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CompletedNCT02460250FIBROSCAMEUpdated Oct 24, 2019

Evaluation of Liver Grafts With FibroScan® Before Organ Retrieval in Patients With Brain Death

An interventional study of Fibroscan in Tissue and Organ Harvesting, Liver Transplantation and Brain Death, sponsored by University Hospital, Limoges. Completed at 19 sites in France. Open to participants aged 18 Years and older. Per ClinicalTrials.gov, last updated 2019-10-24.

Sponsored by University Hospital, Limoges · Not applicable, Interventional, and Diagnostic

Phase
Not applicable
Study type
Interventional
Enrollment
740
Allocation
Not applicable
Ages
18 Years and older
Sex
All
01

Study summary

According to the French Biomedicine Agency annual report on retrieval activities and transplants, 1,164 liver transplants were performed in 2011 and 1,161 in 2012. If the amount of brain death donors and retrieved liver grafts appears relatively stable, it remains clearly insufficient compared to the increasing number of patients on the waiting list for liver transplantation (2,462 in 2011). The median time on the waiting list before liver transplantation which was established from the cohort of patients registered between 2007 and 2011 (excluding patients registered for emergency transplantation and for living related-donor transplantation) increased significantly from 4.4 months between 2007 and 2009 to 6.6 months between 2010 and 2011. In order to compensate for the lack of liver grafts, donors acceptance criteria were broadened. For example, alternative transplantation lists were created with liver grafts coming from so-called "marginal" donors. However, despite these efforts, livers were retrieved on only two out of three brain death donors, i.e. in 1,572 and 1,589 organ donors in 2011 and 2012, respectively. This is unfortunately not enough to meet the increasing needs in liver grafts and a growing number of patients wait each year for transplant. Strategic lines of improvement were defined in order to meet the "2012-2016 transplant perspective" which targets 5,700 transplants carried out in 2015 (+5% every year, all transplants included, with 5,023 transplants in 2012).

According to the last consensus conference on liver transplantation of the HAS (French High Authority of Health) the assessment of the degree of macrovacuolar and microvacuolar steatosis determines the possibility to retrieve the graft or not. Liver steatosis consists in an accumulation of fatty droplets in hepatocytes. Its prevalence is high, ranging from 16% to 31% in the general population, and increases up to 46% in heavy drinkers and to 50-80% in the obese population. Steatosis results mostly from alcohol consumption and from metabolic syndrome (obesity, type 2 diabetes, hypertriglyceridemia) called non-alcoholic fatty liver disease (NAFLD), and is more rarely secondary to viral hepatitis or exposure to certain medications. NAFLD involves up to 30% of the population in Western countries and its prevalence is increasing. NAFLD may lead to asymptomatic steatosis, but also to steatohepatitis or advanced fibrosis including cirrhosis and its complications Accordingly, the improvement of liver grafts selection based on objective quantitative criteria which takes into account the degree of liver steatosis appears crucial to increase the number of hepatic transplants.

Read the detailed description

Medical procedure: Fibroscan® based on vibration control transient elastography (VCTETM) with evaluation of controlled attenuation parameter (CAP™) by ultrasounds (Echosens, Paris, France).

02

Conditions studied

  • Tissue and Organ Harvesting
  • Liver Transplantation
  • Brain Death

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Keywords

  • Tissue and Organ Harvesting
  • Liver graft
  • Brain Death
  • FibroScan®
03

Who can participate

Ages eligible
18 Years and older
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  • Patients older than 18 years with recognized clinical brain death confirmed by clinical examination, who are considered for organ retrieval and clinically stable to achieve the retrieval procedure

Exclusion criteria

Exclusion Criteria:

  • Personal objection to organ retrieval and clinical research expressed during lifetime and registered in the Registre National des Refus (French registration of all refusals to organ donation)
  • Family objection to liver retrieval after donor's death
  • Ongoing pregnancy when brain death is declared
  • For FT and ST measurements included in the panel FibroMaxTM, 4 exclusion criteria are defined specifically (Acute hepatitis or cytolysis with ALT higher than 622 IU/L, acute or chronic hemolysis, extrahepatic cholestasis, sepsis)
04

Study design

Phase
Not applicable
Primary purpose
Diagnostic
Allocation
Not applicable
Intervention model
Single group
Masking
None (open label)
Enrollment
740 participants (actual)

Study arms

  • Experimental
    Fibroscan

    All included patients will undergo a Fibroscan (either Fibroscan Touch model or 402 model which enable CAPTM data extraction) once all eligibility criteria have been checked. Liver recipients will be followed up during one year. Biological and medical data used by all transplant sites for the follow-up of transplant patient will be collected

    Procedure: Fibroscan

Interventions

  • ProcedureFibroscan

    All included patients will undergo a Fibroscan (either Fibroscan Touch model or 402 model which enable CAPTM data extraction) once all eligibility criteria have been checked.

05

What researchers measure

Primary outcomes

  1. Controlled Attenuation Parameter™ (CAP™) measurement

    Evaluation of the diagnostic accuracy of the CAP™ measured with Fibroscan® to objectively reflect the degree of liver steatosis, a parameter which can be used as an aid in selecting liver grafts before retrieval in donors with brain death (DBD).

    Time frame: 1 Day

Secondary outcomes

  1. Liver Stiffness (LS) measurement

    Evaluation of the diagnostic accuracy of LS measured with Fibroscan® to objectively reflect the degree of fibrosis and steatosis, a parameter which can be used as an aid in selecting liver grafts before retrieval in DBD

    Time frame: 1 Day

  2. Controlled Attenuation Parameter™ (CAP™) measurement

    Determination of the prognostic value of CAP™ in terms of survival of liver grafts at one week after transplantation

    Time frame: 1 week

  3. Liver Stiffness (LS) measurement

    Determination of the prognostic value of LS in terms of survival of liver grafts at one week after transplantation

    Time frame: 1 week

  4. Liver Stiffness (LS) measurement

    Determination of the prognostic value of LS in terms of survival of liver grafts at one month after transplantation

    Time frame: 1 month

  5. Controlled Attenuation Parameter™ (CAP™) measurement

    Determination of the prognostic value of CAP™ in terms of survival of liver grafts at one month after transplantation

    Time frame: 1 month

  6. Liver Stiffness (LS) measurement

    Determination of the prognostic value of LS in terms of survival of liver grafts at one week, one month and one year after transplantation

    Time frame: 1 year

  7. Controlled Attenuation Parameter™ (CAP™) measurement

    Determination of the prognostic value of CAP™ in terms of survival of liver grafts at one week, one month and one year after transplantation

    Time frame: 1 year

  8. Fibrosis measurement

    evaluate the diagnostic a accuracy of FibroTest in selecting liver grafts before retrieval in DBD by comparing with 1) histological data obtained with liver biopsy (degree of steatosis, degree of fibrosis) and with 2) CAPTM/LS

    Time frame: 1 Day

  9. Steatosis measurement

    evaluate the diagnostic a accuracy of SteatoTest in selecting liver grafts before retrieval in DBD by comparing with 1) histological data obtained with liver biopsy (degree of steatosis, degree of fibrosis) and with 2) CAPTM/LS

    Time frame: 1 Day

  10. Fibrosis measurement

    Determination of the short-term prognostic values of FibroTest in terms of survival of liver grafts at one month after transplantation

    Time frame: 1 month

  11. Steatosis measurement

    Determination of the short-term prognostic values of SteatoTest in terms of survival of liver grafts at one month after transplantation

    Time frame: 1 month

  12. Fibrosis measurement

    Determination of the short-term prognostic values of FibroTest in terms of survival of liver grafts at one year after transplantation

    Time frame: 1 year

  13. Steatosis measurement

    Determination of the short-term prognostic values of SteatoTest in terms of survival of liver grafts at one year after transplantation

    Time frame: 1 year

06

Study locations

19 sites
  • University Hospital
    Amiens, 80054, France
  • University Hospital
    Angers, 49100, France
  • University Hospital
    Besançon, 25030, France
  • University Hospital
    Bordeaux, 33000, France
  • University Hospital
    Brest, 29200, France
  • University Hospital
    Caen, 14033, France
  • Estaing University Hospital
    Clermont Ferrand, 63100, France
  • AP-HP Henri MONDOR
    Creteil, 94010, France
  • University Hospital
    Grenoble, 38043, France
  • University Hospital
    Lille, 59037, France
  • University Hospital
    Limoges, 87042, France
  • Croix Rousse Hospital
    Lyon, 69004, France
  • Nice University Hospital
    Nice, 06000, France
  • AP-HP Kremlin Bicêtre
    Paris, 84270, France
  • University Hospital
    Poitiers, 86021, France
  • Univesity Hospital
    Reims, 51100, France
  • University Hospital
    Rennes, 35033, France
  • University Hospital
    Tours, 37044, France
  • University Hospital
    Vandoeuvre-les-nancy, 54500, France
07

Registry details

Key details

Study ID
NCT02460250
Lead sponsor
University Hospital, Limoges
Responsible party
Sponsor
First posted
Jun 2, 2015
Start date
Feb 2015
Primary completion
Nov 9, 2017
Completion
Nov 9, 2018
Last update
Oct 24, 2019

Study contacts

Nicolas PICHON, MD
principal investigator · University Hospital, Limoges

Oversight

Data monitoring committee
No
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