An observational study in Root Resorption, sponsored by National University of Malaysia. Completed at 1 site in Malaysia. Open to participants aged 10 Years to 30 Years. Per ClinicalTrials.gov, last updated 2021-10-15.
Sponsored by National University of Malaysia · Observational
This study was conducted after obtaining approval from UKM Research Ethics Committee. Gingival Crevicular Fluid (GCF) from Orthodontic and non-orthodontic patients was collected with the consent of patients to detect Dentine sialophosphoprotein. Spectrometry analysis of Gingival Crevicular Fluid was conducted in collaboration with MIMOS Berhad.
Thirty patients were recruited having met the following inclusion criteria of (1) good general health, (2) good periodontal health, (3) caries-free, (4) good oral hygiene. The subjects were divided into 3 groups based on a radiographic assessment on the level of root resorption; normal (no root resorption), mild (less than 2 mm), and severe (more than 2 mm). The GCF samples were taken from the permanent central incisors of untreated subjects in the normal control group (n=14), while in the mild group, samples were collected from the permanent central incisors with mild root resorption (\<2 mm) in patients undergoing active orthodontic treatment after 6 months with radiographic evidence (mild group, n=5). Child patients with severe root resorption (>2 mm) were those with primary second molars undergoing physiological root resorption (severe group, n=11). The GCF samples were collected from the mesial and the distal gingival crevicular sulcus of the involved teeth using gingival fluid collection strips (Periopaper, Oraflow, Smithtown, N.Y.). The paper strips were placed within the sulcus (intrasulcular method) approximately 1 to 2 mm into the gingival sulcus and left in situ for 60 seconds. Every sample collection was taken 3 times at the same site with one-minute resting intervals to allow GCF to be replenished into the gingival sulcus.
Patients that come to the dental clinic for treatment
Exclusion Criteria:
Patients with non-orthodontic treatment and have no root resorption
Biological: DSPP level in GCF
Patients with orthodontic treatment and have mild to moderate root resorption
Biological: DSPP level in GCF
Child patients with non-orthodontic treatment and have severe root resorption
Biological: DSPP level in GCF
quantification of DSPP
Also known as: Root resorption
value of wavelength of DSPP in GCF from non-orthodontic patient with no root resorption
The absorbance values at a single wavelength at 450 nm
Time frame: 1 month
value of wavelength of DSPP in GCF from orthodontic patient with mild to moderate root resorption
The absorbance values at a single wavelength at 450 nm
Time frame: 1 month
value of wavelength of DSPP in GCF from child patient severe root resorption
The absorbance values at a single wavelength at 450 nm
Time frame: 1 month
Plan to share: Undecided
This study is completed, as verified in Oct 2021. You cannot join it, but the record below documents what was studied.
Get an email when the registry record changes — status, dates, results — or when someone posts here.
Sign in to followQuestions and observations about this study, from anyone following it. Not medical advice, and not a channel to the study team — their contact details are on the registry record.
Sign in to join the discussion. Reading takes no account; posting does. You choose a display name, and a pseudonym is the default.
Nothing here yet. If you are running this trial, taking part in it, or weighing whether to, this is the place to say so.
National University of Malaysia