An observational study in Periodontitis, sponsored by Gazi University. Completed. Open to participants aged 35 Years to 50 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2019-04-22.
Sponsored by Gazi University · Observational
The aims of our study were 1) to evaluate levels of gingival crevicular fluid( GCF) human cathelicidin peptide LL-37 and serum vitamin D3 in smoker and non-smoker patients with chronic periodontitis(CP) 2) to determine whether any correlation between GCF LL-37 and vitamin D3 serum levels exist and 3) to asses the correlation between clinical parameters and biochemical markers
Background: Cathelicidin LL-37, an antimicrobial peptide, is part of the host innate immune response in the oral cavity. The aims of this study are to evaluate; gingival crevicular fluid (GCF) levels of LL-37, serum vitamin D3 levels and periodontal clinical recordings in smoker and nonsmoker patients with chronic periodontitis (CP).
Methods:
This study consisted a total of 60 volunteers including 20 smoker patients with CP (CP-1 group), 20 non-smoker patients with CP (CP-2 group) and 20 periodontally healthy subjects (CTRL group). Prior to participation, the design and purpose of the research were explained to each subjects, and written informed consent form was obtained from 60 individuals before the study.
The periodontal status of the patients were determined by measuring the probing depth (PD), clinical attachment level (CAL), gingival index (GI), plaque index (PI) All clinical parameters were measured on six sites per tooth from the full-mouth teeth (mesiobuccal, distobuccal,midbuccal, mesiolingual, distolingual and midlingual) using a William's periodontal probe calibrated in millimeters by the same examiner. All samples were obtained on the day following clinical examinations of the individuals. The deepest six pockets site per individual were chosen for collection of GCF across both of the periodontitis groups. Six pocket locations showing a lack of clinical inflammation were also tested to guarantee collection of a sufficient volume of GCF across the CTRL groups.
GCF levels of LL-37 were measured by ELISA and serum levels of vitamin D3 were analysed by High-performance liquid chromatography (HPLC). Statistical analysis were performed.
1,635 studies on the registry are indexed under Periodontitis; 327 are open to participants now.
This study's enrollment of 60 is below the median of 90 across 428 observational studies indexed under Periodontitis.
Browse Periodontitis studies →Gazi University is the lead sponsor of 531 studies on the registry; 99 are open to participants now.
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The volunteers participitating in the study were choosen from among individuals scheduled to undergo either dental treatment or dental check at the Deparment of Perioodontology, Faculty of Dentistry, Gazi University, Ankara, Turkey.
Inclusion Criteria for chronic periodontitis groups:
Inclusion criteria for control group:
Smoking criteria for groups:
Exclusion Criteria:
Smoker patients with chronic periodontitis
Other: LL-37 levels in Gingival crevicular fluid · Other: Serum Vitamin D3 levels · Other: Clinical parameters
non-smoker patients with chronic periodontitis
Other: LL-37 levels in Gingival crevicular fluid · Other: Serum Vitamin D3 levels · Other: Clinical parameters
periodontally healthy patients
Other: LL-37 levels in Gingival crevicular fluid · Other: Serum Vitamin D3 levels · Other: Clinical parameters
Collected gingival crevicular fluid
Collected serum
Recorded plague index, gingival index, probing depth, clinical attachment level
Serum D3 Vitamin levels
The plasma levels of vitamin D3 were greater in the CP-2 group than in the CP-1 group. But, no statistically significant difference was determined between the CP groups (P\>0.05)
Time frame: Baseline
Gingival Crevicular fluid(GCF) LL-37 levels
The concentration of LL-37 in GCF was significantly higher in both of the CP groups than in the CTRL group (p\<0.001). Although GCF LL-37 concentration levels were greater in the CP-1 group than in the CP-2 group, the difference did not reach the statistical significance between the CP groups (p\>0.05).
Time frame: Baseline
Plaque index (PI)
The full-mouth and sample sites PI recordings were significantly higher in both CP groups (CP-1 and CP-2) than in the CTRL group (p\<0.001). were measured on six sites per tooth from the full-mouth teeth
Time frame: Baseline
GCF volume
The GCF volume was notably higher in both CP groups than in CTRL group (p\<0.001).
Time frame: Baseline
gingival index (GI)
The full-mouth and sample sites GI recordings were significantly higher in both CP groups (CP-1 and CP-2) than in the CTRL group (p\<0.001).
Time frame: Baseline
Probing depth (PD)
The full-mouth and sample sites PD recordings were significantly higher in both CP groups (CP-1 and CP-2) than in the CTRL group (p\<0.001).
Time frame: Baseline
Clinical attachment level (CAL)
The full-mouth and sample sites CAL recordings were significantly higher in both CP groups (CP-1 and CP-2) than in the CTRL group (p\<0.001).
Time frame: Baseline
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Gazi University