An interventional study of Sleep restriction in Sleep and Sleep Restriction, sponsored by University School of Physical Education, Krakow, Poland. Recruiting at 2 sites in Poland. Open to female participants aged 16 Years to 30 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2024-04-11.
Sponsored by University School of Physical Education, Krakow, Poland · Not applicable, Interventional, and Diagnostic
This research aims to investigate the interplay between menstrual cycles, sleep patterns, and athletic performance. Before experimental sessions, participants will maintain menstrual diaries for three months and complete assessments for sleep disorders and chronotype. Sleep duration will be objectively measured over five nights using actigraphy, complemented by sleep-related data from an actigraph and the Karolinska Sleep Diary.
The study involves two sessions: normal sleep (based on habitual duration) and restricted sleep (reduced by 3 hours). Athletes will engage in a simulated match-play game before both sessions, followed by comprehensive performance tests the next day. Blood samples will be collected at three intervals to analyze hormonal profiles (including progesterone, and estradiol), markers of muscle damage, inflammation, stress (such as TNF-α, IL-6, CRP, myoglobin, cortisol, testosterone), and brain-derived neurotrophic factor (BDNF).
On Day 1, pre- and post-training blood samples will be obtained, and participants will wear an actigraph, adhere to specified bedtime routines, and report activities (restricted sleep group). Day 2 involves completing a sleep diary, refraining from caffeine, responding to questionnaires assessing readiness to train and mood, and undergoing performance tests followed by self-reporting of pain levels and perceived exertion using established scales (VAS, RPE 6-20).
Before commencing experimental sessions, the menstrual cycles of the participants will be assessed using a menstrual diary spanning three months before the sessions. Additionally, each athlete will be asked to complete a questionnaire for screening sleep disorders (PSQI) and a chronotype classification questionnaire (MEQ). The average sleep duration over five consecutive nights will be measured using actigraphy; this analysis will determine the length of sleep allocated during experimental sessions. Sleep-related parameters will be recorded using an actigraph (MotionWatch8) and confirmed using the Karolinska Sleep Diary (KSD).
The project involves two experimental sessions (each spanning two days) under two different conditions: a) normal sleep (NS; habitual sleep duration determined from the analysis of five consecutive nights) and b) restricted sleep (RS; sleep reduced by 3 hours during the nocturnal period compared to NS). Participants will perform a simulation match-play game (SAFT90) before NS and RS, followed by performance tests on the following day. Blood samples will be collected at three-time points: a) pre-training, b) post-training, and c) before morning tests on the second day of the experiment. Biochemical analysis of the blood will include progesterone and estradiol (for menstrual cycle analysis) as well as parameters related to muscle damage, inflammatory state, and stress levels (TNF-α; IL-6; CRP; myoglobin; hematology; cortisol; testosterone). Additionally, the level of brain-derived neurotrophic factor (BDNF) will be analyzed.
Day 1 of the experiment: Immediately before training, the first blood collection (8 ml) will occur to analyze the necessary blood biochemical parameters. Subsequently, athletes will undergo a standardized afternoon training session, including a simulation match-play game (SAFT90). Another blood sample will be taken immediately after the exercise. Participants will be asked to wear an actigraph on their non-dominant wrist and consume dinner according to the provided recommendations. They will be instructed to go to bed according to a specified schedule (NS or RS). Additionally, participants will be instructed to spend the time before bedtime engaged in routine evening activities (excluding stimulants like coffee or additional exercise). Participants with restricted sleep will also be requested to report their activities every 30 minutes until they go to bed using an internet communicator.
Day 2 of the experiment: Participants will complete the Karolinska Sleep Diary upon waking up. They will also be asked to abstain from consuming caffeine-containing foods and to have breakfast following the provided recommendations. Before the session begins, participants will complete a readiness to train questionnaire (RTQ) and a mood assessment questionnaire (POMS). Blood samples will be collected, followed by specific football-related performance tests: a) repeated high-intensity effort capacity (Yo-Yo test); b) lower limb power (vertical jump); c) maximal acceleration (3x30 m sprints); d) agility with and without the ball (Zig-Zag Test). Immediately after the performance tests, participants will indicate their pain level (Visual Analogue Scale; VAS) and rate their perceived exertion (RPE 6-20).
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This study's planned enrollment of 20 is below the median of 50 across 2,437 interventional studies indexed under Inflammation.
Browse Inflammation studies →University School of Physical Education, Krakow, Poland is the lead sponsor of 20 studies on the registry; 8 are open to participants now.
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Exclusion Criteria:
The night before the experimental session, where participants will follow their habitual sleep-wake routines.
The night before the experimental session (with sleep restrictions (SR)), where participants will experience acute sleep restriction the night before the test session (i.e., 3 h of early sleep restriction versus normal sleep).
Procedure: Sleep restriction
Participants will experience a night of acute sleep restriction. During this night, they will sleep 3 hours less than usual.
Countermovement jump
Vertical jump test performed by having an athlete quickly squat to a self-selected depth and then jump as high as possible.
Time frame: 1 day after night with or without intervention
Sprint test (0-30m)
Time frame: 1 day after night with or without intervention
Zig Zag test
Time frame: 1 day after night with or without intervention
Zig Zag test with ball
Time frame: 1 day after night with or without intervention
Yo-Yo Intermittent endurance test level I
Time frame: 1 day after night with or without intervention
Flanker task
Time frame: 1 day after night with or without intervention
Mood (POMS)
The POMS is a widely-applied measure for the assessment of an individual's mood.
Time frame: 1 day after night with or without intervention. During each experimental session. Before sports performance protocol
Pain (Pain scale)
A pain scale is a tool use to help assess a person's pain. Scale 0 (lack of pain) 10 (maximum pain)
Time frame: 1 day after night with or without intervention. Immediately after the last performance exercise in experimental sessions
Readiness-to-train questionnaire (RTT-Q)
Subjective reactions related to the level of motivation for effort
Time frame: 1 day after night with or without intervention. During each experimental session. Before sports performance protocol
Rating perceived exertion (RPE)
A subjective scale that assesses the severity of effort exerted. Includes a rating from 6 perceiving "no effort" to 20 perceiving "maximum effort."
Time frame: 1 day after night with or without intervention. Immediately after the last performance exercise in experimental sessions
Stress and recovery (SRSS)
The Short Recovery and Stress Scale (SRSS) measure the recovery-stress state of an athlete multidimensionally with eight items on emotional, mental, physical, and overall levels.
Time frame: 1 day after night with or without intervention. In the morning, each experimental day and the day after
Objective Sleep - Total sleep time
Total sleep time obtained from sleep onset to time at wake-up assessed by actigraphy
Time frame: 1 day after night with or without intervention. The night before and after the experimental sessions
Objective Sleep - Sleep efficiency
The percentage of total sleep time to lights off and leaving bed assessed by actigraphy
Time frame: 1 day after night with or without intervention. The night before and after the experimental sessions
Objective Sleep - WASO (Wake After Sleep Onset)
The total number of minutes that a person is awake after having initially fallen asleep assessed by actigraphy
Time frame: 1 day after night with or without intervention. The night before and after the experimental sessions
Objective Sleep - Sleep duration
The quantity of time that a person sleeps assessed by actigraphy
Time frame: 1 day after night with or without intervention. The night before and after the experimental sessions
Objective Sleep - Sleep onset time
The time it takes a person to fall asleep after turning the lights out assessed by actigraphy
Time frame: 1 day after night with or without intervention. The night before and after the experimental sessions
Objective Sleep - Wake time
The hours in the day that a person is awake assessed by actigraphy
Time frame: 1 day after night with or without intervention. The night before and after the experimental sessions
Blood collection - progesterone
Blood will be drawn to measure progesterone
Time frame: 1 day after night with or without intervention. Before each experimental sesion
Blood collection - estradiol
Blood will be drawn to measure estradiol
Time frame: 1 day after night with or without intervention. Before each experimental sesion
Blood collection - cortisol
Time frame: Before and after the match simulation and before the experimental session on the second day 1 day after night with or without intervention.
Blood collection - testosterone
Time frame: 1 day after night with or without intervention. Before and after the match simulation and before the experimental session on the second day
Blood collection - TNF-α
Time frame: 1 day after night with or without intervention. Before and after the match simulation and before the experimental session on the second day
Blood collection - IL-6
Time frame: 1 day after night with or without intervention. Before and after the match simulation and before the experimental session on the second day
Blood collection - CRP
Time frame: 1 day after night with or without intervention. Before and after the match simulation and before the experimental session on the second day
Blood collection - myoglobin
Time frame: 1 day after night with or without intervention. Before and after the match simulation and before the experimental session on the second day
Blood collection - brain-derived neurotrophic factor (BDNF)
Time frame: 1 day after night with or without intervention. Before and after the match simulation and before the experimental session on the second day
Karolinska Sleep Diary (KSD)
Time frame: 1 day after night with or without intervention. The morning of the session and the next day after the experimental session
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University School of Physical Education, Krakow, Poland