An interventional study of Cyperus articulatus in Chronic Apical Periodontitis, sponsored by Minia University. Recruiting at 1 site in Egypt. Open to participants aged 22 Years to 45 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-10-23.
Sponsored by Minia University · Not applicable, Interventional, and Treatment
Successful endodontic treatment depends on effective elimination of microorganisms from the infected root canal system and prevention of reinfection. However, complete disinfection remains difficult because of the complex anatomy of the root canal system, allowing residual microorganisms to persist and contribute to persistent apical periodontitis and treatment failure.
Mechanical instrumentation facilitates root canal shaping and irrigant penetration but cannot contact all canal walls. Consequently, microorganisms may persist within biofilms and dentinal tubules, making complete disinfection difficult. Therefore, intracanal medicaments are used as an adjunct to chemomechanical preparation, particularly in necrotic teeth with apical periodontitis.
Calcium hydroxide is the gold-standard intracanal medicament because of its antibacterial activity, ability to neutralize bacterial endotoxins, and capacity to promote mineralized tissue formation and periapical healing. Its antimicrobial effect is attributed to the release of hydroxyl ions that create a highly alkaline environment.
Despite these advantages, calcium hydroxide has limited efficacy against resistant microorganisms, particularly Enterococcus faecalis, and its activity may be reduced by the buffering effect of dentin. Consequently, researchers have investigated novel vehicles and bioactive compounds to enhance its antimicrobial performance.
The vehicle mixed with calcium hydroxide influences hydroxyl ion release, diffusion, and antimicrobial activity. Although sterile saline is the conventional aqueous vehicle, increasing interest has focused on natural products with antimicrobial, anti-inflammatory, antioxidant, and analgesic properties.
Cyperus articulatus essential oil has demonstrated promising antibacterial and antibiofilm activity against Enterococcus faecalis while maintaining acceptable cytocompatibility. When combined with calcium hydroxide, it may improve antimicrobial efficacy without compromising biocompatibility, but clinical evidence remains scarce.
Successful endodontic treatment depends on effective elimination of microorganisms from the infected root canal system and prevention of reinfection. However, complete disinfection remains difficult because of the complex anatomy of the root canal system, allowing residual microorganisms to persist and contribute to persistent apical periodontitis and treatment failure.
Minia University is the lead sponsor of 368 studies on the registry; 117 are open to participants now.
Of its 6 completed or terminated interventional studies of FDA-regulated products, 0 (0%) have results posted.
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Mandibular permanent premolar teeth:
Exclusion Criteria:
Teeth that requires further procedural steps or multidisciplinary approach, which is out of this experiment's scope:
i. Association with swelling or fistulous tract. ii. Acute periapical abscess. iii. Mobility Grade II or III. iv. Pocket depth more than 5mm. v. Previous root canal treatment. vi. Non-restorable. vii. Immature root. viii. Radiographic evidence of external or internal root resorption, vertical root fracture, perforation, calcification.
about 20 participants will be subjected to Calcium hydroxide mixed with sterile saline for intracanal placement
Procedure: Cyperus articulatus
about 20 participants will be subjected to Cyperus articulates essential oil mixed with inert gel for intracanal placement.The amount of essential oil used will be standardized for all specimens to ensure uniform consistency and reproducibility.
Procedure: Cyperus articulatus
about 20 participants will be subjected to Calcium hydroxide powder will be mixed with Cyperus articulatus essential oil using the same standardized powder-to-liquid ratio adopted in the control group until a smooth, homogeneous paste with adequate consistency is achieved.The amount of essential oil used will be standardized for all specimens to ensure uniform consistency and reproducibility.
Procedure: Cyperus articulatus
to evaluate the effect of Cyperus articulatus versus Calcium hydroxide on bacterial reduction and postoperative pain in necrotic permanent teeth with chronic apical periodontitis
Also known as: Calcium hydroxide
Anti-Microbial efficacy assessment
Assessment of The paper points of the Participants by quantitative microbiological analysis using colony-forming units (CFU/mL). and will be incubated and will be determined by bacterial counting using agar culture technique before and after root canal preparation (CFU/ml).
Time frame: 7 days
Post Operative Pain
Intensity of post- operative pain level will be recorded at 6, 12, 24, 48, 72-hours and 7 days after Endodontic treatment by numerical Rating Scale using Numerical Rate Scale (NRS) and will be used to record the patients' pain levels. The NRS (0-10 scale) where as 0 means No pain and 10 means severe pain".
Time frame: 7 days
From the registry record's own update history. This site started tracking changes on Sep 25, 2026; for anything earlier, see the record history on ClinicalTrials.gov ↗
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