An interventional study of Exercise tests (VO2 max test and 45-minute exercise session at 65% VO2 max) in Normal-weight Obesity and Obesity, sponsored by Oklahoma State University. Recruiting at 1 site in United States. Open to participants aged 18 Years to 50 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2023-06-05.
Sponsored by Oklahoma State University · Not applicable, Interventional, and Prevention
Exercise acutely increases gut permeability and inflammation, even in healthy populations. However, whether this response differs in groups at-risk for CVD that present with low-grade inflammation (e.g., normal-weight obesity) has yet to be examined. The investigators aim to measure serum indicators of gut permeability in those with normal-weight obesity pre- and post-short, intense exercise and sustained, moderate exercise
The investigator's overall goal is to examine how indicators of gut permeability change in response to exercise in individuals with normal-weight obesity (i.e., normal body mass index [BMI] and high body fat percent) relative to those with a normal BMI and low body fat percent and those with a BMI in the obesity range and high body fat percent.
The investigators will examine indicators of gut permeability (specified in primary outcomes) in response to a short, intense bout of exercise (i.e., VO2 max test on a cycle ergometer) and sustained, moderate bout of exercise (i.e., 45 minutes at 65% measured VO2 max on a cycle ergometer).
Additionally, the investigators will measure basic anthropometrics, blood lipids and glucose, and body composition with DXA.
Control group: normal BMI, body fat percentage \< 25% (male) or \< 35% (female)
Normal-weight obesity: normal BMI, body fat percentage > 25% (male) or > 35% (female)
Overt obesity: BMI in obesity range, body fat percentage > 25% (male) or > 35% (female)
Exclusion Criteria:
The low risk comparator group for this study will consist of individuals with normal BMI (18.5 - 24.9 kg/m2) and body fat percent \< 25% (male) or \< 35% (female).
Other: Exercise tests (VO2 max test and 45-minute exercise session at 65% VO2 max)
Individuals with normal-weight obesity will be defined as having normal BMI (18.5 - 24.9 kg/m2), body fat percent \> 25% (male) or \> 35% (female).
Other: Exercise tests (VO2 max test and 45-minute exercise session at 65% VO2 max)
Overt obesity (BMI \> 30 kg/m2) with high body fat percent (\> 25% \[male\] or \> 35% \[female\]) will be used as a high-risk comparator group.
Other: Exercise tests (VO2 max test and 45-minute exercise session at 65% VO2 max)
All participants will complete two exercise sessions: 1. VO2 max test on a cycle ergometer 2. Moderate exercise session (45 minutes at 65% measured VO2 max)
Serum concentrations of FABP2 following short, intense exercise
The investigators will examine peak and absolute change in this indicator of gut permeability after the VO2 max test on a cycle ergometer.
Time frame: Approximately 8 months
Serum concentrations of LBP following short, intense exercise
The investigators will examine peak and absolute change in this indicator of gut permeability after the VO2 max test on a cycle ergometer.
Time frame: Approximately 8 months
Serum concentrations of sCD14 following short, intense exercise
The investigators will examine peak and absolute change in this indicator of gut permeability after the VO2 max test on a cycle ergometer.
Time frame: Approximately 8 months
Serum concentrations of FABP2 following sustained, moderate exercise
The investigators will examine peak and absolute change in this indicator of gut permeability after 45-minutes of exercise at 65% VO2 max on a cycle ergometer.
Time frame: Approximately 8 months
Serum concentrations of IL-6 following sustained, moderate exercise
The investigators will examine peak and absolute change in this inflammatory marker after 45-minutes of exercise at 65% VO2 max on a cycle ergometer.
Time frame: Approximately 8 months
VO2 Max
The investigators will compare VO2 max after the short, intense exercise session across study groups.
Time frame: Approximately 8 months
Respiratory exchange ratio (RER) during VO2 max test
The investigators will compare RER dynamics during the short, intense exercise session across study groups.
Time frame: Approximately 8 months
Maximum workload during exercise sessions
The investigators will compare workload dynamics in watts during the short, intense and moderate, sustained exercise session across study groups.
Time frame: Approximately 8 months
Heart rate dynamics during exercise sessions
The investigators will compare heart rate dynamics during the short, intense and moderate, sustained exercise session across study groups.
Time frame: Approximately 8 months
Body fat percent assessment with dual-energy X-ray absorptiometry (DXA).
The investigators will perform DXA scans on all participants to assess body fat percent.
Time frame: Approximately 8 months
Absolute body fat assessment with dual-energy X-ray absorptiometry (DXA)
The investigators will perform DXA scans on all participants to assess absolute body fat in kilograms.
Time frame: Approximately 8 months
Lean mass percent assessment with dual-energy X-ray absorptiometry (DXA)
The investigators will perform DXA scans on all participants to assess lean mass percent.
Time frame: Approximately 8 months
Absolute lean mass assessment with dual-energy X-ray absorptiometry (DXA)
The investigators will perform DXA scans on all participants to assess absolute lean mass in kilograms.
Time frame: Approximately 8 months
Absolute visceral fat mass assessment with dual-energy X-ray absorptiometry (DXA)
The investigators will perform DXA scans on all participants to assess absolute visceral mass in grams.
Time frame: Approximately 8 months
Lipid panel with Abbott Piccolo Xpress Clinical Chemistry Analyzer
The investigators will perform a lipid panel on all participants.
Time frame: Approximately 8 months
Plan to share: Undecided
No publications or documents are linked to this record.
Eligibility is decided by the study team. Share this record with your doctor or contact the team directly.
Contact study teamGet an email when the registry record changes — status, dates, results — or when someone posts here.
Sign in to followQuestions and observations about this study, from anyone following it. Not medical advice, and not a channel to the study team — their contact details are on the registry record.
Sign in to join the discussion. Reading takes no account; posting does. You choose a display name, and a pseudonym is the default.
Nothing here yet. If you are running this trial, taking part in it, or weighing whether to, this is the place to say so.
Oklahoma State University