An observational study in Urea in Blood; High, sponsored by Assiut University. Completed at 1 site in Egypt. Open to participants aged 5 Months to 65 Years. Per ClinicalTrials.gov, last updated 2019-07-16.
Sponsored by Assiut University · Observational
Antibacterial properties of urea was tested against 35 isolates from ocular infections.
In vitro susceptibility testing was performed with commercially available antibiotic discs, homemade urea discs saturated with urea solution at a concentration of 96 mmol. The weight of urea in one drop = 0.37 mg, in one ml = 10.34 mg (5 microliter/ disc) discs and combined discs (antibiotic discs to which urea solution was added). Combined discs provided better in vitro susceptibility results than antibiotics alone.
Scanning electron micrographs of bacterial isolates incubated overnight with urea solution showed marked disruption of bacterial aggregative patterns and irregularities of their cell walls.
Western blotting showed marked reduction in total proteins in bacterial isolates incubated overnight with urea solution. These findings may explain the possible mechanisms for antibacterial activity of urea solution as a result of protein denaturation.
53 studies on the registry are indexed under Uremia; 10 are open to participants now.
This study's enrollment of 90 is below the median of 105 across 12 observational studies indexed under Uremia.
Browse Uremia studies →Assiut University is the lead sponsor of 5,078 studies on the registry; 2,178 are open to participants now.
Of its 14 completed or terminated interventional studies of FDA-regulated products, 0 (0%) have results posted.
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individuals with any ocular infection
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Exclusion Criteria:
Activity of Urea Solution against Ocular Bacterial Isolates
Biological: Antibacterial Activity
Antibacterial Activity of Urea Solution against Ocular Bacterial Isolates
Antibacterial effect of Urea Solution against Ocular Bacterial
Samples were both inoculated on Muller Agar (Sigma-Aldrich) plates and directly examined under the microscope using Gram stained smears. Inoculated plates were incubated at 37oC for 48 hrs. Gram negative isolates were sub-cultured on MacConkey's, Eosin Methelyene Blue (EMB) and Cystine Lactose Electrolyte Deficient (CLED) agar plates (Sigma-Aldrich). Gram positive isolates were sub-cultured on Mannitol Salt agar plates (Sigma-Aldrich).
Time frame: 6 month
Plan to share: Undecided
No publications or documents are linked to this record.
Ran from Nov 2017 to primary completion in Nov 2018, 12 months.
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This study is completed, as verified in Jul 2019. You cannot join it, but the record below documents what was studied.
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