An interventional study of other : PRF in Periodontal Disease, Periodontal Bone Loss and Osteoclastic Bone Loss, sponsored by Meenakshi Ammal Dental College and Hospital. Completed. Open to participants aged 25 Years to 35 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2017-07-26.
Sponsored by Meenakshi Ammal Dental College and Hospital · Not applicable, Interventional, and Other
The present study is an effort to investigate the hypothesis that Platelet Rich Fibrin/Biphasic Calcium Phosphate may play a role in inhibiting osteoclasts differentiation and in bone loss.
Study design
Fifty-five individuals from a suburban population were recruited. Out of them, 5 individuals refused to participate in the study. Finally, 50 participants were selected and divided into two groups, control group [Healthy individuals]: 25 Males/Females, aged 25 to 35 years and test group [Chronic periodontitis patients]: 25 Males/Females aged 30 to 45 years.
Demographic variables and periodontal parameters were measured in both the groups.The investigators explored the effect of Platelet rich fibrin (PRF)/Biphasic calcium phosphate (BCP) on differentiation and survival of osteoclasts obtained from peripheral blood of Chronic Periodontitis patients.
297 studies on the registry are indexed under Alveolar Bone Loss; 76 are open to participants now.
This study's enrollment of 50 is above the median of 25 across 254 interventional studies indexed under Alveolar Bone Loss.
Browse Alveolar Bone Loss studies →Meenakshi Ammal Dental College and Hospital is the lead sponsor of 20 studies on the registry; 2 are open to participants now.
Counted across the registry records on this site, refreshed daily.
Inclusion criteria: (Control group)
Inclusion Criteria: (Test group)
Exclusion Criteria: ( both the groups)
Mononuclear cells isolated from the peripheral blood of 25 chronic periodontitis patients were interveened with 1ml/well preparation of Platelet rich fibrin(PRF), Biphasic calcium phosphate (BCP) and PRF and BCP combination in-vitro.
Other: other : PRF
Mononuclear cells isolated from the peripheral blood of healthy individuals in-vitro.
Other: other : PRF
mononuclear cells from the peripheral blood of chronic periodontitis patients were intervened with PRF, BCP and PRF/BCP combination
Also known as: others : BCP, others : PRF/BCP combination
probing pocket depth
probing pocket dept was measured using Williams periodontal probe at base line
Time frame: base line
Protein expression on osteoclast cells was estimated with BCP, PRF alone and BCP/PRF combination.
Protein expression on cells was analyzed using Western blot in all the groups
Time frame: 24 hours from baseline
The activity of caspase-3 in OCs.
The activity of caspase-3 was seen in OCs in all the groups
Time frame: 24 hours from base line
No study locations are listed for this record.
Plan to share: No
This study is completed, as verified in Jul 2017. You cannot join it, but the record below documents what was studied.
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Meenakshi Ammal Dental College and Hospital