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Not yet recruitingNCT07757828Updated Aug 11, 2026

Efficacy of PRF in Hypospadias Repair

An interventional study of Tubularized Incised Plate urethroplasty with intraoperative placement of autologous PRF onto the urethral plate. and standard Tubularized Incised Plate urethroplasty in Hypospadias, sponsored by Ain Shams University. Not yet recruiting at 1 site in Egypt. Open to male participants aged 6 Months to 18 Years. Per ClinicalTrials.gov, last updated 2026-08-11.

Sponsored by Ain Shams University · Not applicable, Interventional, and Treatment

Phase
Not applicable
Study type
Interventional
Enrollment
80
Allocation
Randomized
Ages
6 Months to 18 Years
Sex
Male
01

Study summary

Hypospadias is an anomaly characterized by an ectopic ventral urethral meatus, and an incomplete or hooded prepuce. Its incidence is estimated at 1 in 200-300 live male births, with variations across populations and severity types.

Successful hypospadias repair depends largely on optimal tissue healing and robust vascular support around the neourethra. Thus, many adjunctive techniques have been investigated to improve outcomes, including dartos flaps, tunica vaginalis flaps, and various tissue sealants.

In recent years, attention has turned toward biological augmentation strategies, particularly the use of platelet-rich plasma (PRP) and platelet-rich fibrin as a potential enhancer of wound healing.

Platelet-rich fibrin (PRF), a second-generation autologous platelet concentrate, has recently emerged as another promising biologic in tissue engineering.

This study aims to evaluate the effect of PRF application in primary hypospadias repair surgery and determine its effect in improving healing and reduction of postoperative complications.

Read the detailed description

All patients included in the study will undergo primary hypospadias repair using the standardized Tubularized Incised Plate (TIP) urethroplasty technique (Snodgrassi procedure), under general anesthesia and by the same surgical team to minimize variability

  • Steps of the Surgical Procedure (Same for Both Groups):

    1. Preoperative preparation:

  • IV antibiotics, sterile field setup, general anesthesia. 2. Urethral plate incision and tubularization over a 6-8 Fr stent using absorbable sutures.

    3. application of PRF (Group A only):

  • 8-10 mL of autologous blood is drawn into plain (non-anticoagulant) tubes according to the patient's weight and the size of the patch needed.
  • The sample is immediately centrifuged using the single-spin method (typically 3,000 rpm (400x g) for 12 minutes).
  • This produces three layers:

    • Top layer: acellular plasma (PPP)
    • Middle layer: platelet-rich fibrin (PRF) clot
    • Bottom layer: red blood cells The PRF clot is gently removed and compressed using a gauze to form a thin PRF membrane.
  • PRF membrane is fixed on top of the suture line as a first layer and under the incision line as a second layer and secured using a few resorbable polyglyconate 6/0 interrupted sutures.

    4. Glansplasty and skin closure using standard techniques in both groups 5. Catheter and dressing:

  • A soft catheter is left in place for 5-7 days.
  • A mild compression dressing is applied.
02

Conditions studied

  • Hypospadias

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Keywords

  • Hypospadias
  • PRF
  • Repair
03

Who can participate

Ages eligible
6 Months to 18 Years
Sexes eligible
Male
Accepts healthy volunteers
No

Inclusion criteria

  • Diagnosed with primary distal or mid-penile hypospadias.
  • Eligible for first-time (primary) surgical repair using the TIP (Tubularized Incised Plate) technique.

Exclusion criteria

Exclusion Criteria:

  • previous hypospadias surgery.
  • Associated severe chordee requiring staged repair or patient with crippled hypospadias.
  • Children with systemic diseases that may impair wound healing (e.g., diabetes, immunodeficiency, malnutrition).
  • Presence of bleeding or coagulation disorders.
  • Medically Unfit for surgery.
04

Study design

Phase
Not applicable
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Single (Participant)
Enrollment
80 participants (estimated)

Study arms

  • Experimental
    Group A TIP urethroplasty with autologous PRF

    Undergoes TIP urethroplasty with intraoperative placement of autologous PRF onto the urethral plate.

    Procedure: Tubularized Incised Plate urethroplasty with intraoperative placement of autologous PRF onto the urethral plate.

  • Active comparator
    Group B standard TIP urethroplasty without PRF

    Undergoes standard TIP urethroplasty without PRF application

    Procedure: standard Tubularized Incised Plate urethroplasty

Interventions

  • ProcedureTubularized Incised Plate urethroplasty with intraoperative placement of autologous PRF onto the urethral plate.

    Urethral plate incision and tubularization over a 6-8 Fr stent using absorbable sutures. Application of PRF where 8-10 mL of autologous blood is drawn into plain (non-anticoagulant) tubes and a thin PRF membrane is extracted which is fixed on top of the suture line as a first layer and under the incision line as a second layer and secured using a few resorbable polyglyconate 6/0 interrupted sutures. Glansplasty and skin closure using standard techniques and fixing catheter and dressing

  • Procedurestandard Tubularized Incised Plate urethroplasty

    Urethral plate incision and tubularization over a 6-8 Fr stent using absorbable sutures. Glansplasty and skin closure using standard techniques and fixing catheter and dressing

05

What researchers measure

Primary outcomes

  1. Urethrocutaneous fistula formation

    Incidence of urethrocutaneous fistula formation

    Time frame: 6 months

  2. Parental satisfaction Assessment

    Parental satisfaction assessed using a standardized questionnaire HOPE score

    Time frame: 6 months

06

Study locations

1 site
  • Ain Shams University
    Cairo, Abbasia 11517, Egypt
07

References and documents

Publications

  • Eryilmaz R, Simsek M, Aslan R, Beger B, Ertas K, Taken K. The effect of plasma rich platelet graft on post-operative complications in mid-penile hypospadias. Andrologia. 2020 Aug;52(7):e13652. doi: 10.1111/and.13652. Epub 2020 May 20. PubMed 32436309 ↗

Individual participant data

Plan to share: No

08

Registry details

Key details

Study ID
NCT07757828
Lead sponsor
Ain Shams University
Responsible party
Younan Ramsis (Lecturer of urology, Ain Shams University) — Principal investigator
First posted
Aug 11, 2026
Start date
Aug 1, 2026 (estimated)
Primary completion
Aug 1, 2027 (estimated)
Completion
Oct 1, 2027 (estimated)
Last update
Aug 11, 2026

Study contacts

Mahmoud Mohamed Magdy El-Ansary, MSc
Contact
mahmoudmagdy99@hotmail.com
+201114393349
Younan R. Samir, M.D.
Contact
dr_yona@live.com
+201201255511
Hany H. Gad, Professor
study chair · Ain Shams University
Hossam M.H. ElAwady, Professor
study director · Ain Shams University

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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