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Active, not recruitingNCT07821086Updated Sep 15, 2026

Effects of Estrogen Therapy on Lipid Metabolism and Insulin Sensitivity in Hypogonadal Women

An interventional study of Estrogen in Female Hypogonadism, sponsored by Khyber Medical University Peshawar. Active, not recruiting at 1 site in Pakistan. Open to female participants aged 18 Years to 50 Years. Per ClinicalTrials.gov, last updated 2026-09-15.

Sponsored by Khyber Medical University Peshawar · Not applicable, Interventional, and Treatment

Phase
Not applicable
Study type
Interventional
Enrollment
30
Allocation
Not applicable
Ages
18 Years to 50 Years
Sex
Female
01

Study summary

This study aims to evaluate the short-term effects of estrogen therapy on lipid metabolism and insulin sensitivity in hypogonadal women. Participants receiving estrogen therapy will be assessed before initiation of treatment and after the treatment period to determine changes in serum estrogen levels, fasting glucose, fasting insulin, and lipid profile. Insulin sensitivity will be assessed using the HOMA-IR index. The findings may contribute to a better understanding of the short-term metabolic effects of estrogen therapy in women with hypogonadism.

Read the detailed description

Female hypogonadism is characterised by reduced ovarian function and estrogen deficiency and may be associated with metabolic alterations, including disturbances in lipid metabolism and insulin sensitivity. Estrogen therapy is used to restore estrogen levels in women with estrogen deficiency; however, the short-term metabolic effects of estrogen therapy in women with hypogonadism require further evaluation.

This single-group pre-post experimental study will evaluate changes in lipid metabolism and insulin sensitivity among hypogonadal women receiving oral estrogen therapy. Eligible participants will undergo baseline assessment before initiation of estrogen therapy and will be reassessed following the treatment period. Serum estrogen levels, fasting glucose, fasting insulin, and lipid profile, including total cholesterol, LDL-C, HDL-C, and triglycerides, will be measured at baseline and follow-up. Insulin sensitivity will be assessed using the Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) index.

The study will assess changes in these metabolic parameters following estrogen therapy and provide evidence regarding its short-term effects on lipid metabolism and glucose-insulin regulation in hypogonadal women. The findings may contribute to a better understanding of the metabolic response to estrogen therapy and inform future research on the management of metabolic risks associated with estrogen deficiency.

02

Conditions studied

  • Female Hypogonadism

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Keywords

  • Female Hypogonadism
  • Lipid Metabolism
  • Estrogen Therapy
  • Insulin Sensitivity
  • Insulin Resistance
  • Lipid Profile
  • Estrogen Deficiency
03

Who can participate

Ages eligible
18 Years to 50 Years
Sexes eligible
Female
Accepts healthy volunteers
No

Inclusion criteria

  • Women aged 18 to 50 years.
  • Diagnosis of hypogonadism, including:
  • Primary ovarian insufficiency (POI)
  • Hypogonadotropic hypogonadism
  • Early menopause

Exclusion criteria

Exclusion Criteria:

  • Contraindications to estrogen therapy, including a history of thromboembolic events or certain hormone-sensitive cancers.
  • Pre-existing conditions that may significantly affect lipid metabolism or insulin sensitivity.
  • Use of medications known to affect lipid metabolism or insulin sensitivity.
  • Pregnancy or lactation.
04

Study design

Phase
Not applicable
Primary purpose
Treatment
Allocation
Not applicable
Intervention model
Single group
Masking
None (open label)
Enrollment
30 participants (actual)

Study arms

  • Experimental
    Estrogen therapy

    Participants with hypogonadism who have not previously started estrogen therapy will receive estrogen therapy as prescribed by their treating healthcare professional. Participants will undergo assessment at baseline before initiation of therapy and again after 3 months of treatment. Changes in lipid profile parameters (total cholesterol, LDL-C, HDL-C, and triglycerides) and insulin sensitivity, assessed using fasting glucose, fasting insulin, and HOMA-IR, will be evaluated between baseline and 3 months. Adverse events and treatment-related complications will also be recorded during the follow-up period.

    Drug: Estrogen

Interventions

  • DrugEstrogen

    strogen therapy will be administered to women with hypogonadism as prescribed by a healthcare professional. Estrogen may be administered using oral or transdermal formulations at the prescribed dosage. Participants will receive therapy for 3 months, with assessments performed at baseline before initiation of therapy and after 3 months of treatment. The effects of estrogen therapy on lipid metabolism and insulin sensitivity will be evaluated using lipid profile parameters, fasting glucose, fasting insulin, and HOMA-IR. Serum estrogen levels will also be assessed during follow-up, and adverse events or treatment-related complications will be recorded.

    Also known as: Estradiol Valerate ( 2 Mg)

05

What researchers measure

Primary outcomes

  1. Change in Lipid Profile

    Change in total cholesterol, LDL-C, HDL-C, and triglyceride levels from baseline (before initiation of estrogen therapy) to 3 months after initiation of estrogen therapy.

    Time frame: Baseline to 3 Months

  2. Change in Insulin Sensitivity (HOMA-IR) from Baseline to 3 Months

    Change in insulin resistance estimated using the HOMA-IR index from baseline (before initiation of estrogen therapy) to 3 months after initiation of estrogen therapy. HOMA-IR will be calculated from fasting glucose and fasting insulin measurements.

    Time frame: Baseline to 3 Months

Secondary outcomes

  1. Change in a Serum Estrogen Level

    Change in serum estrogen levels from baseline to 3 months following initiation of estrogen therapy.

    Time frame: Baseline to 3 Months

06

Study locations

1 site
  • Institute of Basic Medical Sciences
    Peshawar, Khyber Pakhtunkhwa 25100, Pakistan
07

References and documents

Publications

  • Tebbens M, Schutte M, Troelstra MA, Bruinstroop E, de Mutsert R, Nederveen AJ, den Heijer M, Bisschop PH. Sex Steroids Regulate Liver Fat Content and Body Fat Distribution in Both Men and Women: A Study in Transgender Persons. J Clin Endocrinol Metab. 2023 Dec 21;109(1):e280-e290. doi: 10.1210/clinem/dgad409. PubMed 37463488 ↗
  • Dam TV, Dalgaard LB, Thomsen CB, Hjortebjerg R, Ringgaard S, Johansen FT, Bengtsen MB, Mose M, Lauritsen KM, Sondergaard E, Gravholt CH, Hansen M. Estrogen modulates metabolic risk profile after resistance training in early postmenopausal women: a randomized controlled trial. Menopause. 2021 Nov 1;28(11):1214-1224. doi: 10.1097/GME.0000000000001841. PubMed 34726661 ↗
  • Huang L, Wang H, Shi M, Kong W, Jiang M. Lipid Profile in Patients With Primary Ovarian Insufficiency: A Systematic Review and Meta-Analysis. Front Endocrinol (Lausanne). 2022 Jun 10;13:876775. doi: 10.3389/fendo.2022.876775. eCollection 2022. PubMed 35757391 ↗

Individual participant data

Plan to share: Yes — De-identified individual participant data underlying the results reported in the study, including demographic and clinical characteristics and laboratory measurements related to lipid profile, fasting glucose, fasting insulin, HOMA-IR, and serum estrogen levels, may be shared with qualified researchers for scientifically valid research purposes.

Supporting information: Study protocol, Sap, Analytic code

08

Registry details

Key details

Study ID
NCT07821086
Lead sponsor
Khyber Medical University Peshawar
Responsible party
Sponsor
First posted
Sep 15, 2026
Start date
Dec 4, 2025
Primary completion
Jul 9, 2026
Completion
Sep 20, 2026 (estimated)
Last update
Sep 15, 2026

Study contacts

Mona Jamal, MPhil
principal investigator · Khyber Medical University Peshawar
Mohsin Shah, PhD
study director · Khyber Medical University Peshawar

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

Not currently enrolling

This study is active, not recruiting, as verified in Sep 2026. You cannot join it, but the record below documents what was studied.

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