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RecruitingNCT07691749Updated Sep 30, 2026

Effect of Psychobiotics With Standard Antiddepressants in Treatment of Major Depressive Disorder

An interventional study of Lactobacillus Helveticus and bifidobacterium Longum and Placebo Capsule(s) in Major Depression, sponsored by Dr Sadia yasir. Recruiting at 1 site in Pakistan. Open to participants aged 18 Years to 60 Years. Per ClinicalTrials.gov, last updated 2026-09-30.

Sponsored by Dr Sadia yasir · Not applicable, Interventional, and Treatment

Phase
Not applicable
Study type
Interventional
Enrollment
86
Allocation
Randomized
Ages
18 Years to 60 Years
Sex
All
01

Study summary

The goal of this clinical trial is to learn whether adding psychobiotics (a combination of Lactobacillus helveticus and Bifidobacterium longum) to standard antidepressant treatment works better than antidepressants alone for adults with major depressive disorder. The study will also learn about the safety of taking these psychobiotics with antidepressants.

The main questions it aims to answer are:

  1. Does adding psychobiotics lower depression symptoms more than a placebo (a look-alike capsule with no active ingredients)?
  2. Is it safe and well tolerated when taken with standard antidepressant treatment?

Researchers will compare psychobiotic capsules with placebo capsules to see whether psychobiotics improve depression symptoms when used together with standard antidepressants.

Participants will:

  1. Continue taking their prescribed antidepressant medicine. Be randomly assigned to receive either a psychobiotic capsule or a placebo capsule once daily for 6 weeks.
  2. Complete depression assessments at the start of the study and again at 3 and 6 weeks.
  3. Attend regular follow-up visits so researchers can monitor symptoms, side effects, and overall progress
Read the detailed description

Major depressive disorder (MDD) is a leading cause of disability worldwide, and many patients experience only partial improvement with antidepressant treatment alone. Increasing evidence suggests that the gut-brain axis plays an important role in the pathophysiology of depression. Psychobiotics, including Lactobacillus helveticus and Bifidobacterium longum, may improve depressive symptoms by modulating the gut microbiota, reducing inflammation, and influencing neurochemical pathways.

This study is a randomized, double-blind, placebo-controlled trial designed to evaluate the efficacy and safety of adjunctive psychobiotic therapy in adults with MDD receiving standard antidepressant treatment. Participants will continue their prescribed antidepressant medication and will be randomly assigned to receive either a psychobiotic capsule containing Lactobacillus helveticus and Bifidobacterium longum or a matching placebo once daily for 6 weeks.

Depression severity will be assessed at baseline, Week 3, and Week 6 using validated rating scales. Safety and tolerability will be evaluated through adverse event monitoring, treatment adherence, and regular follow-up visits.

The primary objective is to determine whether adjunctive psychobiotic therapy produces a greater reduction in depressive symptoms compared with placebo. Secondary objectives include evaluating safety, tolerability, treatment adherence, patient satisfaction, and quality of life. The findings will provide evidence on whether psychobiotics are an effective and safe adjunct to antidepressant therapy in adults with major depressive disorder.

02

Conditions studied

  • Major Depression

Keywords

  • Psychobiotics
  • Major Depressive Disorder
  • Lactobacillus Helveticus
  • Bifidobacterium longum
  • Gut-Brain Axis
  • Selective Serotonin Reuptake Inhibitors (SSRIs)
  • Adjunctive Therapy
  • Placebo-Controlled Trial
  • Hamilton Depression Rating Scale (HDRS-17)
03

Who can participate

Ages eligible
18 Years to 60 Years
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  1. Patients diagnosed with major depressive disorder (According to Diagnostic \& Statistical Manual-5 criteria (as per operational definition)
  2. Age 18 to 60 years
  3. Both genders
  4. Patients who are able to speak and understand Urdu/ English language.
  5. Patients who are on stable doses of oral selective serotonin reuptake inhibitors for at least two weeks prior to enrollment in the study
  6. Patients with cut off score of > 24 as assessed by 17 item clinician administered Hamilton depression rating scale.

Exclusion criteria

Exclusion Criteria:

  • Severe psychiatric comorbidities (schizophrenia, bipolar disorder, co morbid substance use)

    • Individuals with weakened immune systems: This includes patients undergoing chemotherapy, transplant recipients, and people with conditions like HIV/AIDS.
    • Individuals on chronic medication: People on chronic pharmacological therapy, especially with anti-inflammatory drugs or who have recently used antibiotics in last 2 weeks.
    • Pregnant or lactating women
    • Current use of probiotics or prebiotics
04

Study design

Phase
Not applicable
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Triple (Participant, Investigator, Outcomes assessor)
Enrollment
86 participants (estimated)

Study arms

  • Active comparator
    Interventional Arm

    People who are recieving Psychobiotics as an adjunct to the standard treatment.

    Dietary Supplement: Lactobacillus Helveticus and bifidobacterium Longum

  • Placebo comparator
    Placebo Comparator

    people who will be given placebo capsules along with standard treatment

    Other: Placebo Capsule(s)

Interventions

  • Dietary supplementLactobacillus Helveticus and bifidobacterium Longum

    This study uses a specific combination of two well-studied psychobiotic strains-Lactobacillus helveticus R0052 (1.8 billion CFU) and Bifidobacterium longum R0175 (1.2 billion CFU). These strains have been selected because they may support communication between the gut and the brain and help improve mood when used alongside standard antidepressant treatment.

    Also known as: psychobiotics

  • OtherPlacebo Capsule(s)

    A placebo capsule is an orally administered capsule formulated with inert gelatin and no active ingredients. It has no psychobiotic potential and is pharmacologically inactive. The capsule is not expected to affect study outcomes or interact with oral selective serotonin reuptake inhibitors (SSRIs)

05

What researchers measure

Primary outcomes

  1. Change in Hamilton Depression Rating Scale-17 (HDRS-17) Total Score

    The primary outcome is the difference in the mean change in the 17-item Hamilton Depression Rating Scale (HDRS-17) total score from baseline to Week 6 between participants receiving psychobiotics (Lactobacillus helveticus R0052 and Bifidobacterium longum R0175) plus standard antidepressant treatment and those receiving placebo plus standard antidepressant treatment. Higher reductions in HDRS-17 scores indicate greater improvement in depressive symptoms.

    Time frame: Baseline to Week 6

06

Study locations

1 of 1 sites recruiting
  • Allama Iqbal Medical College, Jinnah Hospital Lahore
    Lahore, Punjab Province 54550, Pakistan
    • Head Of Department, PSYCHIATRY DEPARTMENT · Contact · psychjhl@aimc.edu.pk · +923334746639
    • AROOJ FATIMA, MBBS, MD PSY · Sub investigator
    • ZAINAB MAHMOOD, MBBS, FCPS · Sub investigator
    • TEHREEM KHAN, MBBS · Sub investigator
    • TARIQ ABBAS, MBBS · Sub investigator
    • MOHSIN ALI, MBBS · Sub investigator
    • TAYYAB RAFIQUE, MBBS · Sub investigator
    Recruiting
07

References and documents

Study documents

  • Protocol, analysis plan and consent form · Feb 11, 2026

Documents are hosted by the registry — open the source record to download them.

Individual participant data

Plan to share: No

08

Registry details

Key details

Study ID
NCT07691749
Lead sponsor
Dr Sadia yasir
Collaborators
Allama Iqbal Medical College, Jinnah Hospital
Responsible party
Dr Sadia yasir (Associate Professor/ Head of Department, Jinnah Hospital) — Sponsor-investigator
First posted
Jul 9, 2026
Start date
Jan 31, 2026
Primary completion
Dec 25, 2026 (estimated)
Completion
Jan 31, 2027 (estimated)
Last update
Sep 30, 2026

Study contacts

Arooj Fatima Senior Registrar, Psychiatry Department, MBBS, MD Psychiatry
Contact
aroojfatima47@yahoo.com
+923314567690
Arooj Fatima, MBBS, MD Psychiatry
Contact
aroojfatima47@yahoo.com
+923314567690
Sadia Yasir, MBBS FCPS
study chair · Jinnah hospital lahore/ Allama Iqbal Medical college lahore, Pakistan
Arooj Fatima
principal investigator · Jinnah Hospital

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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