CClinicalTrials.gg
Not yet recruitingNCT07466251PISTIAS-3Updated Jul 15, 2026

PCSK9 Inhibitor for Intracranial Atherosclerosis Related Acute Ischemic Stroke

A Phase 4 interventional study of Recaticimab plus standard therapy and Recaticimab's placebo in combination with standard therapy in Acute Ischemic Stroke AIS, Intracranial Atherosclerosis ICAS and Atherosclerotic Plaque, sponsored by Peking Union Medical College Hospital. Not yet recruiting at 19 sites in China. Open to participants aged 30 Years to 80 Years. Per ClinicalTrials.gov, last updated 2026-07-15.

Sponsored by Peking Union Medical College Hospital · Phase 4, Interventional, and Treatment

Phase
Phase 4
Study type
Interventional
Enrollment
1,212
Allocation
Randomized
Ages
30 Years to 80 Years
Sex
All
01

Study summary

This study is a prospective, multicenter, double-blind, randomized, placebo-controlled clinical trial designed to evaluate whether early administration of PCSK9 inhibitors can effectively improve functional outcomes at 90 days in patients with ischemic stroke (AIS) associated with intracranial atherosclerotic stenosis (ICAS), primarily assessed using the modified Rankin Scale at 90 days.

Read the detailed description

Acute ischemic stroke (AIS) remains one of the leading causes of death and disability worldwide. Although intravenous thrombolysis and endovascular therapies have significantly improved reperfusion success rates, three critical challenges persist in clinical practice that determine prognosis: First, only a limited proportion of patients can actually receive reperfusion therapy within the therapeutic time window; Second, even with successful reperfusion, secondary injuries such as microcirculatory perfusion failure, inflammatory cascades, and thrombus reformation may still occur. Third, acute phase fluctuations, particularly early neurological deterioration and the risk of early recurrence, remain prominent, directly limiting improvements in functional outcomes. 3 Intracranial atherosclerotic stenosis (ICAS) accounts for up to 50% of ischemic stroke etiologies in China. Its pathological chain-"plaque instability-thrombosis-microcirculatory impairment"-permeates both the acute and subacute phases, closely correlating with early recurrence and poor outcomes.Proprotein convertase subtilisin/kexin type 9 (PCSK9) classically promotes the degradation of low-density lipoprotein cholesterol (LDL-C) receptors and elevates LDL-C levels, thereby driving atherosclerosis progression. More importantly, growing basic and translational research suggests that PCSK9 may not only function as a "lipid metabolism protein" but also participate in processes such as endothelial activation, inflammatory cascades, platelet reactivity, and microcirculatory dysfunction. This positions it as a potential hub connecting the "plaque-thrombus-inflammation" axis. Consequently, PCSK9 inhibitors may offer additional neurovascular protective benefits beyond lipid-lowering effects during the acute phase of acute ischemic stroke (AIS).Existing basic and clinical evidence suggests that PCSK9 inhibitors may exert a combined intervention effect on key pathological processes driving atherosclerosis during the acute phase of AIS through a dual mechanism involving both lipid-dependent and non-lipid-dependent pathways. Mechanistic studies reveal that PCSK9 inhibition simultaneously modulates inflammatory responses, endothelial activation, dysfunction, and thrombogenic tendencies. In ischemia-reperfusion models, it demonstrates neuroprotective signaling by mitigating brain injury and improving neurological function. Clinically, large randomized trials confirm its ability to rapidly enhance lipid-lowering effects beyond statins and reduce ischemic stroke risk. Further translational evidence in cerebrovascular disease indicates that in symptomatic ICAS populations, PCSK9 inhibitor plus statin-enhanced lipid-lowering therapy reduces plaque burden, alleviates stenosis, and increases plaque stability. In the acute phase of AIS, early addition of PCSK9 inhibitors correlates with reduced neurological deterioration within 7 days, decreased recurrence risk within 30 days, and improved functional outcomes at 90 days. Collectively, this evidence chain spanning mechanisms to clinical practice provides clear scientific rationale and clinical necessity for adding PCSK9 inhibitors to the treatment of ICAS-associated AIS.This study is a nationwide, prospective, multicenter, double-blind, randomized, placebo-controlled clinical trial. It will enroll 1,212 patients meeting inclusion and exclusion criteria. Patients will be randomly assigned to two groups using a randomized allocation method: (1) Experimental group: 450 mg of Recaticimab for Injection (3 vials) administered as a single subcutaneous injection. (2) Control group: Placebo of Recaticimab for Injection 450 mg (3 vials) administered as a single subcutaneous injection. Each group will include 606 patients. Both groups will receive standard guideline-recommended treatment in addition to the study drug. Long-term efficacy was assessed through patient visits and evaluations at 0 hours, 24 hours (±2 or ±12 hours), 7 days (±2 days) or at discharge, 90 days (±7 days), and 1 year.Primary outcome measures included: Analysis based on the intention-to-treat principle using an ANCOVA model for all randomized patients with baseline HRMRI and a modified Rankin Scale (mRS) score of 0-2 at 90 days. Secondary outcome measures included: (1) mRS score of 0-1 at 90 days (indicating good patient function); (2) Distribution of 90-day mRS scores; (3) Any stroke (including ischemic and hemorrhagic) within 90 days; (4) Ischemic stroke within 90 days; (5) Composite vascular events (including stroke, myocardial infarction, and vascular death) within 90 days; (6) 90-day European Quality of Life 5-Dimension 5-Level Scale (EQ-5D-5L) score; (7) 90-day Barthel Index (BI) score.Details are provided in the "Outcome Measures" section.The sample size is calculated based on the primary outcome and a total of 1212 participants are anticipated. An independent Data Safety Monitoring Board will oversee the overall conduct of the trial.

02

Conditions studied

  • Acute Ischemic Stroke AIS
  • Intracranial Atherosclerosis ICAS
  • Atherosclerotic Plaque

Keywords

  • Intracranial Atherosclerosis
  • intracranial artery stenosis
  • atherosclerotic plaque
  • PCSK9 inhibitor
  • Recaticimab
03

Who can participate

Ages eligible
30 Years to 80 Years
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  1. Age 30 to 80 years, inclusive.
  2. Acute ischemic stroke with symptom onset within 72 hours before screening, diagnosed on the basis of neurological deficits and CT or MRI findings.
  3. National Institutes of Health Stroke Scale (NIHSS) score of 4 to 25 at hospital admission.
  4. CTA, DSA, or MRA evidence that the culprit intracranial artery has atherosclerotic stenosis of 50% to 99%.

Exclusion criteria

Exclusion Criteria

  1. Intracranial arterial stenosis attributable to a nonatherosclerotic cause, such as arterial dissection, moyamoya disease, or systemic vasculitis.
  2. Any definite source of cardioembolism, such as atrial fibrillation, a mechanical prosthetic heart valve, left ventricular thrombus, or patent foramen ovale.
  3. Clinical and imaging findings suggesting that the index ischemic event is primarily attributable to cerebral small vessel disease.
  4. Pre-stroke disability, defined as a modified Rankin Scale score of 2 or higher before the index ischemic event.
  5. Extensive cerebral infarction on CT or MRI, such as an Alberta Stroke Program Early CT Score (ASPECTS) below 6 or an infarct volume of 70 mL or greater.
  6. Previous stent implantation in the culprit vessel or planned stent implantation in the culprit vessel within 3 months after enrollment.
  7. Any intracranial hemorrhage within 3 months before enrollment.
  8. An intracranial tumor; or a cerebral aneurysm or arteriovenous malformation judged to require interventional treatment.
  9. Severe active bleeding tendency or coagulation disorder.
  10. Severe cardiac, hepatic, renal, or other major organ dysfunction.
  11. Treatment with a PCSK9 monoclonal antibody inhibitor within 1 month before enrollment or with a PCSK9-targeting small interfering RNA therapy within 6 months before enrollment.
  12. A definite contraindication to statin therapy or a history of statin intolerance.
  13. Pregnant or breastfeeding, or planning to become pregnant during the study.
  14. Current participation in another clinical trial.
04

Study design

Phase
Phase 4
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Quadruple (Participant, Care provider, Investigator, Outcomes assessor)
Enrollment
1,212 participants (estimated)

Study arms

  • Experimental
    Recaticimab plus standard therapy group

    Recaticimab in combination with standard therapy

    Drug: Recaticimab plus standard therapy

  • Placebo comparator
    Recaticimab's placebo in combination with standard therapy group

    Recaticimab's placebo in combination with standard therapy

    Drug: Recaticimab's placebo in combination with standard therapy

Interventions

  • DrugRecaticimab plus standard therapy

    Recaticimab (450 mg single dose, subcutaneous injection) combined with standard therapy recommended by the AHA/ASA Guidelines for Early Management of Acute Ischemic Stroke 2026 and the Chinese Guidelines for Diagnosis and Treatment of Acute Ischemic Stroke 2023.

  • DrugRecaticimab's placebo in combination with standard therapy

    The placebo and investigational ricaximab were identical in appearance, packaging, labeling, administration method, and dosing frequency, managed through a unified production and coding system. Standard treatment followed the recommendations outlined in the "AHA/ASA Guidelines for the Early Management of Acute Ischemic Stroke 2026" and the "Chinese Guidelines for the Diagnosis and Treatment of Acute Ischemic Stroke 2023."

05

What researchers measure

Primary outcomes

  1. Proportion of participants with functional independence, defined as a modified Rankin Scale score of 0-2, at Day 90

    The modified Rankin Scale (mRS) is a 7-category ordinal scale ranging from 0 (no symptoms) to 6 (death), with lower scores indicating better functional status. This outcome is the proportion of participants with an mRS score of 0-2, representing functional independence. Participants who die before the Day 90 assessment will be assigned an mRS score of 6. The assessment will be performed by trained assessors blinded to treatment allocation.

    Time frame: Day 90 (±7 days) after randomization

Secondary outcomes

  1. Proportion of participants with an excellent functional outcome, defined as an mRS score of 0-1, at Day 90

    The mRS ranges from 0 (no symptoms) to 6 (death), with lower scores indicating better functional status. This outcome is the proportion of participants with an mRS score of 0-1, representing an excellent functional outcome. Participants who die before the assessment will be assigned an mRS score of 6.

    Time frame: Day 90 (±7 days) after randomization

  2. Distribution of modified Rankin Scale (mRS) scores at Day 90

    The ordinal distribution of mRS scores across all seven categories will be assessed. The mRS ranges from 0 (no symptoms) to 6 (death), with lower scores indicating better functional status. The outcome will evaluate a shift toward better functional outcomes across the full mRS distribution.

    Time frame: Day 90 (±7 days) after randomization

  3. Incidence of any stroke through Day 90

    Proportion of participants who experience an adjudicated stroke after randomization, including either ischemic stroke or hemorrhagic stroke. Suspected events will be reviewed by an independent Clinical Event Committee blinded to treatment allocation.

    Time frame: From randomization through Day 90

  4. Incidence of ischemic stroke through Day 90

    Proportion of participants who experience an adjudicated ischemic stroke after randomization. Ischemic stroke is defined as a new focal neurological injury attributable to cerebral ischemia, supported by clinical findings and/or imaging evidence of acute cerebral infarction, and not explained by a nonischemic cause. Events will be adjudicated by an independent blinded Clinical Event Committee.

    Time frame: From randomization through Day 90

  5. Incidence of composite vascular events through Day 90

    Proportion of participants who experience at least one component of the composite endpoint after randomization. The composite endpoint includes any stroke, myocardial infarction, or vascular death. Events will be adjudicated by an independent Clinical Event Committee blinded to treatment allocation.

    Time frame: From randomization through Day 90

  6. Barthel Index score at Day 90

    Activities of daily living will be assessed using the Barthel Index. The total score ranges from 0 to 100, with higher scores indicating greater independence in activities of daily living and better functional status.

    Time frame: Day 90 (±7 days) after randomization

  7. Health-related quality of life assessed using the EQ-5D-5L at Day 90

    Health-related quality of life will be assessed using the European Quality of Life 5-Dimension 5-Level instrument. The descriptive system assesses mobility, self-care, usual activities, pain/discomfort, and anxiety/depression, with five levels of severity for each dimension. Overall self-rated health will also be assessed using the EQ visual analogue scale, ranging from 0 (worst imaginable health) to 100 (best imaginable health).

    Time frame: Day 90 (±7 days) after randomization

  8. National Institutes of Health Stroke Scale score at Day 7 or hospital discharge

    Neurological impairment will be assessed using the National Institutes of Health Stroke Scale (NIHSS). The NIHSS ranges from 0 to 42, with higher scores indicating greater neurological impairment.

    Time frame: Day 7 (±2 days) after randomization or at hospital discharge, whichever occurs first

  9. Change from baseline in NIHSS score at Day 7 or hospital discharge

    Change in NIHSS score will be calculated as the follow-up NIHSS score minus the baseline NIHSS score. The NIHSS ranges from 0 to 42, with higher scores indicating greater neurological impairment. A negative change indicates neurological improvement, whereas a positive change indicates neurological worsening.

    Time frame: From baseline to Day 7 (±2 days) after randomization or hospital discharge, whichever occurs first

  10. Proportion of participants achieving an LDL-C level below 1.4 mmol/L at Day 7 or hospital discharge

    Proportion of participants whose measured low-density lipoprotein cholesterol (LDL-C) concentration is below 1.4 mmol/L at the scheduled assessment.

    Time frame: Day 7 (±2 days) after randomization or at hospital discharge, whichever occurs first

  11. Proportion of participants achieving an LDL-C level below 1.8 mmol/L at Day 7 or hospital discharge

    Proportion of participants whose measured low-density lipoprotein cholesterol (LDL-C) concentration is below 1.8 mmol/L at the scheduled assessment.

    Time frame: Day 7 (±2 days) after randomization or at hospital discharge, whichever occurs first

  12. Change from baseline in LDL-C level at Day 7 or hospital discharge

    Change in LDL-C concentration will be calculated as the follow-up LDL-C value minus the baseline value and reported in mmol/L. A negative value indicates a reduction in LDL-C from baseline.

    Time frame: From baseline to Day 7 (±2 days) after randomization or hospital discharge, whichever occurs first

Other outcomes

  1. Change from baseline in NIHSS score at 24 hours

    Change in NIHSS score will be calculated as the 24-hour NIHSS score minus the baseline NIHSS score. The NIHSS ranges from 0 to 42, with higher scores indicating greater neurological impairment. A negative change indicates neurological improvement.

    Time frame: From baseline to 24 hours after randomization, with an allowable assessment window of -2 to +12 hours

  2. Cerebral infarct volume at Day 7 or hospital discharge

    Cerebral infarct volume will be quantified in milliliters using follow-up CT or MRI and assessed by a blinded core imaging laboratory. Lower infarct volumes indicate less extensive ischemic brain injury.

    Time frame: Day 7 (±2 days) after randomization or at hospital discharge, whichever occurs first

  3. Cerebral perfusion parameters at Day 7 or hospital discharge

    Prespecified quantitative cerebral perfusion parameters derived from CT perfusion or MR perfusion imaging will be assessed by a blinded core imaging laboratory.

    Time frame: Day 7 (±2 days) after randomization or at hospital discharge, whichever occurs first

  4. Serum interleukin-6 concentration at Day 7 or hospital discharge

    Serum interleukin-6 concentration will be measured as a marker of systemic inflammation. Higher concentrations indicate greater inflammatory activity. Testing will be performed according to the prespecified laboratory procedures.

    Time frame: Day 7 (±2 days) after randomization or at hospital discharge, whichever occurs first

  5. High-sensitivity C-reactive protein concentration at Day 7 or hospital discharge

    High-sensitivity C-reactive protein concentration will be measured as a marker of systemic inflammation. Higher concentrations indicate greater inflammatory activity.

    Time frame: Day 7 (±2 days) after randomization or at hospital discharge, whichever occurs first

  6. Incidence of any stroke through 1 year

    Proportion of participants who experience an adjudicated ischemic or hemorrhagic stroke after randomization. Events will be reviewed by an independent Clinical Event Committee blinded to treatment allocation.

    Time frame: From randomization through 1 year

  7. Incidence of ischemic stroke through 1 year

    Proportion of participants who experience an adjudicated ischemic stroke after randomization. Events will be reviewed by an independent Clinical Event Committee blinded to treatment allocation.

    Time frame: From randomization through 1 year

  8. Incidence of composite vascular events through 1 year

    Proportion of participants who experience at least one component of the composite endpoint, defined as any stroke, myocardial infarction, or vascular death. Events will be adjudicated by an independent blinded Clinical Event Committee.

    Time frame: From randomization through 1 year

  9. Health-related quality of life assessed using the EQ-5D-5L at 1 year

    Health-related quality of life will be assessed using the EQ-5D-5L descriptive system, covering mobility, self-care, usual activities, pain/discomfort, and anxiety/depression. Overall self-rated health will also be assessed using the EQ visual analogue scale ranging from 0 to 100, with higher scores indicating better perceived health.

    Time frame: At 1 year after randomization

  10. Barthel Index score at 1 year

    Activities of daily living will be assessed using the Barthel Index. The total score ranges from 0 to 100, with higher scores indicating greater independence and better functional status.

    Time frame: At 1 year after randomization

  11. Incidence of moderate-to-severe symptomatic intracranial hemorrhage within 72 hours

    Proportion of participants with symptomatic intracranial hemorrhage according to the modified Heidelberg criteria. The event must include imaging-confirmed intracranial hemorrhage, an increase of at least 4 points in the total NIHSS score compared with baseline or a previously recorded NIHSS score, and neurological deterioration that cannot be explained by a cause other than the intracranial hemorrhage. Qualifying hemorrhage types include parenchymal hematoma type 1 or 2, remote intracranial hemorrhage, subarachnoid hemorrhage, intraventricular hemorrhage, or subdural hemorrhage.

    Time frame: From randomization through 72 hours after randomization

  12. All-cause mortality through Day 90

    Proportion of participants who die from any cause after randomization and through Day 90. Death will be recorded as an mRS score of 6.

    Time frame: From randomization through Day 90

  13. Incidence of adverse events through Day 90

    Proportion of participants who experience at least one adverse event after administration of the study intervention. Adverse events will be recorded with respect to onset, duration, severity, relationship to the study intervention, action taken, and outcome, and will be coded using the Medical Dictionary for Regulatory Activities.

    Time frame: From administration of the study intervention through Day 90 after randomization

  14. Incidence of serious adverse events through Day 90

    Proportion of participants who experience at least one serious adverse event. A serious adverse event is an event that results in death, is life-threatening, requires inpatient hospitalization or prolongation of hospitalization, results in persistent or significant disability, causes a congenital anomaly or birth defect, or is considered an important medical event requiring intervention to prevent a serious outcome.

    Time frame: From administration of the study intervention through Day 90 after randomization

06

Study locations

19 sites
  • Chinese PLA General Hospital
    Beijing, Beijing Municipality 100853, China
    • Shiwen Wu, MD · Contact · wu_shiwen@outlook.com · 86+13910238117
    • Shiwen Wu, MD · Principal investigator
  • The Third Affiliated Hospital of Sun Yat-sen University, Yuedong Hospital
    Meizhou, Guangdong, China
  • Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine
    Cangzhou, Hebei, China
  • Peking University Third Hospital Qinhuangdao Hospital
    Qinhuangdao, Hebei, China
  • Hebei Provincial People's Hospital
    Shijiazhuang, Hebei 050051, China
  • Tangshan Worker's Hospital
    Tangshan, Hebei 063000, China
    • Baoquan Lu · Contact · balcom@163.com · 13930565557
    • Baoquan Lu, MD · Principal investigator
  • First Affiliated Hospital of Harbin Medical University
    Harbin, Heilongjiang, China
  • The first affiliated hospital of zhengzhou university
    Zhengzhou, Henan, China
  • Affiliated Taihe Hospital of Hubei University of Medicine
    Shiyan, Hubei, China
  • Zhongnan Hospital of Wuhan University
    Wuhan, Hubei, China
  • Baotou Central Hospital
    Baotou, Inner Mongolia 014000, China
  • Nanjing First Hospital
    Nanjing, Jiangsu 210000, China
  • Jining First People's Hospital
    Jining, Shandong 272000, China
  • Liaocheng People's Hospital
    Liaocheng, Shandong 252000, China
  • The Affiliated Hospital of Qingdao University
    Qingdao, Shandong, China
  • Weifang People's Hospital
    Weifang, Shandong 261000, China
  • Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, Beijing 100730
    Beijing, China
    • Weihai Xu, MD · Contact · xuwh@pumch.cn · 86+13651147766
    • Weihai Xu, MD · Principal investigator
  • Chongqing General Hospital
    Chongqing, China
  • Huashan Hospital, Fudan University
    Shanghai, China
07

References and documents

Publications

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Individual participant data

Plan to share: Undecided

08

Registry details

Key details

Study ID
NCT07466251
Lead sponsor
Peking Union Medical College Hospital
Collaborators
Chinese PLA General Hospital, Hebei General Hospital, Taihe Hospital, Weifang People's Hospital, Nanjing First Hospital, Nanjing Medical University, Baotou Central Hospital, Jining First People's Hospital, Liaocheng People's Hospital, Tangshan Worker's Hospital, Chongqing General Hospital, First Affiliated Hospital of Harbin Medical University, Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, The First Affiliated Hospital of Zhengzhou University, The Affiliated Hospital of Qingdao University, Zhongnan Hospital, Huashan Hospital
Responsible party
Wei-Hai Xu (MD & PhD, Peking Union Medical College Hospital) — Principal investigator
First posted
Mar 12, 2026
Start date
Sep 1, 2026 (estimated)
Primary completion
Dec 31, 2028 (estimated)
Completion
Dec 31, 2029 (estimated)
Last update
Jul 15, 2026

Study contacts

Weihai Xu, MD
Contact
xuwh@pumch.cn
86+13938912070
Yiyang Liu, PhD
Contact
liuyydoct@163.com
86+13938912070

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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This study is not yet recruiting, as verified in Apr 2026. You cannot join it, but the record below documents what was studied.

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