Comprehensive Omics Analysis of Pediatric and Adult Solid Tumors and Establishment of a Repository for Related Biological Studies

Background:

-Laboratory-based investigations have contributed to an improved understanding of the biology of cancer and to the development of new therapies for pediatric malignancies.

Objectives:

-Systematic Molecular, Genomic, Proteomic, Metabolomic ("Omic") and other profiling for enrolled subjects where omics refers to mechanistic studies using a set of advanced technologies to capture comprehensive aspects of biological systems providing insights into structure, function and interactions to understand these complex processes and diseases better.

Eligibility:

Pediatric or Adult subjects of any age with one of the following:

• Diagnosis of any tumor or malignancy, pre-malignant disorder or suspected cancer susceptibility familial syndromes, regardless of age
• Individuals without malignancy undergoing surgery, other treatment such as apheresis or normal well visit or routine blood draw as part of standard of care.
• Biological relatives of a subject with a tumor or malignancy (pediatric or adult) or with suspected familial cancer syndrome. Patients enrolled in an approved companion protocol
• Blood and/or tissue specimens that have been previously collected and are available for research analysis
• Biospecimens can be collected with minimal additional risk to the subject during sampling or procedures required for routine patient care.

Design:

• This study will allow for the collection of specimens for a Tissue Repository, and for designated sample investigations including systematic molecular, genomic and proteomic (Omic) profiling, and growth factor and cellular profile investigations.

• Testing activities may include:

  • DNA, RNA and protein extracted from a section of tumor and normal samples; the remainder will be stored.
  • Germ line DNA and RNA extracted from lymphocytes or other normal uninvolved tissue
  • Germ line DNA will be extracted from lymphocytes or other normal uninvolved tissue of relatives of the subject.
  • Xenografts, explant and cell lines established from tumor, pre-malignant and normal samples
  • Tumor samples and samples for circulating tumor cells sent for the establishment of Xenografts and single cell suspension of tumor for drug testing or for monitoring cell free DNA over time
  • Omics (Genomics and Proteomic) studies will be performed
  • Growth factor and cellular profile investigations of bone marrow-derived cell populations to include quantification of hematopoietic progenitor cells (HPCs), endothelial progenitor cells (EPCs), and mesenchymal progenitor cells (MPCs), levels of matrix metalloprotease 2 and 9 (MMP2) and (MMP9), gene expression, growth factor and microvesicle analysis and bone marrow analysis of progenitor cells in blood and tissue.
  • Research tests described in active IRB approved protocols.
  • Immune profiling and stromal profiling of blood, tumor, and normal tissues
  • Immune function studies from blood and normal tumor tissues.
• Utilizing an oversight committee to oversee the receipt and the distribution of unlinked tissues to other investigators.
• Qualitative methodologies will be used to ascertain knowledge, attitudes, beliefs, and behaviors in 25-35 parents/caregivers at NIH concerning the anticipated use of NGS for diagnosing and directing therapy for pediatric cancer and how incidental findings might be returned.
• Expected accrual of 100-150 patients per year.