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Not yet recruitingNCT07845916QS-NSCLCUpdated Sep 29, 2026

Quad Shot Radiotherapy Combined With Tislelizumab and Platinum-Based Chemotherapy Before Surgery for Stage II-III Non-Small Cell Lung Cancer

A Phase 2 interventional study of Quad Shot Radiotherapy and Tislelizumab in Non-Small Cell Lung Cancer, sponsored by Union Hospital, Tongji Medical College, Huazhong University of Science and Technology. Not yet recruiting at 1 site in China. Open to participants aged 18 Years to 75 Years. Per ClinicalTrials.gov, last updated 2026-09-29.

Sponsored by Union Hospital, Tongji Medical College, Huazhong University of Science and Technology · Phase 2, Interventional, and Treatment

Phase
Phase 2
Study type
Interventional
Enrollment
12
Allocation
Not applicable
Ages
18 Years to 75 Years
Sex
All
01

Study summary

This study aims to evaluate a new pre-surgery (neoadjuvant) treatment for patients with stage II-III non-small cell lung cancer (NSCLC). Currently, standard pre-surgery treatment involves immunotherapy combined with chemotherapy. While effective, many patients still have residual tumor cells after this standard treatment.

Radiation therapy can not only kill tumor cells but also help the immune system recognize and fight cancer. The type of radiation used in this study is called "Quad Shot" (QS) radiotherapy. It is a short, high-dose, cyclical treatment designed to boost the effects of immunotherapy with less toxicity than standard daily radiation.

Patients in this study will receive 3 cycles of QS radiotherapy combined with Tislelizumab (an immunotherapy drug) and standard platinum-based chemotherapy before surgery. The main goal of this study is to see if this combined approach can make the tumor completely disappear under a microscope (called pathological complete response, or pCR). Researchers will also monitor the safety of the treatment, side effects, and how well patients tolerate the treatment before undergoing surgery. Approximately 12 patients will be enrolled in this study.

Read the detailed description

Background:

Neoadjuvant immune checkpoint inhibitor (ICI) combined with platinum-based chemotherapy has become a standard of care for resectable stage II-III NSCLC, as demonstrated by phase III trials such as CheckMate-816, KEYNOTE-671, and RATIONALE-315. However, a significant proportion of patients still fail to achieve deep pathological responses due to mechanisms such as poor antigen release, impaired antigen presentation, and an immunosuppressive tumor microenvironment (TME).

Radiotherapy possesses immunomodulatory properties, inducing immunogenic cell death and promoting antigen release. However, traditional continuous fractionated radiotherapy may deplete circulating lymphocytes and cause normal tissue damage. The "Quad Shot" (QS) regimen is a cyclical hypofractionated radiotherapy approach (14-14.8 Gy in 4 fractions over 2 days) that has shown low toxicity in palliative settings. Its cyclical, pulsatile nature may provide repeated immune stimulation and TME remodeling, making it a promising partner for immunotherapy.

Study Design:

This is a single-arm, prospective, exploratory phase II clinical study. Patients with histologically or cytologically confirmed, untreated, resectable stage II-IIIB NSCLC (including T3-4N2 IIIB, AJCC 9th edition) will be enrolled.

Patients will receive 3 cycles of neoadjuvant treatment (every 3 weeks):

Quad Shot radiotherapy (14-14.8 Gy/4 fractions, Day 1-2) Tislelizumab (200 mg, Day 3) Platinum-based doublet chemotherapy (Day 3): Carboplatin (AUC 5) or Cisplatin (75 mg/m²) combined with Pemetrexed (500 mg/m², for non-squamous) or Paclitaxel (175 mg/m², for squamous).

Following 3 cycles of neoadjuvant therapy, patients will undergo surgery 4-6 weeks (±7 days) after the last dose. Postoperative adjuvant therapy will be administered at the investigator's discretion according to NCCN and CSCO guidelines.

Objectives and Endpoints:

The primary objective is to evaluate the antitumor efficacy of this regimen, with the primary endpoint being the pathological complete response (pCR) rate. Secondary endpoints include major pathological response (MPR) rate, R0 resection rate, objective response rate (ORR) per RECIST v1.1, event-free survival (EFS), overall survival (OS), safety (NCI CTCAE v5.0), feasibility, surgical complications (Clavien-Dindo classification), and QS radiotherapy-related toxicities.

Sample Size:

A total of 12 patients will be enrolled, with at least 10 evaluable patients included in the primary endpoint analysis to account for an estimated 2 dropouts.

Study Duration:

The estimated enrollment period is 6-12 months, with an anticipated study completion date of December 2031.

02

Conditions studied

  • Non-Small Cell Lung Cancer

Keywords

  • NSCLC
  • Neoadjuvant Therapy
  • Quad Shot Radiotherapy
  • Tislelizumab
  • Platinum-based Chemotherapy
  • Immunotherapy
  • Pathological Complete Response
03

Who can participate

Ages eligible
18 Years to 75 Years
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  1. Age 18-75 years, either gender.
  2. Histologically or cytologically confirmed, untreated stage II-IIIB non-small cell lung cancer (NSCLC) (including T3-4N2 IIIB, AJCC 9th edition). Baseline staging must include PET/CT (or chest + upper abdomen contrast-enhanced CT + whole body bone scan) + brain MRI. Stage III patients will be further subdivided into IIIA and IIIB per AJCC 9th edition for separate recording, evaluation, and analysis.
  3. Evaluated as resectable by a multidisciplinary team including thoracic surgeons.
  4. At least one evaluable target lesion per RECIST v1.1.
  5. ECOG Performance Status 0-1.
  6. Adequate major organ function: Neutrophils ≥ 1.5×10\^9/L; Platelets ≥ 100×10\^9/L; Hemoglobin > 9.0 g/dL; Serum creatinine ≤ 1.5×ULN or Creatinine Clearance (CrCl) ≥ 40 mL/min; AST/ALT ≤ 3×ULN; Total bilirubin ≤ 1.5×ULN; INR/APTT within normal range; FEV1 ≥ 1.2L or > 40% predicted.
  7. Signed written informed consent prior to any study-related procedures.
  8. Investigator judges that the patient is able to comply with the study protocol, willing and able to follow visit schedules, treatment plans, laboratory tests, and other study procedures.
  9. Negative pregnancy test at screening (for women of childbearing potential). Subjects of childbearing potential (male and female) must agree to use effective contraception from screening until 180 days after the last dose.

Exclusion criteria

Exclusion Criteria:

  1. Known or suspected autoimmune disease (except vitiligo, Type I diabetes, or hypothyroidism requiring only hormone replacement therapy with no signs of recurrence).
  2. Requires systemic corticosteroid therapy (>10 mg/day prednisolone or equivalent) or other immunosuppressive drugs within 14 days prior to enrollment (inhaled or topical steroids, and physiological replacement doses of adrenal corticosteroids are allowed).
  3. History of thoracic radiotherapy.
  4. Active bleeding before treatment.
  5. Severe heart, lung, liver, or kidney dysfunction, hematopoietic system disease, cachexia, or other conditions that make the patient unable to tolerate chemoradiotherapy.
  6. History of diabetes for more than 10 years with unsatisfactory blood glucose control.
  7. History of interstitial lung disease or non-infectious pneumonitis.
  8. NSCLC patients with EGFR sensitizing mutations or ALK fusion genes.
  9. Prior or concurrent other malignancies (except cured non-melanoma skin cancer and carcinoma in situ; or malignancies in complete remission for ≥ 2 years without requiring additional anti-tumor therapy during the study period).
  10. Prior treatment with anti-PD-1, anti-PD-L1, anti-PD-L2, anti-CTLA-4, or other drugs targeting T-cell costimulatory or immune regulatory pathways.
  11. Active hepatitis B (HBV DNA ≥ 2000 IU/mL or 10\^4 copies/mL) or hepatitis C (HCV antibody positive and HCV-RNA above the lower limit of detection).
  12. HIV positive or diagnosed with acquired immunodeficiency syndrome (AIDS).
  13. Received live vaccine within 4 weeks prior to the start of study treatment.
  14. Known or suspected allergy to the study drugs or any related drugs in this trial.
  15. Pregnant or breastfeeding women.
  16. Investigator considers that the patient has other factors that may prevent completion of the study or affect subject safety or data collection.
04

Study design

Phase
Phase 2
Primary purpose
Treatment
Allocation
Not applicable
Intervention model
Single group
Masking
None (open label)
Enrollment
12 participants (estimated)

Study arms

  • Experimental
    Neoadjuvant Quad Shot Radiotherapy + Tislelizumab + Chemotherapy

    Patients receive 3 cycles of neoadjuvant therapy (every 21 days). Each cycle consists of: * Quad Shot radiotherapy (14-14.8 Gy/4 fractions on Day 1-2) * Tislelizumab (200 mg, Day 3) * Platinum-based doublet chemotherapy (Day 3): Carboplatin (AUC 5) or Cisplatin (75 mg/m²), combined with Pemetrexed (500 mg/m² for non-squamous) or Paclitaxel (175 mg/m² for squamous). Surgery is performed 4-6 weeks (±7 days) after the third cycle. Postoperative adjuvant therapy is administered at the investigator's discretion according to NCCN and CSCO guidelines.

    Radiation: Quad Shot Radiotherapy · Drug: Tislelizumab · Drug: Carboplatin or cisplatin · Drug: Pemetrexed or Paclitaxel

Interventions

  • RadiationQuad Shot Radiotherapy

    3.7 Gy per fraction, twice daily (with an interval of at least 6 hours), for 4 fractions over 2 consecutive days (Days 1-2 of each cycle). Total dose of 14.8 Gy per cycle, repeated every 3 weeks for 3 cycles (cumulative dose 44.4 Gy). Delivered using IMRT or VMAT.

  • DrugTislelizumab

    200 mg administered via intravenous infusion on Day 3 of each 21-day cycle for 3 cycles.

  • DrugCarboplatin or cisplatin

    Carboplatin (AUC 5) or Cisplatin (75 mg/m²) administered intravenously on Day 3 of each 21-day cycle for 3 cycles.

  • DrugPemetrexed or Paclitaxel

    Pemetrexed (500 mg/m²) for non-squamous NSCLC or Paclitaxel (175 mg/m²) for squamous NSCLC, administered intravenously on Day 3 of each 21-day cycle for 3 cycles.

05

What researchers measure

Primary outcomes

  1. Pathological Complete Response (pCR) Rate

    The proportion of patients with no residual viable tumor cells in the resected primary tumor and regional lymph nodes, as assessed by pathological review. Patients who do not undergo surgery will be considered as not achieving pCR.

    Time frame: After completion of neoadjuvant therapy and surgery (approximately 4-6 weeks after the last dose of neoadjuvant therapy)

Secondary outcomes

  1. Major Pathological Response (MPR) Rate

    The proportion of patients with ≤10% viable tumor cells in the resected specimen.

    Time frame: After completion of neoadjuvant therapy and surgery (approximately 4-6 weeks after the last dose of neoadjuvant therapy)

  2. R0 Resection Rate

    The proportion of patients achieving microscopically margin-negative resection.

    Time frame: At time of surgery

  3. Objective Response Rate (ORR) per RECIST v1.1

    The proportion of patients achieving a confirmed complete or partial response according to RECIST v1.1.

    Time frame: Up to 14 days before surgery

  4. Event-Free Survival (EFS) and 2-Year EFS Rate

    Time from enrollment to disease progression, local recurrence, distant metastasis, or death from any cause.

    Time frame: Up to 60 months after enrollment

  5. Overall Survival (OS) and 2-Year OS Rate

    Time from enrollment to death from any cause.

    Time frame: Up to 60 months after enrollment

  6. Incidence and Severity of Adverse Events (AEs), Serious Adverse Events (SAEs), and Immune-Related Adverse Events (irAEs)

    Evaluated according to NCI CTCAE v5.0.

    Time frame: From informed consent to 90 days after the last dose of study treatment

  7. Feasibility (Completion Rate of Neoadjuvant Therapy and Surgery)

    Proportion of patients completing 3 cycles of neoadjuvant therapy and undergoing surgery as planned; surgical delay rate.

    Time frame: Up to 4-6 weeks after the last dose of neoadjuvant therapy

  8. QS Radiotherapy-Related Toxicity

    Incidence and severity of radiation pneumonitis, esophagitis, dermatitis, myelosuppression, and myocarditis, graded according to NCI CTCAE v5.0.

    Time frame: Up to 90 days after the last dose of radiotherapy

06

Study locations

1 site
  • Wuhan Union Hospital
    Wuhan, Hubei 430022, China
07

Registry details

Key details

Study ID
NCT07845916
Lead sponsor
Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
Responsible party
Sponsor
First posted
Sep 29, 2026
Start date
Oct 10, 2026 (estimated)
Primary completion
Jun 30, 2027 (estimated)
Completion
Dec 31, 2031 (estimated)
Last update
Sep 29, 2026

Study contacts

Kunyu Yang, MD
Contact
yangkunyu@hust.edu.cn
0086-13995595360

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
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