An interventional study of Sulforaphane-rich Broccoli Sprout Extract and High Intensity Interval Treadmill Running in Immunometabolism, Mitochondrial Function, Bioenergetics and Inflammation, sponsored by University of British Columbia. Recruiting at 1 site in Canada. Open to participants aged 19 Years to 35 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-07-17.
Sponsored by University of British Columbia · Not applicable, Interventional, and Basic science
The study consist of 3 interventional periods which will be completed by each consenting participant in a randomized, crossover fashion. The interventional periods will be separated by a minimum 1 week washout period. One of the interventions consists of 4 days of daily supplementation with the commercially available broccoli sprout extract capsules (BrocElite). Another intervention will consist of 4 days of daily supervised treadmill running. The exercise protocol consists of 4 minutes of high-intensity running running followed by a 3 minute recovery period, repeated for a total of 4 repetitions. The last intervention combines the above two and consists of 4 days of daily supplementation and daily supervised exercise training. Before and after each intervention, basic anthropometrics (weight, heart rate, and blood pressure) will be recorded, and a venous blood sample will be collected. Blood samples will be analyzed for metabolic and inflammatory markers. Participants will be asked to refrain from structured exercise, alcohol consumption, and broccoli consumption during each of the experimental periods. Before any testing, willing participants will provide informed consent, and fill out a few demographic, and lifestyle questionnaires. The study will be conducted at the University of British Columbia in Kelowna, BC, Canada.
3,439 studies on the registry are indexed under Inflammation; 629 are open to participants now.
This study's planned enrollment of 20 is below the median of 50 across 2,437 interventional studies indexed under Inflammation.
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Of its 6 completed or terminated interventional studies of FDA-regulated products, 1 (17%) have results posted.
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Exclusion Criteria:
In this intervention, participants will consume commercially available broccoli sprout extract capsules daily for 4 days (20 mg/day) . Basic anthropometrics (weight, heart rate, blood pressure) and a venous blood sample (30 mL) will be collected before and after this interventional period.
Dietary Supplement: Sulforaphane-rich Broccoli Sprout Extract
In this intervention, participants will complete supervised exercise sessions daily for 4 days. The exercise intervention consists of interval running on a treadmill which contains 4 cycles of: 4 minutes of intense running (85-95% HRmax) followed by 3 minutes at a slow, easy pace. Basic anthropometrics (weight, heart rate, blood pressure) and a venous blood sample (30 mL) will be collected before and after this interventional period.
Other: High Intensity Interval Treadmill Running
In this intervention, participants will consume commercially available broccoli sprout extract tablets daily for 4 days. In addition, participants will complete supervised exercise sessions daily for 4 days. The exercise intervention consists of interval running on a treadmill which contains 4 cycles of: 4 minutes of intense running (85-95% HRmax) followed by 3 minutes at a slow, easy pace. Basic anthropometrics (weight, heart rate, blood pressure) and a venous blood sample (30 mL) will be collected before and after this interventional period.
Dietary Supplement: Sulforaphane-rich Broccoli Sprout Extract · Other: High Intensity Interval Treadmill Running
Broc Elite Plus stabilized sulforaphane capsules from Mara Labs
4 cycles of: 4 minutes of intense treadmill running (85-95% HR max) followed by 3 minutes of slow, easy running.
Change in peripheral blood mononuclear cell oxygen consumption rate
OCR will be measured using a Seahorse Analyzer before and after each 4-day intervention
Time frame: 0-4 days
TNF-alpha secretion from LPS-stimulated whole blood
TNF-alpha secretion from LPS-stimulated whole blood will be measured via ELISA before and after each 4-day period
Time frame: 0-4 days
Change in percentage of classical monocytes before and after the 6-week intervention
The percentage of classical monocytes will be measured using flow cytometry at baseline and after the 4-day intervention
Time frame: 0-4 days
Change in percentage of intermediate monocytes before and after the 4-day intervention
The percentage of intermediate monocytes will be measured using flow cytometry at baseline and after the 4-day intervention
Time frame: 0-4 days
Change in percentage of non-classical monocytes before and after the 4-day intervention
The percentage of non-classical monocytes will be measured using flow cytometry at baseline and after the 4-day intervention
Time frame: 0-4 days
Change in TLR-4 expression on classical monocytes before and after the 4-day intervention
TLR-4 expression on classical monocytes will be measured using flow cytometry at baseline and after the 4-day intervention.
Time frame: 0-4 days
Change in TLR-4 expression on intermediate monocytes before and after the 4-day intervention
TLR-4 expression on intermediate monocytes will be measured using flow cytometry at baseline and after the 4-day intervention.
Time frame: 0-4 days
Change in TLR-4 expression on non-classical monocytes before and after the 4-day intervention
TLR-4 expression on non-classical monocytes will be measured using flow cytometry at baseline and after the 4-day intervention
Time frame: 0-4 days
Change in white blood cell counts before and after the 4-day intervention
White blood cell counts will be measured using a hematology analyzer at baseline and after the 4-day intervention
Time frame: 0-4 days
TNF-alpha secretion from PolyIC-stimulated whole blood
TNF-alpha secretion from PolyIC-stimulated whole blood will be measured via ELISA before and after each 4-day period
Time frame: 0-4 days
Change in peripheral blood mononuclear cell extracellular acidification rate before and after the 4-day period
PBMC ECAR will be measured using a Seahorse Analyzer at baseline and after the 4-day intervention
Time frame: 0-4 days
Change in PBMC mitotracker green staining before and after the 4-day period
PBMC mitotracker green will be measured using flow cytometry before and after the 4-day intervention period
Time frame: 0-4 days
Change in PBMC mitoSOX red staining before and after the 4-day period
PBMC mitoSOX red will be measured using flow cytometry before and after the 4-day intervention period
Time frame: 0-4 days
Change in PBMC mitochondrial respiration before and after the 4-day intervention period
PBMC mitochondrial respiration will be measured using high-resolution respirometry before and after the 4-day period
Time frame: 0-4 days
Plan to share: No — IPD will not be shared to ensure the privacy and confidentiality of participants, in accordance with ethical guidelines and data protection regulations. Additionally, this was not included in the informed consent, which did not cover data sharing or address potential risks of re-identification.
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University of British Columbia