An interventional study of Anthocyanin Group and Placebo Group in Supplement, sponsored by Kocatepe University. Completed at 1 site in Turkey (Türkiye). Open to male participants aged 18 Years to 27 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-08-20.
Sponsored by Kocatepe University · Not applicable, Interventional, and Other
his randomized, placebo-controlled trial investigated whether black elderberry (Sambucus nigra L.) supplementation affects blood lactate responses, oxidative stress biomarkers, and exercise-related physiological responses in young men who regularly exercise. Participants completed four assessment sessions: a pre-test and three supplementation phases involving a single dose, alternate-day supplementation for 14 days, and daily supplementation for 14 days. The anthocyanin group received black elderberry supplementation, while the control group received an identical placebo. Blood lactate, oxidative stress biomarkers, body composition, and exercise performance were assessed under standardized laboratory conditions across the study phases.
Kocatepe University is the lead sponsor of 7 studies on the registry; none are open to participants now.
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Exclusion Criteria:
Underlined terms in the criteria open these too. Explained by clinicaltrials.gg, not part of the registry record. The study team decides who can take part.
Before you join, the study team explains what taking part involves — its purpose, what happens, the risks and possible benefits — and answers your questions, so you can decide freely. Consent is usually recorded on a form (the informed consent form, ICF) signed by you or, where the law allows, by someone legally authorised to decide for you.
You can keep asking questions during the study, you are told of new information that matters, and you can choose to leave at any time.
Informed consent
Before you join, the study team explains what taking part involves — its purpose, what happens, the risks and possible benefits — and answers your questions, so you can decide freely. Consent is usually recorded on a form (the informed consent form, ICF) signed by you or, where the law allows, by someone legally authorised to decide for you.
You can keep asking questions during the study, you are told of new information that matters, and you can choose to leave at any time.
Explained by clinicaltrials.gg, not part of the registry record. The study team decides who can take part.
Participants received black elderberry (Sambucus nigra L.) supplementation according to the study protocol, including single-dose, alternate-day, and daily supplementation phases.
Dietary Supplement: Anthocyanin Group
Participants received placebo capsules identical in appearance and administration schedule to the black elderberry capsules.
Dietary Supplement: Placebo Group
Participants received 300 mg black elderberry (Sambucus nigra L.) extract capsules according to the study protocol.
Participants received placebo capsules containing maltodextrin according to the study protocol.
Catalase (CAT) Activity
Plasma catalase (CAT) activity was assessed at the pre-test and at the end of each supplementation phase. At the pre-test, venous blood was collected before the exercise protocol. During each supplementation phase, venous blood was collected 15 minutes after completion of the exercise protocol. In each supplementation phase, the final dose was administered approximately 2 hours before the exercise test. CAT activity was measured using a commercial ELISA kit (BT-LAB, Bioassay Technology Laboratory, Zhejiang, China) and reported in nmol/min/mL.
Time frame: Pre-test (before the exercise protocol) and 15 minutes after the exercise protocol at the end of each supplementation phase (single-dose, 14-day alternate-day, and 14-day daily supplementation).
Glutathione (GSH) Level
Plasma glutathione (GSH) level was assessed at the pre-test and at the end of each supplementation phase. At the pre-test, venous blood was collected before the exercise protocol. During each supplementation phase, venous blood was collected 15 minutes after completion of the exercise protocol. In each supplementation phase, the final dose was administered approximately 2 hours before the exercise test. GSH level was measured using a commercial ELISA kit (BT-LAB, Bioassay Technology Laboratory, Zhejiang, China) and reported in µmol/g
Time frame: Pre-test (before the exercise protocol) and 15 minutes after the exercise protocol at the end of each supplementation phase (single-dose, 14-day alternate-day, and 14-day daily supplementation).
Malondialdehyde (MDA)
Plasma malondialdehyde (MDA) concentration was assessed at the pre-test and at the end of each supplementation phase. At the pre-test, venous blood was collected before the exercise protocol. During each supplementation phase, venous blood was collected 15 minutes after completion of the exercise protocol. In each supplementation phase, the final dose was administered approximately 2 hours before the exercise test. MDA concentration was measured using a commercial ELISA kit (BT-LAB, Bioassay Technology Laboratory, Zhejiang, China) and reported in nmol/mL.
Time frame: Pre-test (before the exercise protocol) and 15 minutes after the exercise protocol at the end of each supplementation phase (single-dose, 14-day alternate-day, and 14-day daily supplementation).
Superoxide Dismutase (SOD) Activity
Plasma superoxide dismutase (SOD) activity was assessed at the pre-test and at the end of each supplementation phase. At the pre-test, venous blood was collected before the exercise protocol. During each supplementation phase, venous blood was collected 15 minutes after completion of the exercise protocol. In each supplementation phase, the final dose was administered approximately 2 hours before the exercise test. SOD activity was measured using a commercial ELISA kit (BT-LAB, Bioassay Technology Laboratory, Zhejiang, China) and reported in U/mL.
Time frame: Pre-test (before the exercise protocol) and 15 minutes after the exercise protocol at the end of each supplementation phase (single-dose, 14-day alternate-day, and 14-day daily supplementation).
Nitric Oxide (NO)
Plasma nitric oxide (NO) concentration was assessed at the pre-test and at the end of each supplementation phase. At the pre-test, venous blood was collected before the exercise protocol. During each supplementation phase, venous blood was collected 15 minutes after completion of the exercise protocol. In each supplementation phase, the final dose was administered approximately 2 hours before the exercise test. NO concentration was measured using a commercial ELISA kit (BT-LAB, Bioassay Technology Laboratory, Zhejiang, China) and reported in µmol/L.
Time frame: Pre-test (before the exercise protocol) and 15 minutes after the exercise protocol at the end of each supplementation phase (single-dose, 14-day alternate-day, and 14-day daily supplementation).
Blood Lactate Concentration
Blood lactate concentration measured from capillary blood samples using a portable lactate analyzer (EDGE).
Time frame: Baseline (pre-test), during the 3rd minute of exercise, immediately post-exercise, and 30 minutes post-exercise during recovery; measurements repeated after Baseline (pre-test), single-dose, alternate-day, and daily supplementation phases.
Plan to share: No — Individual participant data will not be shared with other researchers to protect participants' privacy and confidentiality. The findings of the study will be reported only in aggregated and anonymized form.
No publications or documents are linked to this record.
Ran from May 2024 to primary completion in Jul 2024, 2 months.
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Studies of the drug tested here: Anthocyanins
Kocatepe University