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WithdrawnNCT03275792Updated Nov 9, 2020

Shiga Toxin Producing Escherichia Coli (STEC) Volume Expansion

A Phase 1 interventional study of D5-0.9%NS and Routine home oral rehydration in Hemolytic-Uremic Syndrome, sponsored by University of Calgary. Withdrawn at 1 site in Canada. Open to participants aged 6 Months to 18 Years. Per ClinicalTrials.gov, last updated 2020-11-09.

Sponsored by University of Calgary · Phase 1, Interventional, and Treatment

Why this study was withdrawn
Funding not obtained.
Phase
Phase 1
Study type
Interventional
Enrollment
0
Allocation
Randomized
Ages
6 Months to 18 Years
Sex
All
01

Study summary

This study will provide feasibility data regarding the conduct of a clinical trail evaluating the use of early aggressive inpatient intravenous rehydration in children with Shiga Toxin producing E. coli infection.

Read the detailed description

Background: Shiga toxin-producing Escherichia coli (STEC) cause a spectrum of disease, ranging from asymptomatic carriage to bloody diarrhea and the hemolytic uremic syndrome (HUS). HUS is caused by a toxin that destroys red blood cells, consumes platelets and impairs kidney function. HUS results in morbidity and even death in otherwise healthy children. Over the last 30 years however, there has been extremely limited progress in preventing acute and long-term complications in children with STEC infection. However, it is believed that Shiga toxins generate clots or blockages in the kidneys that damage it much the way strokes cause brain damage. There is emerging evidence that if children with STEC infection are recognized early, then the interval between diarrhea onset and the presence of HUS could be exploited to preserve kidney function through the use of intravenous rehydration.

Study Design: The investigators propose to conduct the first randomized clinical trial of volume expansion therapy in children with STEC infection. Employing Alberta's unique province-wide microbiology network and its only two pediatric tertiary care centres, the investigators will conduct a proof of principal feasibility study that evaluates novel technologies to identify STEC infected children and those at risk for HUS.

Objectives: The primary outcome will be process: number of children recruited. Secondary outcomes will include: 1) resources: retention; refusal; compliance; eligibility criteria; questionnaires; data collection tools; and time requirements; 2) management: capacity and impact on clinical services; 3) scientific: utility of point-of-care STEC diagnostics; use of urine biomarkers to identify high risk children, monitoring of kidney injury and response to therapy; and safety.

Significance: This pilot will provide the necessary data to integrate novel technologies into the design and conduct of a multicentre, multinational, clinical trial that will reduce morbidity and mortality from STEC infection.

02

Conditions studied

  • Hemolytic-Uremic Syndrome

Keywords

  • Shiga-Toxigenic Escherichia coli
  • Child
03

Who can participate

Ages eligible
6 Months to 18 Years
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  1. Age \<18.0 years;
  2. STEC infection [positive culture OR antigen OR polymerase chain reaction test for Stx/gene];
  3. Day of illness 1-10: Children who develop HUS will do so by day #14 of illness;8 restricting enrolment to the first 10 days will ensure all participants are at risk of HUS.

Exclusion criteria

Exclusion Criteria:

  1. Evidence of evolving HUS: A) Hematocrit \<30% OR B) Platelet count \<150 x 109/L;
  2. Responsible physician desires patient admission (therefore unable to randomize);
  3. Unable to contact family within 48 hours of positive stool test;
  4. Patient with history of atypical HUS;
  5. Chronic disease limiting fluid volumes administered (e.g. impaired cardiac function)
04

Study design

Phase
Phase 1
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Single (Outcomes assessor)
Enrollment
0 participants (actual)

Study arms

  • Experimental
    Admission/Intravascular Volume Expansion

    1. Infusion of 40 mL/kg of 0.9% normal saline (NS) IV over 60 minutes 2. 0.9% NS with 5% dextrose at 150% of standard maintenance volume 3. If urine output is \<0.5 ml/kg/hr over a 12-hour period (AKI Stage 2), repeat 20 mL/kg bolus or boluses of 0.9% NS will be infused as long as there are no signs of central volume overload 4. Oral fluids ad lib along with strict input/output documentation 5. Fluids will be restricted if: A) Anuria for 12 hours OR B) Evidence of fluid overload 6. Daily laboratory tests and in-person assessment until inpatient discharge criteria reached: A) 2 - 4 days since symptom onset AND rising platelet count (\>5% increase) documented over 48 hours in a clinically well child B) ≥5 days since symptom onset AND stable platelet count (\<5% decrease) documented over 48 hours in a clinically well child 7. Repeat hematocrit, platelet, renal function 24 and 72-hours post-discharge.

    Drug: D5-0.9%NS

  • Active comparator
    Outpatient Observation

    1. Following standard emergency department (ED) care \[volume status assessed; dehydration corrected employing oral rehydration in children with mild to moderate dehydration (most common); IV if severe (rarely)\], children are discharged with saline lock IV (routine procedure across Canadian pediatric EDs). 2. Oral fluids (preferably electrolyte maintenance solutions) ad lib following ED discharge 3. Additional health assessments as required 4. Daily blood tests at a local laboratory with results conveyed daily to the site-investigator until outpatient discharge criteria achieved; no in-person assessment given logistics (i.e. distance), impact on family, and mirroring of standard practice A) 2 - 4 days since symptom onset AND rising platelet count (\>5% increase) documented over 48 hours in a clinically well child B) ≥5 days since symptom onset AND stable platelet count (\<5% decrease) documented over 48 hours in a clinically well child

    Drug: Routine home oral rehydration

Interventions

  • DrugD5-0.9%NS

    Admission for intravascular volume expansion

  • DrugRoutine home oral rehydration

    Routine oral fluids as is given at home to all children with acute diarrheal disease

05

What researchers measure

Primary outcomes

  1. Number of children enrolled in the study protocol

    The number of children recruited per month per site will be calculated and will be related to the number screened, number eligible, and number consented.

    Time frame: at the end of the 24 month study recruiting period

Secondary outcomes

  1. The proportion of children enrolled in each study arm who develop adverse events

    For participants enrolled in each study arm we will quantify the proportion that are admitted to Intensive Care Units, the proportion requiring respiratory support (CPAP, BiPAP, endotracheal intubation), hypoxia defined by the administration of supplemental oxygen, and evidence of congestive heart failure defined by blinded independent reviewers.

    Time frame: at the end of the 24 month study recruiting period

  2. Retention

    The proportion of children who complete the study protocol

    Time frame: at the end of the 24 month study recruiting period

  3. Time requirements

    We will quantify the number of hours children remain admitted and to which clinical units

    Time frame: at the end of the 24 month study recruiting period

  4. Child/family perspectives

    7-item likert scales will be employed to evaluate perspectives of parents and participants as appropriate related to study protocols, procedures and participation

    Time frame: at the end of the 24 month study recruiting period

  5. compliance/adherence

    The proportion of children enrolled in each study arm who comply with the key interventions of the respective study arms

    Time frame: at the end of the 24 month study recruiting period

  6. data collection tool performance

    Individual data fields will be audited with respect to data quality, reliability, completeness, timeliness of completion

    Time frame: at the end of the 24 month study recruiting period

  7. Impact on clinical services

    We will qualitatively explore with the department leads at the respective institutions if the study protocol had any impact on clinical care provided either to the admitted patients or to other patients on their services

    Time frame: at the end of the 24 month study recruiting period

  8. Cost

    We will quantify the costs per child in each study arm

    Time frame: at the end of the 24 month study recruiting period

Other outcomes

  1. Point-of-Care STEC diagnosis

    diagnostic accuracy compared with standard culture

    Time frame: at the end of the 24 month study recruiting period

  2. Urine biomarkers

    ability to predict progression to AKI and HUS

    Time frame: at the end of the 24 month study recruiting period

  3. Point-of-Care STEC diagnosis

    turnaround time

    Time frame: at the end of the 24 month study recruiting period

  4. Point-of-Care STEC diagnosis

    proportion O157 vs other STEC

    Time frame: at the end of the 24 month study recruiting period

06

Study locations

1 site
  • Alberta Children's Hospital
    Calgary, Alberta T3B 6A8, Canada
07

References and documents

Publications

  • Imdad A, Mackoff SP, Urciuoli DM, Syed T, Tanner-Smith EE, Huang D, Gomez-Duarte OG. Interventions for preventing diarrhoea-associated haemolytic uraemic syndrome. Cochrane Database Syst Rev. 2021 Jul 5;7(7):CD012997. doi: 10.1002/14651858.CD012997.pub2. PubMed 34219224 ↗

Individual participant data

Plan to share: Undecided

08

Registry details

Key details

Study ID
NCT03275792
Lead sponsor
University of Calgary
Responsible party
Sponsor
First posted
Sep 8, 2017
Start date
May 2020 (estimated)
Primary completion
Apr 2021 (estimated)
Completion
Apr 2021 (estimated)
Last update
Nov 9, 2020

Study contacts

Stephen Freedman, MDCM, MSc
principal investigator · University of Calgary

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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