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Not yet recruitingNCT07805746CytokinegreffeUpdated Sep 4, 2026

Peri-graft Cytokine Profile: Influence of Hematologic Disease and Donor Type

An observational study in Myelofibrosis, Acute Leukemia and Myelodysplastic Syndrome, sponsored by Assistance Publique - Hôpitaux de Paris. Not yet recruiting. Open to participants aged 18 Years and older. Per ClinicalTrials.gov, last updated 2026-09-04.

Sponsored by Assistance Publique - Hôpitaux de Paris · Observational

Study type
Observational
Model
Cohort
Time perspective
Prospective
Enrollment
96
Ages
18 Years and older
Sex
All
01

Study summary

Cytokine profiles in the early post-transplant phase may influence the Development of early post-transplant inflammatory or immunological complications. This study propose to analyze these cytokine profiles based on the disease (myelofibrosis or other hematologic malignancies) and on the donor type (haploidentical, genotypically identical, or phenotypically identical). Patients with myelofibrosis have an inflammatory cytokine profile prior to transplantation. Patients who undergo haploidentical allogeneic hematopoietic stem cell transplantation (HSCT) most often experience a post-transplant cytokine release syndrome, and the question is whether these two conditions-myelofibrosis and haploidentical transplantation-increase the probability of an early cytokine release, potentially putting these patients at greater risk for acute GVHD than patients who undergo transplantation under other conditions or who have other diseases. In the prospective Phase 2 "FIBRAPLO" protocol (ClinicalTrials.gov ID NCT04728490), 28 patients with myelofibrosis received an allogeneic hematopoietic stem cell transplantation from a haploidentical donor, and serum samples were collected at Day-7 before transplantation, Day 0 and Day+7 after transplantation to analyse cytokine profiles around the time of allogeneic HSCT.

The purpose of the present study is to match these FIBRAPLO patients (patients with myelofibrosis who received an allograft from a haploidentical donor) with patients without myelofibrosis who received a haploidentical transplant, in order to determine whether these profiles are specific to this population. The aim is to compare these profiles to other scenarios: patients with or without myelofibrosis who received transplants from 10/10 or geno-identical donors.

This study propose to prospectively collect blood samples at time points (D-7, D0, and D7) from control patients :

  • receiving a haplo-identical allogeneic transplant for another condition (acute leukemia, myelodysplastic syndrome),
  • a geno-identical or 10/10 matched unrelated allogeneic transplant, for myelofibrosis or for other condition (acute leukemia, myelodysplastic syndrome)
02

Conditions studied

  • Myelofibrosis
  • Acute Leukemia
  • Myelodysplastic Syndrome
  • Hematopoietic Stem Cell Transplant (HSCT)

Keywords

  • Peri-graft cytokine Profile
  • Influence of Hematologic Disease
  • Influence of Donor Type
03

Who can participate

Ages eligible
18 Years and older
Sexes eligible
All
Accepts healthy volunteers
No
Sampling method
Non-probability sample

Study population

Adult patient hospitalized for an allogeneic hematopoietic stem cell (HSC) transplantation for myelofibrosis, acute leukemia (AL), or myelodysplastic syndrome (MDS)

Inclusion criteria

  • Patients aged 18 years and older
  • With one of the following conditions:

    • Acute leukemia (AL)
    • Myelofibrosis (MF)
    • Myelodysplastic syndrome (MDS)
  • Indicated for allogeneic hematopoietic stem cell (HSC) transplantation from:

    • a haploidentical donor with post-transplant cyclophosphamide OR
    • a geno-identical donor, without post-transplant cyclophosphamide OR
    • a pheno-identical donor, without post-transplant cyclophosphamide

Exclusion criteria

Exclusion Criteria:

  • Lymphoma
  • Non-malignant disease
  • Patient's refusal to participate in this study
  • Patient's refusal to have their data recorded in the EBMT registry
  • Transplant from an unrelated donor 9/10
  • Person under legal guardianship or curatorship, or unable to give informed consent
  • Person subject to judicial protective measures, or deprived of liberty by a judicial or administrative decision
04

Study design

Observational model
Cohort
Time perspective
Prospective
Enrollment
96 participants (estimated)
Patient registry
No

Groups and cohorts

  • MF-Haplo

    Patients with myelofibrosis receiving a haplo-identical allogeneic transplant

    Other: Blood sampling

  • MF-10/10

    Patients with myelofibrosis receiving a geno- or pheno-identical allogeneic transplant

    Other: Blood sampling

  • AL-haplo

    Patients with acute leukemia or myelodysplastic syndrome receiving a haplo-identical allogeneic transplant

    Other: Blood sampling

  • AL-10/10

    Patients with acute leukemia or myelodysplastic syndrome a geno- or pheno-identical allogeneic transplant

    Other: Blood sampling

Interventions

  • OtherBlood sampling

    Blood sampling at D-7, D0 and D+7 from allogeneic transplantation

05

What researchers measure

Primary outcomes

  1. GVHD panel 2 cytokines in haplo-identical transplantation

    Comparison of ST2 and REG3α dosages between MF-haplo and AL-haplo patients

    Time frame: At day 7

Secondary outcomes

  1. Cytokine level

    Changes in cytokine levels before and after transplantation comparison according the hematologic condition and the type of allogeneic transplantation between Day -7 and Day 0, and between Day 0 and Day +7

    Time frame: Up to day 7

  2. Cytokine profile

    Peri-graft cytokine profile, comparison according the hematologic condition and the type of allogeneic transplantation

    Time frame: 7 days before allogeneic transplantation

  3. Cytokine profile

    Peri-graft cytokine profile, comparison according the hematologic condition and the type of allogeneic transplantation

    Time frame: the day of allogeneic transplantation

  4. Cytokine profile

    Peri-graft cytokine profile, comparison according the hematologic condition and the type of allogeneic transplantation

    Time frame: 7 days after allogeneic transplantation

  5. Occurrence of early acute GVHD

    Occurrence of early acute GVHD ; comparison according the hematologic condition and the type of allogeneic transplantation

    Time frame: 45 days after transplantation

  6. Overall survival

    Overall survival; defined as the time from Day 0 to death from any cause ; comparison according the hematologic condition and the type of allogeneic transplantation

    Time frame: At 12 months

  7. Non-relapse mortality

    Time frame: At 6 months

  8. Non-relapse mortality

    Time frame: At 12 months

  9. Incidence of toxicities

    CTCAE Grade \>1

    Time frame: Up to 45 days after transplantation

06

Study locations

No study locations are listed for this record.

07

References and documents

Individual participant data

Plan to share: Undecided

No publications or documents are linked to this record.

08

Registry details

Key details

Study ID
NCT07805746
Lead sponsor
Assistance Publique - Hôpitaux de Paris
Responsible party
Sponsor
First posted
Sep 4, 2026
Start date
Sep 15, 2026 (estimated)
Primary completion
Apr 1, 2028 (estimated)
Completion
Apr 1, 2029 (estimated)
Last update
Sep 4, 2026

Study contacts

Marie Robin, MD PhD
Contact
marie.robin@aphp.fr
01 42 49 47 60 ext. +33
Jérôme Lambert
Contact
jerome.lambert@u-paris.fr
0142499742

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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