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RecruitingNCT03993262Generate-BoostUpdated Sep 4, 2025

Trial to Evaluate Efficacy and Safety of Bortezomib in Patients With Severe Autoimmune Encephalitis

A Phase 2 interventional study of Bortezomib and Placebo in Autoimmune Encephalitis, sponsored by Jena University Hospital. Recruiting at 17 sites in Germany. Open to participants aged 18 Years and older. Per ClinicalTrials.gov, last updated 2025-09-04.

Sponsored by Jena University Hospital · Phase 2, Interventional, and Treatment

Phase
Phase 2
Study type
Interventional
Enrollment
50
Allocation
Randomized
Ages
18 Years and older
Sex
All
01

Study summary

Autoimmune Encephalitis is a disorder of the central nervous system caused by bodily substances, called antibodies. Antibodies normally help the body to prevent infections. However, in this disorder, the antibodies turn against the body itself and especially against cells in the brain and disturb the normal brain function. They are therefore called autoantibodies.

There is no specific therapy for patients with autoimmune encephalitis so far. At the moment, the symptoms are treated with approved medications such as cortisone and immunotherapies also used in oncology. These therapies are unspecified and aim to reduce the number of autoantibodies and to contain the autoimmune process. In this trial we aim to test a new therapy option: in this therapy the body cells producing autoantibodies will be specifically targeted by a substance called bortezomib.

The trial addresses patients with severe autoimmune encephalitis. The aim of the trial is to evaluate the efficacy and safety of bortezomib in patients with severe autoimmune encephalitis.

Read the detailed description

Autoimmune encephalitis is characterized by autoantibodies against neuronal surface antigens like the NMDA (N-methyl-D-aspartate) receptor or LGI1 (Leucin-rich glioma inactivated protein 1). So far, no specific therapy exists for this disease. Actual treatment includes combination therapies aiming for a reduction of pathogenic antibodies and containing the autoimmune process. In first line, patients are treated with plasmapheresis and cortisone. In second line, Rituximab and/or cyclophosphamide are administered. The response to these treatments are, however, often delayed and insufficient.

Therefore, we need a specific therapy aiming at the antibody-producing plasma cells.

Bortezomib is a proteasome inhibitor which interferes with NF-kB (nuclear factor kB) and the ubiquitin proteasome signaling pathway. Bortezomib acts preferably on cells with high protein synthesis - like plasma cells - and induces cell death in these cells. Bortezomib is used since more than a decade in chemotherapy of the multiple myeloma. Additionally, it is reported for systemic autoimmune diseases like lupus erythematodes that bortezomib leads to a depletion of plasma cells and therefore reduces the number of pathogenic antibodies and improves clinical outcome. The therapeutic potential of bortezomib for NMDAR encephalitis is described in a first case series with 5 patients.

02

Conditions studied

  • Autoimmune Encephalitis

Keywords

  • autoimmune disease
  • autoimmune encephalitis
  • bortezomib
  • NMDAR
  • LGI1
  • encephalitis
03

Who can participate

Ages eligible
18 Years and older
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  • Clinically diagnosed severe autoimmune encephalitis (defined as mRS ≥ 3) with autoantibodies to neuronal surface proteins in cerebrospinal fluid and / or serum
  • Pretreatment with rituximab
  • Age ≥18 years
  • signed informed consent
  • Women of childbearing potential (up to 2 years after menopause): negative pregnancy test

Exclusion criteria

Exclusion Criteria:

  • pregnancy/breast-feeding
  • acute infiltrative pulmonary and pericardial disease
  • malignant tumor under current chemotherapy
  • Simultaneous participation in another intervention study
  • Previous participation in this study
  • Known hypersensitivity to an ingredient of the investigational product
  • Continued therapy with glucocorticoids / rituximab during the study duration (last dose must be administered before the first dose of the investigational product)
04

Study design

Phase
Phase 2
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Quadruple (Participant, Care provider, Investigator, Outcomes assessor)
Enrollment
50 participants (estimated)

Study arms

  • Experimental
    Interventional

    1 to 3 cycles Bortezomib with 1,3mg/m2 body surface s.c. + 20mg dexamethasone p.o. on days 1, 4, 8 and 11 (= 1 cycle)

    Drug: Bortezomib

  • Placebo comparator
    Placebo

    1 to 3 cycles placebo (NaCl solution) s.c. + 20mg dexamethasone p.o. on days 1, 4, 8 and 11 (= 1 cycle)

    Drug: Placebo

Interventions

  • DrugBortezomib

    1 to 3 cycles Bortezomib with 1,3mg/m2 body surface s.c. + 20mg dexamethasone p.o. on days 1, 4, 8 and 11 (= 1 cycle)

  • DrugPlacebo

    1 to 3 cycles placebo (NaCl solution) s.c. + 20mg dexamethasone p.o. on days 1, 4, 8 and 11 (= 1 cycle)

    Also known as: isotonic NaCl solution

05

What researchers measure

Primary outcomes

  1. modified Rankin-Score (mRS)

    modified Rankin-Score from 0 = no symptoms to 6 = death

    Time frame: 17 weeks after first administration of the study drug

Secondary outcomes

  1. modified Rankin-Score (mRS)

    modified Rankin-Score from 0 = no symptoms to 6 = death

    Time frame: 3, 6, 9 and 13 weeks after first administration of the study drug; GCS Score also 17 weeks after first administration of the study drug

  2. Length of in-hospital stay / length of ICU stay

    Number of days in hospital or on ICU for each patient from first administration of the study drug until 17 weeks after first administration of the study drug

    Time frame: until 17 weeks after first administration of the study drug

  3. Immune response

    Antibody titer (in serum and liquor) and cellular immune response (FACS analysis of liquor)

    Time frame: at study start and 17 weeks after first administration of the study drug

  4. neurocognitive function assessed by Montreal Cognitive Assessment

    total score of the Montreal Cognitive Assessment (MoCA) (0 to max. 30 points = best possible result)

    Time frame: at study start and 17 weeks after first administration of the study medication

  5. neurocognitive function assessed by Mini-Mental Status Test

    total score of the Mini-Mental Status Test (MMST) (0 to max 30 points = best possible result)

    Time frame: at study start and 17 weeks after first administration of the study medication

  6. neurocognitive function assessed by Rey Auditory Verbal Learning Test

    total score of the Rey Auditory Verbal Learning Test (RAVLT) (memory performance assessed by 3 word lists which are read to the patient and should be recalled and repeated by the patient; different proceeding for the 3 word lists)

    Time frame: at study start and 17 weeks after first administration of the study medication

  7. neurocognitive function assessed by Neuropsychiatric Inventory Questionnaire

    total score of the Neuropsychiatric Inventory Questionnaire (NPI) (0 = best score to max 36 (patient) or 60 (caregiver)

    Time frame: at study start and 17 weeks after first administration of the study medication

  8. safety of Bortezomib regarding polyneuropathy, increase of liver enzymes and secondary infections

    number of polyneuropathy cases, number of increased liver enzymes, number of secondary infections

    Time frame: until 17 weeks after first administration of the study drug

  9. safety of Bortezomib regarding polyneuropathy

    number of polyneuropathy cases

    Time frame: until 17 weeks after first administration of the study drug

  10. safety of Bortezomib regarding increase of liver enzymes

    number of increased liver enzyme values

    Time frame: until 17 weeks after first administration of the study drug

  11. Secondary infections due to Bortezomib

    number of secondary infections

    Time frame: until 17 weeks after first administration of the study drug

  12. Hematotoxicity events due to Bortezomib

    number of hematotoxicity events

    Time frame: until 17 weeks after first administration of the study drug

  13. Gastrointestinal toxicity due to Bortezomib

    number of gastrointestinal toxicity events

    Time frame: until 17 weeks after first administration of the study drug

  14. total Glasgow Coma Scale (GCS)

    GCS from 3 to 15 points (sum of 3 subscores eye response (1 to 4 points), motor response (1 to 6 points), verbal response (1 to 5 points); highest score = best score; 1= worst score)

    Time frame: 3, 6, 9, 13 and 17 weeks after first administration of the study drug

  15. Destruction marker UCH-L1 (Ubiquitin carboxy-terminal hydrolase L1) in serum and liquor

    Analysis of destruction marker UCH-L1 in serum and liquor

    Time frame: at baseline visit and 17 weeks after first administration of the study drug

  16. Destruction marker Neurofilament light chain (in serum and liquor)

    Analysis of destruction marker Neurofilament light chain in serum and liquor

    Time frame: at baseline visit and 17 weeks after first administration of the study drug

  17. Destruction markers GFAP (glial fibrillary acidic protein) in serum and liquor

    Analysis of destruction marker GFAP in serum and liquor

    Time frame: at baseline visit and 17 weeks after first administration of the study drug

  18. Destruction marker TAU proteins in serum and liquor

    Analysis of destruction marker TAU in serum and liquor

    Time frame: at baseline visit and 17 weeks after first administration of the study drug

06

Study locations

17 of 17 sites recruiting
07

References and documents

Publications

  • Wickel J, Chung HY, Platzer S, Lehmann T, Pruss H, Leypoldt F, Gunther A, Scherag A, Geis C; GENERATE Study Group. Generate-Boost: study protocol for a prospective, multicenter, randomized controlled, double-blinded phase II trial to evaluate efficacy and safety of bortezomib in patients with severe autoimmune encephalitis. Trials. 2020 Jul 8;21(1):625. doi: 10.1186/s13063-020-04516-7. PubMed 32641101 ↗

Individual participant data

Plan to share: Undecided — It is not yet decided in which way and which data exactly will be shared with other researchers.

08

Registry details

Key details

Study ID
NCT03993262
Lead sponsor
Jena University Hospital
Collaborators
Federal Ministry of Education and Reserach (BMBF)
Responsible party
Christian Geis (Prof. Dr. med., Jena University Hospital) — Principal investigator
First posted
Jun 20, 2019
Start date
May 13, 2020
Primary completion
Sep 30, 2026 (estimated)
Completion
Dec 31, 2026 (estimated)
Last update
Sep 4, 2025

Study contacts

Christian Geis, Prof.
Contact
Christian.Geis@med.uni-jena.de
+49 (0) 3641 ext. 9323413
Jonathan Wickel, Dr.
Contact
Jonathan.Wickel@med.uni-jena.de
+49 (0) 3641 ext. 9323561
Christian Geis, Prof.
study director · University Hospital Jena

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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