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RecruitingNCT07720414Updated Jul 22, 2026

Effects of GLP-1 RAs on Human Lactation Outcomes

An observational study in Lactation and Weight Loss, sponsored by University of Colorado, Denver. Recruiting at 1 site in United States. Open to female participants, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-07-22.

Sponsored by University of Colorado, Denver · Observational

Study type
Observational
Model
Cohort
Time perspective
Prospective
Enrollment
18
Sex
Female
01

Study summary

There is increasing interest in using GLP-1 Receptor Agonist (GLP-1 RA) medications after pregnancy. This study is designed to determine if these medications affect breastmilk production or the nutritional makeup of breastmilk.

The main questions the study will address are:

  • Does GLP-1 RA-induced weight loss increase or decrease breastmilk production?
  • Does GLP-1 RA-induced weight loss affect the nutritional composition of milk, including milk fat content?

Researchers will assess milk production rates before and after mothers begin taking injected GLP-1 RA medications to decrease their body weight. Weight loss will be confirmed with 7-day body weight averages.

Participants will:

  • Weigh themselves and report measurements daily.
  • Participate in 2-3 morning study visits over approx. 2.5-4.5 months (approx. 3-3.5hrs each).

During each visit, participants will:

  • Use a study provided breastpump to express milk 3 times hourly for measurement of hourly milk production.
  • Provide 1-3 oz of milk for further study.
  • Answer questions about breastfeeding patterns.
02

Conditions studied

  • Lactation
  • Weight Loss

Keywords

  • Breastmilk production
  • Breastmilk composition
  • GLP-1
  • Weight Loss
03

Who can participate

Ages eligible
Child (0–17), Adult (18–64), Older adult (65+)
Sexes eligible
Female
Accepts healthy volunteers
Yes
Sampling method
Non-probability sample

Study population

Breastfeeding mothers willing to attend study study visits in Aurora, CO.

Inclusion criteria

  • Breastfeeding mothers of single or multiple infants.
  • Milk removal at least 4x daily
  • Eligible for a GLP-1 RA administered subcutaneously (oral GLP-1 RAs contain absorption enhancers, which may drive accumulation in milk)
  • Access to a breastpump, if needed for maintaining breastfeeding behaviors.
  • Willingness to provide 1-3 oz of breastmilk per study visit.
  • Willingness to track and report body weight daily.
  • Willingness to maintain breastfeeding behaviors for the duration of the study.

Exclusion criteria

Exclusion Criteria:

  • Currently taking GLP-1 RA medication or other weight loss medication
  • Induced lactation, due to unknowns regarding the durability of the mammary gland's milk production after exogenous stimulation.
04

Study design

Observational model
Cohort
Time perspective
Prospective
Enrollment
18 participants (estimated)
Patient registry
No
Biospecimen retention
Samples with dna

Groups and cohorts

  • Breastfeeding mothers prescribed GLP-1 RA therapies

    Breastfeeding mothers who have been prescribed, but have not started, an injected GLP-1RA (these patients have an indication for GLP-1 RA treatment (BMI ≥27 kg/m2 with at least one comorbidity (prediabetes, diabetes, metabolic syndrome, obstructive sleep apnea, hypertension, dyslipidemia, cardiovascular disease, heart failure, chronic kidney disease, metabolic dysfunction-associated steatohepatitis, atrial fibrillation, venous thromboembolic disease, osteoarthritis, polyendocrine metabolic ovarian syndrome), or BMI ≥30 kg/m2)) and who are committed to sustaining breastfeeding habits (frequency and volume of milk removal) for the duration of the study period.

    Drug: Injected GLP-1 Receptor Agonists and Dual Agonists

Interventions

  • DrugInjected GLP-1 Receptor Agonists and Dual Agonists

    Participants are prescribed this medication by their care provider(s) as part of their postnatal clinical care, including but not limited to semaglutide and tirzepatide. The investigators do not provide, manage, or assign the medication.

05

What researchers measure

Primary outcomes

  1. Hourly milk production rate

    Milk production rate (in grams per hour) will be assessed using a standardized milk expression protocol.

    Time frame: From one week before until a maximum of 2 months after beginning GLP-1RA treatment. This timeframe will restart if their dosage is increased.

  2. Milk macronutrient composition

    Milk macronutrient composition will be assessed using the Miris Human Milk Analyzer.

    Time frame: From one week before until a maximum of 2 months after beginning GLP-1RA treatment. This timeframe will restart if the dosage is increased.

06

Study locations

1 of 1 sites recruiting
  • University of Colorado Anschutz School of Medicine
    Aurora, Colorado 80045, United States
    Recruiting
07

References and documents

Individual participant data

Plan to share: Yes — De-identified individual participant data and leftover biological samples may be deposited into public or restricted-access data banks, or distributed directly to external researchers for future study without additional participant consent. Data and specimens will be stripped of names and direct identifiers prior to sharing or repository submission. Recipients of these assets may include researchers working at other universities, government agencies, or private industry. Future research utilizing this data may focus on obesity, weight loss treatments, or other related medical conditions. While data are de-identified to protect privacy, absolute protection against re-identification cannot be guaranteed, and broad data sharing may involve unknown risks.

No publications or documents are linked to this record.

08

Registry details

Key details

Study ID
NCT07720414
Lead sponsor
University of Colorado, Denver
Responsible party
Sponsor
First posted
Jul 22, 2026
Start date
Jul 2026 (estimated)
Primary completion
Jun 2028 (estimated)
Completion
Jun 2028 (estimated)
Last update
Jul 22, 2026

Study contacts

Jayne F Martin Carli, PhD
Contact
jayne.martincarli@cuanschutz.edu
303-724-3981
Jacinda M Nicklas, MD, MPH
Contact
jacinda.nicklas@cuanschutz.edu
303-724-9028

Oversight

Data monitoring committee
No
FDA-regulated drug
Yes
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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