An interventional study of spirulina gel exracted by 1.5% calcium chloride (CaCl2) aqueous solution. and scaling and root planing in Periodontitis and Periodontitis Stage II, sponsored by Misr University for Science and Technology. Not yet recruiting. Open to participants aged 25 Years to 40 Years. Per ClinicalTrials.gov, last updated 2026-10-26.
Sponsored by Misr University for Science and Technology · Not applicable, Interventional, and Treatment
This randomized clinical trial aims to evaluate the clinical and immunological effects of locally delivered Spirulina gel as an adjunct to scaling and root planing (SRP) in patients with Stage II, Grade B periodontitis. The study will include 20 male and female participants aged 25-40 years who will be randomly allocated into two groups.
The main questions are whether the addition of Spirulina gel to SRP improves periodontal clinical parameters, particularly probing pocket depth (PPD) and clinical attachment level (CAL), and whether it reduces the levels of interleukin-1β (IL-1β) and tumor necrosis factor-alpha (TNF-α) in gingival crevicular fluid (GCF).
Participants in Group A will receive SRP alone, while participants in Group B will receive SRP followed by local application of Spirulina gel into selected periodontal pockets. All participants will undergo periodontal assessments at baseline, 15 days, and 6 months after treatment. Clinical evaluations will include plaque index, gingival index, PPD, and CAL. GCF samples will be collected at the same time points and analyzed using enzyme-linked immunosorbent assay (ELISA) to measure IL-1β and TNF-α levels.
The findings will help determine whether adjunctive local application of Spirulina gel provides additional clinical and immunological benefits compared with SRP alone in the management of Stage II, Grade B periodontitis.
1,664 studies on the registry are indexed under Periodontitis; 328 are open to participants now.
This study's planned enrollment of 20 is below the median of 45 across 1,206 interventional studies indexed under Periodontitis.
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Exclusion Criteria:
Non-surgical periodontal therapy approach including scaling and root planning.
Other: scaling and root planing
Non-surgical periodontal therapy approach, including scaling and root planning, with injection of Spirulina gel into pockets with depth ≤5 mm and CAL 3-4 mm.
Other: spirulina gel exracted by 1.5% calcium chloride (CaCl2) aqueous solution.
The constituents of the spirulina-containing gel were prepared in the following proportion: Spirulina powder (4gm), Gelatin (12 gm), Glycerin (wetting agent) 0.2ml, Peppermint oil (flavoring agent)-0.1 ml, Sodium saccharin (sweetening agent) 0.1 mL, and Purified water q.s. 100ml. All the ingredients were homogenized using a homogenizer to obtain the gel. All the ingredients of the gel served as an inert vehicle for the drug
non surgical periodontal therapy
clinical attachment level
Assessment of Clinical attachment level from Cemento-enamel junction to the base of periodontal pocket
Time frame: baseline and 6 months
Probing depth
Assessment of probing depth from marginal gingiva to the base of periodontal pocket
Time frame: baseline and 6 months
Plaque index
assessment amount of plaque on tooth surface
Time frame: baseline, 15 days, and 6 months)
Gingival index
assessment of gingival inflammation
Time frame: baseline, 15 days, and 6 months
Assessment of tumor necrosis factor alpha (TNF-alpha) and interleukin-1 (IL-1) from
Assessment of tumor necrosis factor alpha (TNF-alpha) and interleukin-1 (IL-1) from gingival crevicular fluid by using filter paper - assessment by ELISA
Time frame: baseline, 15 days, and 6 months
No study locations are listed for this record.
Plan to share: Undecided
No publications or documents are linked to this record.
From the registry record's own update history. This site started tracking changes on Sep 25, 2026; for anything earlier, see the record history on ClinicalTrials.gov ↗
This study is not yet recruiting, as verified in Oct 2026. You cannot join it, but the record below documents what was studied.
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Misr University for Science and Technology