CClinicalTrials.gg
Not yet recruitingNCT07627256Updated Jun 4, 2026

Weight Loss Composition and Metabolic Adaptation During Hypocaloric Dieting

An interventional study of Hypocaloric Diet in Obesity, sponsored by Tel Aviv University. Not yet recruiting. Open to participants aged 18 Years and older, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-06-04.

Sponsored by Tel Aviv University · Not applicable, Interventional, and Treatment

Phase
Not applicable
Study type
Interventional
Enrollment
150
Allocation
Not applicable
Ages
18 Years and older
Sex
All
01

Study summary

Weight loss induced by hypocaloric dietary interventions is commonly accompanied by reductions in resting metabolic rate (RMR), a phenomenon often described as metabolic adaptation. The magnitude of this decline varies substantially between individuals and may depend not only on the amount of weight lost but also on the composition of weight loss, particularly the relative contributions of fat mass and fat-free mass.

This prospective interventional study will investigate whether changes in body composition during a hypocaloric dietary intervention are associated with changes in RMR and metabolic adaptation. Approximately 150 adults with overweight or obesity will participate in a structured dietary program designed to induce moderate energy restriction. Body composition and resting metabolic rate will be assessed before and after the intervention to evaluate associations between fat mass loss, fat-free mass loss, and metabolic responses to weight reduction.

Read the detailed description

Weight loss achieved through caloric restriction is consistently associated with reductions in resting metabolic rate (RMR). In many individuals, the decrease in energy expenditure exceeds what would be predicted from the loss of body mass or body composition alone, a phenomenon commonly described as metabolic adaptation or adaptive thermogenesis. This response has been proposed as one potential contributor to the difficulty many individuals experience in maintaining weight loss over time.

Fat-free mass is the primary determinant of resting energy expenditure because it includes metabolically active tissues such as skeletal muscle and organs. Consequently, the relative proportion of fat mass and fat-free mass lost during weight reduction may influence the magnitude of RMR decline. Dietary strategies that promote preservation of fat-free mass, such as adequate protein intake and engagement in physical activity, may attenuate metabolic slowing during weight loss. However, substantial inter-individual variability in metabolic responses to weight loss has been reported, and the role of weight-loss composition in shaping these responses remains incompletely understood.

The present study aims to examine the relationship between changes in body composition and changes in resting metabolic rate during a structured hypocaloric dietary intervention in adults with overweight or obesity. Participants will follow individualized dietary plans designed to produce a moderate energy deficit. Body composition will be assessed using dual-energy X-ray absorptiometry (DXA), and resting metabolic rate will be measured using indirect calorimetry at baseline and following completion of the intervention. Anthropometric measures and physical activity patterns will also be recorded.

By evaluating the relative contributions of fat mass loss and fat-free mass loss to changes in energy expenditure, this study aims to improve understanding of the physiological responses to weight reduction. The findings may help inform weight-loss strategies designed to minimize unfavorable metabolic adaptations and support more sustainable long-term weight management.

02

Conditions studied

  • Obesity

Keywords

  • Weight loss
  • Metabolic adaptation
  • Resting metabolic rate
  • Body composition
  • Fat-free mass
  • Energy expenditure
  • Hypocaloric diet
03

Who can participate

Ages eligible
18 Years and older
Sexes eligible
All
Accepts healthy volunteers
Yes

Inclusion criteria

  • Adults aged 18 years and older.
  • Individuals living with overweight or obesity.
  • Willingness and ability to comply with dietary recommendations and study procedures.
  • Provision of written informed consent.

Exclusion criteria

Exclusion Criteria

  • Diagnosed metabolic or endocrine disorders known to affect energy expenditure.
  • Use of medications known to substantially affect metabolism or body composition.
  • Pregnancy or breastfeeding.
  • Any medical condition that, in the opinion of the investigators, precludes safe participation.
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Study design

Phase
Not applicable
Primary purpose
Treatment
Allocation
Not applicable
Intervention model
Single group
Masking
None (open label)
Enrollment
150 participants (estimated)

Study arms

  • Experimental
    Hypocaloric Dietary Intervention

    Participants will follow individualized hypocaloric dietary plans designed to produce a moderate daily energy deficit based on measured resting metabolic rate. The intervention focuses on dietary energy restriction while supporting preservation of fat-free mass through adequate protein intake. Body composition and resting metabolic rate will be assessed before and after the intervention to evaluate metabolic responses to weight loss.

    Behavioral: Hypocaloric Diet

Interventions

  • BehavioralHypocaloric Diet

    Participants will follow individualized dietary plans designed to produce an energy deficit of approximately 500-600 kcal/day relative to measured resting metabolic rate. Protein intake will be prescribed at approximately 1.5 g/kg body weight per day to support preservation of fat-free mass. Dietary plans will be adjusted as needed during the intervention period, and participants will attend regular follow-up visits to monitor progress and adherence.

05

What researchers measure

Primary outcomes

  1. Change in Resting Metabolic Rate

    Change in resting metabolic rate (RMR) measured by indirect calorimetry between baseline and post-intervention assessment.

    Time frame: Baseline to end of dietary intervention (up to 12 months)

Secondary outcomes

  1. Change in Fat Mass

    Change in total body fat mass measured using dual-energy X-ray absorptiometry (DXA).

    Time frame: Baseline to end of dietary intervention (up to 12 months)

  2. Change in Fat-Free Mass

    Change in total body fat-free mass measured using dual-energy X-ray absorptiometry (DXA).

    Time frame: Baseline to end of dietary intervention (up to 12 months)

  3. Change in Body Weight

    Change in body weight measured using a calibrated digital scale.

    Time frame: Baseline to end of dietary intervention (up to 12 months)

  4. Change in Abdominal Circumference

    Change in abdominal circumference measured using a standardized anthropometric tape at the midpoint between the lowest rib and iliac crest.

    Time frame: Baseline to end of dietary intervention (up to 12 months)

  5. Intervention Duration

    Total duration of participation in the hypocaloric dietary intervention, recorded in weeks from baseline to intervention completion.

    Time frame: Baseline to end of intervention (up to 12 months)

  6. Physical Activity Modality

    Self-reported type of physical activity performed during the intervention, categorized as aerobic, resistance, combined, or none.

    Time frame: Baseline to end of dietary intervention (up to 12 months)

06

Study locations

No study locations are listed for this record.

07

References and documents

Individual participant data

Plan to share: No

No publications or documents are linked to this record.

08

Registry details

Key details

Study ID
NCT07627256
Lead sponsor
Tel Aviv University
Responsible party
Gepner Yftach (Principal Investigator - Professor, Tel Aviv University) — Principal investigator
First posted
Jun 4, 2026
Start date
Jun 2026 (estimated)
Primary completion
Dec 2027 (estimated)
Completion
Dec 2027 (estimated)
Last update
Jun 4, 2026

Study contacts

Yftach Prof. Gepner, PhD
Contact
gepner@tauex.tau.ac.il
+972 50-682-8501
Lien Bruck
Contact
lienbruck@tauex.tau.ac.il
+972 587487431
Yftach Gepner, PhD
principal investigator · Tel Aviv University

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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