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RecruitingNCT05550415Updated Sep 21, 2026

The Role of Simvastatin in The Epithelial-Mesenchymal Transition Process of Breast Cancer

A Phase 2 interventional study of Simvastatin 40mg and Placebo in Triple Negative Breast Cancer, Chemotherapy Effect and Simvastatin Adverse Reaction, sponsored by Universitas Indonesia. Recruiting at 1 site in Indonesia. Open to female participants aged 18 Years and older. Per ClinicalTrials.gov, last updated 2026-09-21.

Sponsored by Universitas Indonesia · Phase 2, Interventional, and Treatment

Phase
Phase 2
Study type
Interventional
Enrollment
26
Allocation
Randomized
Ages
18 Years and older
Sex
Female
01

Study summary

Introduction: Most cases of Triple Negative Breast Cancer (TNBC) have a high proliferation rate. TNBC is associated with a poor prognosis, a high recurrence rate, and a high incidence of distant metastases. The Epithelial-Mesenchymal Transition process (EMT) plays an essential role in the metastatic process. EMT markers were also more abundant in TNBC and contributed to a poorer TNBC prognosis. As an important EMT marker, the increased expression of vimentin also contributed to the increase in TNBC aggressiveness and resistance to chemotherapeutic agents. Through the mechanism of action in inhibiting the mevalonate pathway, statins can help inhibit the EMT process in metastases. Notably, simvastatin promotes the down-regulation of vimentin in breast cancer cells. The combination of statins and neoadjuvant chemotherapy (NAC) improves the cancer patient's response. This study is expected to evaluate the role of a combination between NAC and simvastatin on therapeutic response in TNBC patients through vimentin expression.

Methods: This study is a double-blind, randomized, placebo-controlled trial conducted in Dr. Cipto Mangunkusumo National Central General Hospital. An expected total of 26 TNBC patients will be assessed for eligibility and asked for informed consent. Patients with the plan to have ACT (Doxorubicin hydrochloride, Cyclophosphamide, Paclitaxel) chemotherapy regimen will receive either a combination of ACT-Simvastatin (40 mg/day) or ACT-Placebo. The biopsy will be taken pre-NAC to make the histopathological diagnosis and examine the expression of vimentin. Patients will be evaluated for adverse effects reaction every cycle and the clinical response after 8 cycles. The post-intervention biopsy will be conducted after the cycle finish. The pathological response and vimentin expression will be reviewed from the obtained samples.

02

Conditions studied

  • Triple Negative Breast Cancer
  • Chemotherapy Effect
  • Simvastatin Adverse Reaction

Keywords

  • Vimentin
  • Simvastatin
  • Epithelial-Mesenchymal Transition
  • Neoadjuvant Chemotherapy
  • Clinical Response
  • Pathological Response
03

Who can participate

Ages eligible
18 Years and older
Sexes eligible
Female
Accepts healthy volunteers
No

Inclusion criteria

  1. Female patients with advanced breast cancer (locally advanced and distantly advanced) with triple-negative molecular type confirmed by biopsy and immunohistochemical examination.
  2. The patient planned to receive 8 cycles of AC-T chemotherapy.
  3. Patient age > 18 years.
  4. Willing to participate in research by signing informed consent.

Exclusion criteria

Exclusion Criteria:

  1. The patient is pregnant or breastfeeding.
  2. Patients who have received chemotherapy or are on simvastatin therapy.
  3. Allergy to statins.
04

Study design

Phase
Phase 2
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Quadruple (Participant, Care provider, Investigator, Outcomes assessor)
Enrollment
26 participants (estimated)

Study arms

  • Experimental
    Simvastatin

    The group received standard treatment with simvastatin 40mg in capsule by oral route, once a day, for 21 days (every cycle of the chemotherapy regiment)

    Drug: Simvastatin 40mg

  • Placebo comparator
    Placebo

    The group received standard treatment with placebo 40mg in capsule by oral route, once a day, for 21 days (every cycle of the chemotherapy regiment)

    Drug: Placebo

Interventions

  • DrugSimvastatin 40mg

    The administration of Simvastatin 40 mg in addition to ACT regiment of neoadjuvant chemotherapy

    Also known as: Simvastatin

  • DrugPlacebo

    The administration of Placebo capsule 40 mg in addition to ACT regiment of neoadjuvant chemotherapy

    Also known as: Placebo oral capsule 40 mg

05

What researchers measure

Primary outcomes

  1. Vimentin Expression

    Vimentin expression is measured based on Histoscore (H-Score) with immunohistochemistry examination: * 0-50 : negative (0) * 51-100 : weak positive (1+) * 101-200 : moderate positive (2+) * 201-300 : strong positive (3+)

    Time frame: 6 months

Secondary outcomes

  1. Pathological Response

    Pathological Response as Measured by Miller-Payne system Evaluation before and after chemotherapy, divided into: 1. Grade 1: There is no significant change or reduction in cancer cells. 2. Grade 2: Reduction of \<30% cancer cells 3. Grade 3: Reduction of cancer cells between 30-90% 4. Grade 4: Reduction of \> 90% cancer cells 5. Grade 5 : There are no residual cancer cells. DCIS (Ductal Carcinoma In Situ) might be detected.

    Time frame: 6 months

  2. Clinical Response

    Clinical response based on WHO (World Health Organization) criteria: 1. Complete Response (CR): Disappearance 2. Partial Response (PR): 50% decrease 3. Stable Disease(SD): Neither PR nor PD criteria met 4. Progressive Disease (PD):25% increase; no CR, PR, or SD documented before increased disease

    Time frame: 6 months

06

Study locations

1 of 1 sites recruiting
  • Dr. Cipto Mangunkusumo National Central General Hospital
    Jakarta Pusat, DKI Jakarta 10430, Indonesia
    • Erwin D Yulian, MD · Contact · +6281315249627
    Recruiting
07

Registry details

Key details

Study ID
NCT05550415
Lead sponsor
Universitas Indonesia
Responsible party
Dr. dr. Erwin Danil Yulian, Sp.B (K) Onk (Head of Research Program, Division of Surgical Oncology, Universitas Indonesia) — Principal investigator
First posted
Sep 22, 2022
Start date
Aug 19, 2022
Primary completion
Nov 2026 (estimated)
Completion
Dec 2026 (estimated)
Last update
Sep 21, 2026

Study contacts

Erwin D Yulian, MD
Contact
erwin.yulian@ui.ac.id
+6281315249627
Erwin D Yulian, MD
principal investigator · Surgical Oncology Division, Department of Surgery, Universitas Indonesia
Tantri Hellyanti, MD
study director · Department of Pathological Anatomy, Universitas Indonesia
Shabrina Adzania, MD
study chair · Research Assistant, Department of Surgery, Universitas Indonesia

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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