An interventional study of root canal treatment and samples from Gingival crevicular fluid in Irreversible Pulpitis (Toothache), sponsored by Ain Shams University. Completed at 1 site in Egypt. Open to participants aged 18 Years to 50 Years. Per ClinicalTrials.gov, last updated 2026-05-18.
Sponsored by Ain Shams University · Not applicable, Interventional, and Treatment
The aim of the present study was to Correlate between the clinical presentation of pulp and the biological mediators in gingival crevicular fluid and the inflamed pulpal tissue.
142 studies on the registry are indexed under Toothache; 28 are open to participants now.
This study's enrollment of 36 is below the median of 84 across 123 interventional studies indexed under Toothache.
Browse Toothache studies →Ain Shams University is the lead sponsor of 1,950 studies on the registry; 429 are open to participants now.
Of its 35 completed or terminated interventional studies of FDA-regulated products, 0 (0%) have results posted.
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Go through the criteria one by one — yes, no or not sure — and take the list to your doctor or the study team. Kept in this browser only.
Systemically healthy male and female individuals aged between 18 and 50 years
No physical or mental disabilities
No underlying systemic diseases
Mandibular permanent premolar teeth diagnosed with symptomatic irreversible pulpitis with apical periodontitis
Mandibular permanent premolar teeth diagnosed with symptomatic irreversible pulpitis without apical periodontitis
Diagnosis based on the terminology of the American Association of Endodontists (2009)
Patients who provided written
Fully developed teeth with no previous pulp exposure
Positive response to cold testing
Positive response to electric pulp testing
Exclusion Criteria
Requirement for antibiotic prophylaxis
Diabetes or hematologic disorders
Use of anti-inflammatory drugs, antibiotics, or antidepressants within the previous four weeks
Pregnancy at the time of treatment
Heavy plaque or calculus accumulation
Gingival redness and bleeding
Severe gingivitis or generalized periodontitis
Gingival pocket depths greater than 3 mm
Negative response to sensibility testing (cold test or electric pulp testing)
Presence of apical radiolucency (PAI > 2)
Previously initiated endodontic treatment
Internal or external root resorption
History of trauma
Severe calcific metamorphosis with obliterated root canal spaces on periapical radiographs
Vertical root fractures
Mobility greater than Grade I (Miller's mobility index)
Swelling or sinus tract associated with the treated tooth
Presence of periodontal-endodontic lesions
Teeth that could not be properly isolated with a dental dam
Collection of less than 10 µL of blood from each site
Conflicting clinical and radiographic findings preventing clear group assignment
No bleeding from one or more canal orifices (possible partial necrosis)
Underlined terms in the criteria open these too. Explained by clinicaltrials.gg, not part of the registry record. The study team decides who can take part.
Before you join, the study team explains what taking part involves — its purpose, what happens, the risks and possible benefits — and answers your questions, so you can decide freely. Consent is usually recorded on a form (the informed consent form, ICF) signed by you or, where the law allows, by someone legally authorised to decide for you.
You can keep asking questions during the study, you are told of new information that matters, and you can choose to leave at any time.
Informed consent
Before you join, the study team explains what taking part involves — its purpose, what happens, the risks and possible benefits — and answers your questions, so you can decide freely. Consent is usually recorded on a form (the informed consent form, ICF) signed by you or, where the law allows, by someone legally authorised to decide for you.
You can keep asking questions during the study, you are told of new information that matters, and you can choose to leave at any time.
Explained by clinicaltrials.gg, not part of the registry record. The study team decides who can take part.
root canal treatment and samples of biomarkers
Procedure: root canal treatment · Diagnostic Test: samples from Gingival crevicular fluid
samples of biomarkers
Procedure: root canal treatment · Diagnostic Test: samples from Gingival crevicular fluid
root canal treatment for 2 groups , irreversible pulpitis and the other group is irreversible pulpitis with apical periodontitis
samples from the gingival crevicular fluid before and after endodontic treatment and from contralateral tooth
biological markers level
TNF-alpha and sub P
Time frame: 2 months
Pain score through the Visual analogue scale
where the minimum reading is 0 and the maximum reading is 10
Time frame: 1 week
clinical presentation of irreversible pulpitis
Electric pulp tester
Time frame: 1 week
Plan to share: Undecided
No publications or documents are linked to this record.
This study is completed, as verified in May 2026. You cannot join it, but the record below documents what was studied.
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