An observational study in Gingivitis; Chronic, sponsored by Mustafa Kemal University. Not yet recruiting at 2 sites in Turkey (Türkiye). Open to participants aged 18 Years to 60 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-07-08.
Sponsored by Mustafa Kemal University · Observational
This pilot study aims to evaluate the effects of miswak use on the oral biological environment by comparing biochemical parameters obtained from saliva and dental plaque samples collected from individuals who use miswak and controls. A total of 36 healthy volunteers will be enrolled in the study, including 18 miswak+rutine oral hygiene products users and 18 rutine oral hygiene products.
Periodontal clinical parameters will be recorded, and saliva and supragingival dental plaque samples will be collected from all participants. The collected samples will be analysed for biomarkers associated with periodontal inflammation and oxidative stress. Interleukin-1 beta (IL-1β), C-reactive protein (CRP), and oxidative stress levels will be measured using biochemical analysis.
The findings of this study are expected to provide preliminary evidence regarding the biological effects of miswak on oral health and contribute to the current understanding of its potential role as an adjunctive oral hygiene tool.
631 studies on the registry are indexed under Gingivitis; 107 are open to participants now.
This study's planned enrollment of 36 is below the median of 93 across 116 observational studies indexed under Gingivitis.
Browse Gingivitis studies →Mustafa Kemal University is the lead sponsor of 77 studies on the registry; 18 are open to participants now.
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Patients diagnosed with gingivitis who attended the Department of Periodontology and met the eligibility criteria. Eligible participants will be systemic healthy patients with gingivitis who have not received periodontal treatment for 6 months. A total of 36 participants were enrolled and randomly allocated to the intervention and control groups.
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Exclusion Criteria:
Participants received oral hygiene instructions and used a manual toothbrush twice daily in combination with miswak (Salvadora persica L.) five times daily for one month before periodontal treatment. Clinical periodontal parameters will be recorded, and salivary IL-1β, CRP, and oxidative stress levels will be evaluated at pre-op (4 weeks), baseline (before periodontal treatment), and post-op (6 weeks).
Combination Product: Miswak · Other: Toothpaste
articipants with gingivitis received oral hygiene instructions and used a manual toothbrush twice daily without miswak. Clinical periodontal parameters will be recorded, and salivary IL-1β, CRP, and oxidative stress levels will be evaluated at pre-op (4 weeks), baseline (before periodontal treatment), and post-op (6 weeks).
Other: Toothpaste
Participants used miswak (Salvadora persica L.) as an adjunct to toothbrushing, five times per day, for one month before periodontal treatment.
Participants used a manual toothbrush twice daily for one month before periodontal treatment.
Change in Salivary Interleukin-1 Beta (IL-1β) Levels
To evaluate the effect of adjunctive miswak use on salivary interleukin-1 beta (IL-1β) levels in patients with gingivitis. Salivary IL-1β concentrations will be measured using enzyme-linked immunosorbent assay (ELISA).
Time frame: Pre-op 4 weeks and after 6 weeks
Change in Clinical Periodontal Indices
To evaluate changes in clinical periodontal parameters, including Plaque Index (PI), Gingival Index (GI), and Bleeding on Probing (BOP) following adjunctive miswak use.
Time frame: Pre-op 4 weeks and after 6 weeks
Change in Salivary C-Reactive Protein (CRP) Levels
To evaluate changes in salivary C-reactive protein (CRP) concentrations as a marker of oral inflammatory status following adjunctive miswak use.
Time frame: Pre-op 4 weeks and after 6 weeks
Change in Salivary Oxidative Stress Levels
To evaluate changes in salivary oxidative stress levels following adjunctive miswak use using appropriate biochemical assays.
Time frame: Pre-op 4 weeks and after 6 weeks
This study is not yet recruiting, as verified in Jul 2026. You cannot join it, but the record below documents what was studied.
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Mustafa Kemal University