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Status unknownNCT03424772Updated Feb 8, 2018

Whole Genome Sequencing in the Detection of Rare Undiagnosed Genetic Diseases in Children in China

An observational study in Intellectual Disability, Multiple Congenital Anomaly and Rare Diseases, sponsored by Xinhua Hospital, Shanghai Jiao Tong University School of Medicine. Status unknown at 14 sites in China. Open to participants aged Up to 18 Years. Per ClinicalTrials.gov, last updated 2018-02-08.

Sponsored by Xinhua Hospital, Shanghai Jiao Tong University School of Medicine · Observational

The sponsor has not verified this record recently (last verified Feb 2018), so the status shown — last known as Recruiting — may be out of date.
Study type
Observational
Model
Family-based
Time perspective
Prospective
Enrollment
100
Ages
Up to 18 Years
Sex
All
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Study summary

To assess the indications and diagnostic efficiency of whole genome sequencing (WGS) in pediatric patients with unexplained intellectual disability/developmental delay, multiple congenital abnormalities and other rare and undiagnosed diseases

Read the detailed description

This project will recruit 100 rare, undiagnosed pediatric genetic disease families (core families: patients, patients' parents, immediate family members such as brothers and sisters, all of them can be enrolled whether they have disease or not, so generally 3, for a few cases 4 or 5) all over the country. The expert team will review the clinical materials, the molecular team will review the experimental process, and the bioinformatics team will review the chip, the analysis of whole exome sequencing data and screen the samples all over the country;

Whole-genome sequencing of 100 rare, undiagnosed pediatric genetic disease families (Illumina NovaSeq High-throughput Sequencer);

The study will provide preliminarily performance data on the comparison of whole exome data and whole genome data. In addition, it will generate the Chinese Consensus on Clinical Applications of Whole-genome sequencing in the Diagnosis of Birth Defects and Undiagnosed Rare Genetic Diseases in Children based on the statistical analysis of clinical phenotype and genotype association, which could guide the clinical application of pediatrics, laboratory testing and reporting.

Construction of the Chinese detection genome database of genetic disease

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Conditions studied

03

Who can participate

Ages eligible
Up to 18 Years
Sexes eligible
All
Accepts healthy volunteers
No
Sampling method
Non-probability sample

Study population

Undiagnosed Children with intellectual disability/developmental delay and/or multiple congenital abnormalites in China

Inclusion criteria

  1. Intelligence tests results of less than 40 (patients \<3 years old using the Gesell Developmental Scale for screening; patients of 3-6 years old using Little Wechsler Intelligence Scale for screening; patients >6 years old using Old Wechsler Intelligence Scale for screening).
  2. Neurodevelopmental defects can be expressed as mental retardation, motor development retardation, language delay, epilepsy, etc. May have or not have Multiple congenital abnormalities, families with more than one affected patient will be enrolled priority
  3. Families went through at least one of the high throughput technology(WES or CMA) and receive the negative result

Exclusion criteria

Exclusion Criteria:

  1. Intellectual disability caused by pregnancy, perinatal infection, ischemia, and hypoxia and other non-hereditary causes,
  2. Obvious genetic metabolic diseases (such as different types of genetic metabolic diseases, bone disease, fragile X syndrome, etc.);
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Study design

Observational model
Family-based
Time perspective
Prospective
Enrollment
100 participants (estimated)
Target follow-up
1 Year
Patient registry
Yes
Biospecimen retention
Samples with dna

Groups and cohorts

  • Patients with unexplained DD/ID

    Whole genome sequencing will be performed on pediatric patients with unexplained developmental delay(DD)/intellectual disability(ID), multiple congenital abnormalities and other rare and undiagnosed diseases.

    Diagnostic Test: Whole genome sequencing

Interventions

  • Diagnostic testWhole genome sequencing

    WGS will be performed for the trio

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What researchers measure

Primary outcomes

  1. Number of diagnosed families

    Families with rare and undiagnosed pediatric genetic disease will be benefitted by WGS.

    Time frame: 1 year

Secondary outcomes

  1. Numbers of pathogenic variants in different variation types

    WGS would have the potential to detect different types of genetic alterations, such as structure variations, point mutation, small insertion/deletion, trinucleotide repeat, etc. Some types could not be identified by exome sequencing and chromosomal microarray. The numbers of the pathogenic variants in these types will be calculated to examine the benefit of WGS.

    Time frame: 1 year

06

Study locations

14 of 14 sites recruiting
  • Peking Union Medical College Hospital
    Beijing, Beijing 100005, China
    Recruiting
  • Children's Hospital, Capital Institute of Pediatrics
    Beijing, Beijing 100020, China
    • Xiaoli Chen, PhD · Contact · cxlwx@sina.com
    • Xiaoli Chen, PhD · Sub investigator
    Recruiting
  • Department of Pediatrics, Peking University First Hospital
    Beijing, Beijing 100034, China
    • Yuwu Jiang, PhD · Contact · jiangyw@263.net
    • Yuwu Jiang, PhD · Sub investigator
    Recruiting
  • The Maternal & Child Health Hospital, The Children's Hospital, The Obstetrics & Gynecology Hospital of Guangxi Zhuang Autonomous Region
    Nanning, Guangxi 530005, China
    Recruiting
  • The Maternal and Child Health Hospital of Hunan Province
    Changsha, Hunan 410008, China
    Recruiting
  • Xiangya Hospital, Central-south University / Hunan Jiahui genetics hospital
    Changsha, Hunan 410008, China
    Recruiting
  • Hunan Children's Hospital
    Changsha, Hunan 410011, China
    Recruiting
  • Nanjing maternal and children hospital
    Nanjing, Jiangsu 210004, China
    • Zhengfeng Xu, PhD · Contact · njxzf@126.com
    • Zhengfeng Xu, PhD · Sub investigator
    Recruiting
  • Ruijin Hospital affiliated to Shanghai Jiaotong University
    Shanghai, Shanghai 200025, China
    Recruiting
  • Children's Hospital of Shanghai
    Shanghai, Shanghai 200041, China
    Recruiting
  • Shanghai Institute for Pediatric Research
    Shanghai, Shanghai 200092, China
    • Xiaomei Luo, MD, PhD · Contact · yuyongguo@shsmu.edu.cn · +86-21-25076466
    • Yongguo Yu, MD, PhD · Principal investigator
    Recruiting
  • Xin Hua Hospital, Shanghai Jiaotong University School of Medicine
    Shanghai, Shanghai 200092, China
    Recruiting
  • Shanghai Children's Medical Center
    Shanghai, Shanghai 201712, China
    Recruiting
  • Wenzhou Central Hospital
    Wenzhou, Zhejiang 325099, China
    • Shaohua Tang, PhD · Contact · tsh006@163.com
    • Shaohua Tang, PhD · Sub investigator
    Recruiting
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References and documents

Publications

  • Park ST, Kim J. Trends in Next-Generation Sequencing and a New Era for Whole Genome Sequencing. Int Neurourol J. 2016 Nov;20(Suppl 2):S76-83. doi: 10.5213/inj.1632742.371. Epub 2016 Nov 22. PubMed 27915479 ↗

Individual participant data

Plan to share: Undecided — We have not yet decided which part of individual participant data(IPD) can be shared.

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Registry details

Key details

Study ID
NCT03424772
Lead sponsor
Xinhua Hospital, Shanghai Jiao Tong University School of Medicine
Responsible party
Yongguo Yu (Associate chief physician, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine) — Principal investigator
First posted
Feb 7, 2018
Start date
Jan 18, 2018
Primary completion
Dec 2018 (estimated)
Completion
Mar 2019 (estimated)
Last update
Feb 8, 2018

Study contacts

Yu Sun, PhD
Contact
sunyu@xinhuamed.com.cn
+86-25-25076466
Xiaomei Luo, Ms
Contact
luoxiaomei@xinhuamed.com.cn
+86-25-25076466
Yongguo Yu, MD, PhD
principal investigator · Specify Unaffiliated

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

Not currently enrolling

This study is status unknown, as verified in Feb 2018. You cannot join it, but the record below documents what was studied.

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