An observational study in Crohn's Disease and Ulcerative Colitis, sponsored by University Hospital, Clermont-Ferrand. Completed at 1 site in France. Open to participants aged 18 Years and older, including healthy volunteers. Per ClinicalTrials.gov, last updated 2019-02-04.
Sponsored by University Hospital, Clermont-Ferrand · Observational
The M2iSH laboratory showed with two previous clinical trials that Crohn's Disease (CD) macrophages present i) a defect to control Adherent-Invasive Escherichia coli (AIEC) infection related to polymorphisms associated with CD; ii) a CD - specific cytokine secretion profile after an AIEC infection and intestinal inflammation dependent; iii) a modification of the response of CD macrophages at a basal state and after the AIEC infection. These results consolidate the hypothesis of a defect specific to CD macrophages.
That's why, the primary purpose of this study is to realize a proteomic analysis of macrophages of CD patients infected or not with AIEC and to compare them to Ulcerative Colitis (UC) patients and healthy volunteers.
The macrophages characterization will be realized at a basal state and in response or not to AIEC.
Investigator aimed to 1) better understand the differences between macrophages of CD patients, UC patients and healthy volunteers but also the impact of AIEC infection on these macrophages to provide key informations to detect and characterize the defect of these macrophages; 2) highlight one or several protein in CD macrophages that could represent, in a long term, a potential therapeutic target of CD.
Patients with Crohn's disease
Exclusion Criteria:
44 patients
Other: Proteomic analysis
22 patients
Other: Proteomic analysis
22 patients
Other: Proteomic analysis
Proteomic analysis of Crohn's disease macrophages in response or not to Adherent-Invasive Escherichia coli
Compare the proteomic profile of macrophages of patients with CD to patients with UC and healthy subjects in the basal state
Compare concentrations of different protein types in the supernatant of macrophages from patients with CD through proteomic approaches, compared with patients with UC or controls.
Time frame: at day 1
Comparison of concentrations of different protein types in the supernatant of macrophages
Time frame: at day 1
presence of subgroups of patients with CD based on the proteomic profile of their macrophages.
Time frame: at day 1
association between these subgroups of patients with CD and biological parameters.
Time frame: at day 1
associations between the proteomic profiles of macrophages of patients with CD, UC or control subjects and fecal AIEC status
Time frame: at day 1
associations between the levels of entry, survival and multiplication of AIEC bacteria
Time frame: at day 1
impact of anti-TNF treatment on the proteomic profile (s) observed for macrophages of patients with CD
Time frame: at day 1
associations between the Proteomic profile (s) of the macrophages derived from monocytes and their AIEC status
Time frame: at day 1
associations between the Proteomic profile (s) of tissue macrophages and their AIEC status
Time frame: at day 1
This study is completed, as verified in Feb 2019. You cannot join it, but the record below documents what was studied.
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University Hospital, Clermont-Ferrand