CClinicalTrials.gg
Enrolling by invitationNCT02422875Updated Sep 30, 2025

Comparative Autoantibody and Immunologic Cell Marker Study

An observational study in Autoimmune Diseases, Lupus Erythematosus, Systemic and Communicable Diseases, sponsored by Emory University. Enrolling by invitation at 3 sites in United States. Open to participants aged 18 Years and older, including healthy volunteers. Per ClinicalTrials.gov, last updated 2025-09-30.

Sponsored by Emory University · Observational

Study type
Observational
Model
Case-control
Time perspective
Cross-sectional
Enrollment
2,500
Ages
18 Years and older
Sex
All
01

Study summary

The purpose of this study is to compare immune phenotype, function, and specificity of B lymphocytes from different developmental stages in autoimmune patients to B cells from infectious disease patients and healthy controls.

Read the detailed description

Systemic lupus erythematosus (SLE) is an autoimmune disease (in autoimmune illness, the immune system in the body attacks it's own cells, leading to illness). It is not completely understood how this disease develops in the body. In a normal person, there is a tolerance of antigens (substances that make antibodies, which protect the body from disease-causing agents). Research in mice suggests that defects in certain types of cells can make the body lose this tolerance, therefore recognizing antigens made in the body as foreign, and mounting an immune response to the "self", thus causing autoimmune disease. In this study, the researchers will look at these potentially defective cells in people with SLE and other autoimmune diseases and compare them to cells in healthy participants, as well as looking at the blood of first-degree relatives of people with autoimmune disease.

The study involves blood draws and bone marrow aspirates. Participants may be asked to donate 2/3 to about 9 tablespoons of blood. The volume of blood needed will depend on the experiment being done as different numbers of cells are necessary to run different experiments. Study participants may return for additional blood draws will not donate blood more than twice a week, and will not have more than 16 tablespoons of blood drawn in a one-month period. Participants donating bone marrow will have about 3 ½ tablespoons of bone marrow obtained, which will be drawn with a large needle from the bone located in the back of the hip. Bone marrow participants may be asked to donate up to 7 tablespoons of blood as well, in order to correlate the blood with the bone marrow sample and the populations of cells residing in each. Participants donating bone marrow may donate more than once, but must wait a minimum of 8 weeks between donations.

02

Conditions studied

  • Autoimmune Diseases
  • Lupus Erythematosus, Systemic
  • Communicable Diseases

Keywords

  • Sjögren's Syndrome
  • Scleroderma
  • Myositis
  • Juvenile Idiopathic Arthritis
  • Rheumatoid Arthritis
  • Inflammatory Arthritis
  • Undifferentiated Connective Tissue Disease
  • Idiopathic thrombocytopenic purpura
  • Graft vs Host Disease
  • Autoimmune Lymphoproliferative Syndrome
  • Immunoglobulin G4 (IgG4)-related disease
  • Hepatitis C
  • Epstein Barr Virus (infectious mononucleosis - EBV)
  • Sepsis
  • Guillain-Barre syndrome
  • Mycoplasma pneumoniae
  • Human Immunodeficiency Virus
03

Who can participate

Ages eligible
18 Years and older
Sexes eligible
All
Accepts healthy volunteers
Yes
Sampling method
Non-probability sample

Study population

  • Subjects with certain autoimmune conditions or if subjects have brothers, sisters, mother, father, or children with an autoimmune condition
  • Subjects with certain infectious diseases
  • Subjects who are without an autoimmune condition or infectious disease
  • Subjects who have received or will receive a vaccination as part of their regular standard of care from one of their healthcare providers

Inclusion criteria

  • Signed written informed consent by the subject or, if the subject is unable to provide informed consent, the subject's legal representative may provide consent.
  • Subjects can be of either gender
  • Subjects with autoimmune diseases, and Systemic Lupus Erythematosus (SLE) patients will fulfill the American College of Rheumatology Classification criteria for SLE to be determined by their treating physician but may have incomplete criteria (\<4 items). SLE patients are not restricted by treatment or by disease activity as determined by Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) or Systemic Lupus Activity Measure (SLAM) score

    1. Subjects with acute exacerbations of their disease, including hospitalized patients
    2. First-degree relatives of subjects with active disease
  • Subjects who have received or will receive a vaccination may be enrolled for bone marrow aspirates before and/or after vaccination. Vaccination will have been done by the subject's healthcare provider or through another outside source.
  • Subjects may have a screening blood draw performed in cases where a certain subset of cells or antibody titer is desired. This may be followed by additional blood draws and/or bone marrow aspiration after the ideal candidates have been identified.
  • Subjects who have been diagnosed with HIV or another infectious disease
  • Subjects taking biologic and/or immune modulatory agents in diseases such as cancer, allergy, and pulmonary diseases will be enrolled.
  • Healthy controls must be free of acute or chronic disease at the time of bone marrow donation. Healthy controls that are first-degree relatives of subjects with active disease will be enrolled as well.

Exclusion criteria

Exclusion Criteria:

  • Poor venous access
  • Subjects who have had side effects to local anesthetics such as lidocaine and who are on blood thinners such as warfarin
  • Normal controls must be free of acute or chronic diseases or medications that may affect the assay (as determined by the investigator).
  • For subjects donating bone marrow, insufficient access to the iliac crest such that the periosteum and bone is hindered based on normal aspiration procedures.
  • Pregnant or lactating women may not donate bone marrow.
04

Study design

Observational model
Case-control
Time perspective
Cross-sectional
Enrollment
2,500 participants (estimated)
Target follow-up
3 Days
Patient registry
Yes
Biospecimen retention
Samples with dna

Groups and cohorts

  • Healthy Controls

    Subjects are healthy persons without any autoimmune conditions or infectious diseases

  • Autoimmune Disease

    Subjects diagnosed with autoimmune disease including but not limited to: Systemic Lupus Erythematosus (SLE), Sjögren's Syndrome (SS), Scleroderma, Myositis, Juvenile Idiopathic Arthritis (JIA), Rheumatoid Arthritis (RA), inflammatory arthritis, undifferentiated connective tissue disease, idiopathic thrombocytopenic purpura (ITP), Graft vs Host Disease (GVHD), Autoimmune Lymphoproliferative Syndrome (ALPS) and IgG4-related disease

  • Infectious Disease

    Subjects diagnosed with an infectious disease including but not limited to: Hepatitis C, Epstein Barr Virus (infectious mononucleosis - EBV), Sepsis, Guillain-Barre syndrome (GBS), Mycoplasma pneumoniae or Human Immunodeficiency Virus (HIV)

  • Autoimmune - Family

    Subjects have a brother, sister, mother, father, or child with an autoimmune disease

  • Vaccination

    Subjects have received or will receive a vaccination as part of regular standard of care from their healthcare provider or other outside source

05

What researchers measure

Primary outcomes

  1. Distribution of autoreactive B cells within bone marrow

    B cells will be analyzed using flow cytometry.

    Time frame: Baseline

06

Study locations

3 sites
  • Emory University Hospital
    Atlanta, Georgia 30322, United States
  • Emory University Winship Cancer Institute
    Atlanta, Georgia 30322, United States
  • Grady Health System
    Atlanta, Georgia 30322, United States
07

Registry details

Key details

Study ID
NCT02422875
Lead sponsor
Emory University
Collaborators
National Institute of Allergy and Infectious Diseases (NIAID)
Responsible party
Ignacio Sanz (Professor, Emory University) — Principal investigator
First posted
Apr 21, 2015
Start date
Aug 2012
Primary completion
May 2028 (estimated)
Completion
May 2028 (estimated)
Last update
Sep 30, 2025

Study contacts

Ignatio Sanz, MD
principal investigator · Emory University

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

Interested in this study?

Eligibility is decided by the study team. Share this record with your doctor or contact the team directly.

No contact was published for this record. The registry link below has the sponsor’s details.

Follow this study

Get an email when the registry record changes — status, dates, results — or when someone posts here.

Sign in to follow

Discussion

Questions and observations about this study, from anyone following it. Not medical advice, and not a channel to the study team — their contact details are on the registry record.

Sign in to join the discussion. Reading takes no account; posting does. You choose a display name, and a pseudonym is the default.

Nothing here yet. If you are running this trial, taking part in it, or weighing whether to, this is the place to say so.

Start the discussion