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RecruitingNCT05877599Updated Sep 29, 2026

A Study of NT-175 in Adult Participants With Advanced Malignancies That Are Positive for HLA-A*02:01 and the TP53 R175H Mutation

A Phase 1 interventional study of NT-175 in Non-small Cell Lung Cancer, Head and Neck Squamous Cell Carcinoma and Colorectal Carcinoma, sponsored by AstraZeneca. Recruiting at 20 sites in United States. Open to participants aged 18 Years and older. Per ClinicalTrials.gov, last updated 2026-09-29.

Sponsored by AstraZeneca · Phase 1, Interventional, and Treatment

Phase
Phase 1
Study type
Interventional
Enrollment
45
Allocation
Non-randomized
Ages
18 Years and older
Sex
All
01

Study summary

Phase I Study of NT-175, an autologous T cell therapy product genetically engineered to express an HLA-A*02:01-restricted T cell receptor (TCR), targeting TP53 R175H mutant malignancies

Read the detailed description

This is a Phase 1, open-label, multicentre platform study to evaluate the safety and preliminary antitumour activity of NT-175 in HLA-A*02:01 participants with advanced malignancies that are positive for the TP53 R175H mutation.

Dose Escalation will investigate escalating doses of NT-175 in adult subjects with eligible histologies and will evaluate the safety and MTD and/or RDE/RP2D.

Cohort expansion will further evaluate the safety and preliminary anti-tumour activity at or below the MTD in disease specific histologies and determine the RP2D.

Dose Expansion will further evaluate the preliminary anti-tumour activity and safety of NT-175 at the RP2D in disease specific settings.

02

Conditions studied

  • Non-small Cell Lung Cancer
  • Head and Neck Squamous Cell Carcinoma
  • Colorectal Carcinoma
  • Pancreatic Adenocarcinoma
  • Breast Cancer
  • Other Solid Tumors
  • Ovarian Cancer
  • Myeloid Neoplasms (AML/MDS)

Keywords

  • Cell therapy
  • TP53
  • Solid tumors
  • Non-small cell lung cancer
  • Head and neck squamous cell carcinoma
  • Colorectal carcinoma
  • Pancreatic adenocarcinoma
  • Breast Cancer
  • Ovarian Cancer
  • AML
  • MDS
  • TCR
03

Who can participate

Ages eligible
18 Years and older
Sexes eligible
All
Accepts healthy volunteers
No

Eligibility criteria

Key Inclusion Criteria (Module 1)

  • Subjects must be at least 18 years of age
  • Subject must be diagnosed with one of the histologies below:

    • NSCLC
    • Colorectal adenocarcinoma
    • HNSCC
    • Pancreatic adenocarcinoma
    • Breast cancer
    • Ovarian cancer
    • Any other solid tumor
  • Tumors must harbor a TP53 R175H variant mutation and subject must be HLA-A*02:01 positive (at least 1 allele)
  • Subject has advanced solid cancer, defined as unresectable, advanced, and/or metastatic disease (Stage III or IV) after at least 1 line of approved systemic standard of care (SOC) treatment regimen and for which there are no available curative treatment options.
  • Subject has at least 1 measurable lesion
  • Eastern Cooperative Oncology Group (ECOG) performance status of 0 to 1
  • Adequate hematological, renal, hepatic, pulmonary, and cardiac function

Key Exclusion Criteria (Module 1)

  • Any another primary malignancy within the 3 years prior to enrollment
  • Known, active primary central nervous system (CNS) malignancy
  • History of prior adoptive cell and gene therapy, allogeneic stem cell transplant or solid organ transplantation.
  • History of clinically significant cardiac disease within the 6 months prior to enrollment or heart failure at any time prior to enrollment.
  • Systemic therapy within at least 2 weeks or 3 half-lives, whichever is shorter, prior to enrollment.
  • Any form of primary immunodeficiency.
  • Known to have Li-Fraumeni syndrome or is known to have relatives who are diagnosed with Li-Fraumeni syndrome.

Key Inclusion Criteria (Module 2 - hematological malignancies)

  • At least 18 years of age
  • Diagnosis of AML or MDS that allows for efficacy assessments
  • Confirmation of TP53 R175H variant mutation in cancer cells
  • Subject must be HLA-A*02:01 positive (at least 1 allele)
  • ECOG performance status of 0 to 1

Key Exclusion Criteria (Module 2 - hematological malignancy)

  • Acute promyelocytic leukaemia or isolated extramedullary disease
  • Another primary malignancy within 2 years (with exceptions)
  • HSCT within 100 days or immunosuppression for GvHD within 4 weeks
  • History of CNS or other extramedullary leukaemic involvement unless a lumbar puncture is negative for leukemic cells
  • Prior stroke, ischemic attack, significant cardiac disease, heart failure
  • Prior adoptive modified cell therapy
  • Known to have Li-Fraumeni syndrome or is known to have relatives who are diagnosed with Li-Fraumeni syndrome.
04

Study design

Phase
Phase 1
Primary purpose
Treatment
Allocation
Non-randomized
Intervention model
Single group
Masking
None (open label)
Enrollment
45 participants (estimated)

Study arms

  • Experimental
    NT-175 for advanced malignancies

    TCR T cell therapy product

    Biological: NT-175

Interventions

  • BiologicalNT-175

    * Pre-conditioning by non-myeloablative chemotherapy with fludarabine and cyclophosphamide * Single infusion Autologous, engineered T Cells targeting TP53 R175H * Post-infusion recombinant interleukin-2 (rIL-2)

05

What researchers measure

Primary outcomes

  1. Module 1, Part 1: Safety of NT-175 in participants with unresectable, advanced, and/or metastatic solid tumours

    Incidence of dose-limiting toxicities (DLTs) after the infusion of NT-175

    Time frame: 28 days after infusion

  2. Module 1, Part 1: Safety of NT-175 in participants with unresectable, advanced, and/or metastatic solid tumours

    Incidence of Treatment Emergent Adverse Events (TEAE) Serious Adverse Events (SAE)

    Time frame: Up to 24 months post-infusion

  3. Module 1, Part 2: Preliminary anti-tumour activity of NT-175 in participants with unresectable, advanced, and/or metastatic solid tumours

    Per RECIST v1.1 determined by Investigator assessment: * Objective Response Rate (ORR) * Best Overall Response (BOR) * Duration of Response (DOR) * Clinical Benefit Rate (CBR) * Time to Response (TTR) * Progression-free survival (PFS) * Overall Survival (OS)

    Time frame: Up to 24 months after infusion

  4. Module 2: Safety of NT-175 in participants with haematological malignancies

    \- Incidence of dose-limiting toxicities (DLTs) after the infusion of NT-175

    Time frame: Up to 28 days after infusion

  5. Module 2: Safety of NT-175 in participants with haematological malignancies

    * Incidence of Treatment Emergent Adverse Events (TEAE) * Serious Adverse Events (SAE)

    Time frame: Up to 24 months after infusion

Secondary outcomes

  1. Module 1, Part 1: Preliminary anti-tumor activity of NT-175 in participants with unresectable, advanced, and/or metastatic solid tumours

    Per RECIST v1.1 determined by Investigator assessment: * Objective Response Rate (ORR) * Best Overall Response (BOR) * Duration of Response (DOR) * Clinical Benefit Rate (CBR) * Time to Response (TTR) * Progression-free survival (PFS) * Overall Survival (OS)

    Time frame: Up to 24 months after infusion

  2. Module 2: Evaluate preliminary anti-tumour activity in participants with AML or MDS

    Per ELN 2022 criteria for AML and per IWG 2023 criteria for MDS by Investigator assessment: * Objective Response Rate (ORR) * Time to Response (TTR) * Duration of Response (DOR) * Event-free survival (EFS) By Investigator assessment: * Transfusion Independence (TI) * Overall Survival (OS) * Complete Response (CR) + Complete response with partial haematological recover (CRh) * Complete Response with limited count recovery (CRL) (CRuni + CRbi) in MDS * MDS time to Progression to AML * Proportion of participants with subsequent Haematopoietic Stem Cell Transplantation (HSCT)

    Time frame: Up to 24 months after infusion

06

Study locations

16 of 20 sites recruiting
  • Research Site
    Gilbert, Arizona 85234, United States
    Recruiting
  • Research Site
    Duarte, California 91010, United States
    Recruiting
  • Research Site
    Los Angeles, California 90095, United States
    Recruiting
  • Research Site
    Newport Beach, California 92663, United States
    Recruiting
  • Research Site
    Santa Monica, California 90404, United States
    Recruiting
  • Research Site
    Jacksonville, Florida 32224, United States
    Recruiting
  • Research Site
    Miami, Florida 33136, United States
    Withdrawn
  • Research Site
    Tampa, Florida 33612, United States
    Withdrawn
  • Research Site
    Boston, Massachusetts 02115, United States
    Recruiting
  • Research Site
    Boston, Massachusetts 02215, United States
    Recruiting
  • Research Site
    New Brunswick, New Jersey 08901, United States
    Recruiting
  • Research Site
    New York, New York 10065, United States
    Recruiting
  • Research Site
    Charlotte, North Carolina 28204, United States
    Withdrawn
  • Research Site
    Winston-Salem, North Carolina 27103, United States
    Withdrawn
  • Research Site
    Portland, Oregon 97213, United States
    Recruiting
  • Research Site
    Pittsburgh, Pennsylvania 15232, United States
    Recruiting
  • Research Site
    Nashville, Tennessee 37203, United States
    Recruiting
  • Research Site
    Houston, Texas 77030, United States
    Recruiting
  • Research Site
    Round Rock, Texas 78665, United States
    Recruiting
  • Research Site
    Milwaukee, Wisconsin 53226, United States
    Recruiting
07

References and documents

Individual participant data

Plan to share: Yes — Qualified researchers can request access to anonymized individual patient-level data from AstraZeneca group of companies sponsored clinical trials via the request portal Vivli.org. All requests will be evaluated as per the AZ disclosure commitment: https://astrazenecagrouptrials.pharmacm.com/ST/Submission/Disclosure. Yes, indicates that AZ are accepting requests for IPD, but this does not mean all requests will be shared.

No publications or documents are linked to this record.

08

Registry details

Key details

Study ID
NCT05877599
Lead sponsor
AstraZeneca
Responsible party
Sponsor
First posted
May 26, 2023
Start date
Jul 12, 2023
Primary completion
Jul 31, 2029 (estimated)
Completion
Jul 31, 2029 (estimated)
Last update
Sep 29, 2026

Study contacts

AstraZeneca Clinical Study Information Center
Contact
information.center@astrazeneca.com
1-877-240-9479
AstraZeneca
study director · AstraZeneca

Oversight

Data monitoring committee
No
FDA-regulated drug
Yes
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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