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CompletedNCT07840131Updated Sep 24, 2026

Anti-microbial Effect of Acacia Arabica Extract on Gingival and Periodontal Health of Dental Patients

An interventional study of Acacia arabica Mouth Rinse and acacia arabica paste in Gingival and Periodontal Disease, sponsored by Umm Al-Qura University. Completed at 1 site in Saudi Arabia. Open to participants aged 18 Years to 45 Years. Per ClinicalTrials.gov, last updated 2026-09-24.

Sponsored by Umm Al-Qura University · Not applicable, Interventional, and Treatment

Phase
Not applicable
Study type
Interventional
Enrollment
30
Allocation
Randomized
Ages
18 Years to 45 Years
Sex
All
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Study summary

This randomized clinical pilot study evaluated the short-term effects of Acacia arabica mouth rinse and paste as adjuncts to conventional oral hygiene in adults with plaque-induced gingivitis. Thirty systemically healthy participants aged 18-42 years were randomly allocated into three groups: a control group receiving conventional oral hygiene measures, an Acacia arabica mouth rinse group, and an Acacia arabica paste group. All participants received professional plaque removal and standardized oral hygiene instructions at baseline. Participants in the intervention groups additionally used either the Acacia arabica mouth rinse or paste twice daily for four weeks. Clinical assessments were performed at baseline, 2 weeks, and 4 weeks. The study evaluated changes in plaque index, gingival index, bleeding on probing, and salivary pH. The study aimed to determine whether Acacia arabica formulations could provide additional short-term benefits in plaque and gingival inflammation control compared with conventional oral hygiene measures alone.

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Conditions studied

  • Gingival and Periodontal Disease

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Who can participate

Ages eligible
18 Years to 45 Years
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  • Adults aged 18-42 years.
  • Diagnosis of plaque-induced gingivitis.
  • Presence of gingival inflammation without clinical attachment loss or -radiographic evidence of alveolar bone loss.
  • Presence of a minimum of 15 natural teeth per arch.
  • Willingness to participate and provide written informed consent.

Exclusion criteria

Exclusion Criteria:

  • Systemic diseases or medical conditions that may affect periodontal health.
  • Recent use of antibiotics or anti-inflammatory medications.
  • Presence of fixed orthodontic appliances/wires.
  • Current smoking.
  • Pregnancy or lactation.
  • Known allergy or hypersensitivity to herbal products.
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Study design

Phase
Not applicable
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Parallel assignment
Masking
None (open label)
Enrollment
30 participants (actual)

Study arms

  • No intervention
    control

    Participants received professional plaque removal at baseline and standardized oral hygiene instructions, including the modified Bass brushing technique. Participants were instructed to brush twice daily using a regular fluoridated toothpaste and received no Acacia arabica intervention during the 4-week study period.

  • Experimental
    Acacia arabica Mouth Rinse

    Participants received professional plaque removal and standardized oral hygiene instructions at baseline. In addition, participants used 10 mL of Acacia arabica mouth rinse, prepared at a concentration of 1 g of extract per 10 mL of distilled water, twice daily after toothbrushing for 4 weeks. Participants were instructed to swish the solution for 1 minute, retain it intraorally for an additional 2 minutes, and then expectorate.

    Other: Acacia arabica Mouth Rinse

  • Experimental
    Acacia arabica Paste

    Participants received professional plaque removal and standardized oral hygiene instructions at baseline. In addition, participants applied a pea-sized amount of Acacia arabica paste (Gumtone® gel) to the gingiva twice daily after toothbrushing for 4 weeks. The paste was gently massaged into the gingiva for approximately 2 minutes and subsequently removed using gauze.

    Other: acacia arabica paste

Interventions

  • OtherAcacia arabica Mouth Rinse

    the rinse as 1 g extract/10 mL distilled water

  • Otheracacia arabica paste

    a pea-sized amount of Acacia arabica paste (Gumtone® gel)

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What researchers measure

Primary outcomes

  1. Change in Gingival Index (GI)

    Gingival inflammation was assessed using the Gingival Index at baseline, 2 weeks, and 4 weeks. The index was used to evaluate changes in the severity of gingival inflammation during the intervention period. Lower scores indicate less gingival inflammation and better gingival health.

    Time frame: Baseline, 2 weeks, and 4 weeks

  2. Change in Plaque Index (PI)

    Plaque accumulation was assessed using the Plaque Index at baseline, 2 weeks, and 4 weeks. The index was used to quantify changes in dental plaque accumulation during the intervention period. Lower scores indicate less plaque accumulation and better plaque control.

    Time frame: Baseline, 2 weeks, and 4 weeks

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Study locations

1 site
  • Umm AlQuar University Dental Hospital
    Mecca, Saudi Arabia
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References and documents

Publications

  • Pradeep AR, Happy D, Garg G. Short-term clinical effects of commercially available gel containing Acacia arabica: a randomized controlled clinical trial. Aust Dent J. 2010 Mar;55(1):65-9. doi: 10.1111/j.1834-7819.2009.01180.x. PubMed 20415914 ↗

Study documents

  • Protocol and informed consent form · Jan 1, 2025
  • Protocol and statistical analysis plan · Jan 1, 2025

Documents are hosted by the registry — open the source record to download them.

Individual participant data

Plan to share: No — Individual participant data are not planned to be publicly shared due to participant confidentiality and institutional ethical considerations. De-identified data supporting the findings may be made available from the corresponding investigator upon reasonable request and subject to institutional and ethical approval.

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Registry details

Key details

Study ID
NCT07840131
Lead sponsor
Umm Al-Qura University
Responsible party
Elham Alsahafi (Associate Professor, Umm Al-Qura University) — Principal investigator
First posted
Sep 24, 2026
Start date
Jan 1, 2025
Primary completion
Aug 31, 2025
Completion
Dec 30, 2025
Last update
Sep 24, 2026

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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This study is completed, as verified in Aug 2026. You cannot join it, but the record below documents what was studied.

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