crying and fussing time

    Change in mean daily crying and fussing time measured by Baby Day Diary

    Time frame: From baseline to Day 3, Day 5, Day 14, Day 21

  2. Crying time

    Change in mean daily crying time measured by Baby Day Diary

    Time frame: From baseline to Day 3, Day 5, Day 7, Day 14, Day 21

  3. Number of responders

    Proportion of responders defined as reduction of daily average crying time with 50 % compared to baseline

    Time frame: From baseline to Day 3, Day 5, Day 7, Day 14, Day 21

  4. Family quality of life (family QoL)

    Change in family QoL measured by the PedsQL Family Impact Module (Acute) - Total Score

    Time frame: From baseline to Day 7 and Day 21

  5. Sleeping time

    Change in mean sleeping time measured by Baby Day Diary

    Time frame: From baseline to Day 3, Day 5, Day 7, Day 14, Day 21

  6. Maternal depression

    Change in maternal depression measured by the Edinburgh Postnatal Depression Scale Total Score

    Time frame: From baseline to Day 7 and Day 21

06

Study locations

2 of 2 sites recruiting
  • Atlantia Clinical Trials
    Cork, Ireland
    • Aisling Harrington · Contact
    Recruiting
  • Clinical Trail Consultants AB
    Uppsala, Sweden
    • Kristina Ocklind · Contact
    Recruiting
07

Registry details

Key details

Study ID
NCT05512234
Lead sponsor
BioGaia AB
Collaborators
Atlantia Food Clinical Trials, CTC Clinical Trial Consultants AB
Responsible party
Sponsor
First posted
Aug 23, 2022
Start date
Jul 13, 2022
Primary completion
Oct 30, 2025 (estimated)
Completion
Oct 30, 2025 (estimated)
Last update
Sep 22, 2025

Study contacts

Caroline Linninge, PhD
Contact
cli@biogaia.se
+46 703811942
Jenny Palming, PhD
study director · BioGaia AB

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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