An interventional study of Casein Protein Isolate Drink and Whey Protein Isolate Drink in Aging and Protein Metabolism, sponsored by National University of Singapore. Active, not recruiting at 1 site in Singapore. Open to participants aged 60 Years to 80 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-02-17.
Sponsored by National University of Singapore · Not applicable, Interventional, and Prevention
The study aims to investigate the acute effects of different protein sources (i.e., casein, whey, soy) on metabolomic profiles (e.g., amino acids in the blood), along with other outcomes such as cognitive function, mood, and appetite. While the study does not directly aim to prevent, prognosticate, diagnose, or alleviate any disease, it may provide insights that could inform dietary recommendations and support disease prevention efforts in older adults.
This study will be a double-blind, randomised, placebo-controlled trial using a crossover study design. 25 older adults (aged 60 to 80) will be recruited and assigned to consume 4 different test drinks in a random order on 4 different visits. This study aims to investigate whether higher protein consumption of different sources will influence postprandial plasma and urinary metabolomic profiles, cognition, mood and appetite. The study will comprise 1 screening visit and 4 test visits separated separated by a minimum washout period of >7 days
National University of Singapore is the lead sponsor of 206 studies on the registry; 52 are open to participants now.
Counted across the registry records on this site, refreshed daily.
Exclusion Criteria:
Currently on a specialised diet (e.g., vegetarian, vegan, weight loss diet)
30 g protein equivalent of casein protein isolate dissolved in \~250 mL of water
Dietary Supplement: Casein Protein Isolate Drink
30 g protein equivalent of whey protein isolate dissolved in \~250 mL of water
Dietary Supplement: Whey Protein Isolate Drink
30 g protein equivalent of soy protein isolate dissolved in \~250 mL of water
Dietary Supplement: Soy Protein Isolate Drink
30 g carbohydrate equivalent of maltodextrin powder dissolved in \~250 mL of water
Dietary Supplement: Maltodextrin Drink
30 g protein equivalent of casein protein isolate dissolved in \~250 mL of water
30 g protein equivalent of whey protein isolate dissolved in \~250 mL of water
30 g protein equivalent of soy protein isolate dissolved in \~250 mL of water
30 g carbohydrate equivalent of maltodextrin powder dissolved in \~250 mL of water
Changes in metabolites found in plasma (plasma metabolomics)
Untargeted metabolomics profiling of plasma will be conducted using ultra performance liquid chromatography-quadrupole time of flight-mass spectrometry (UPLC-QTOF-MS)
Time frame: Baseline, 15, 30, 60, 90, 120, 180, 240, 300 minutes
Changes in metabolites found in urine (urinary metabolomics)
Untargeted metabolomics profiling of urine will be conducted using ultra performance liquid chromatography-quadrupole time of flight-mass spectrometry (UPLC-QTOF-MS)
Time frame: Baseline, 60, 120, 180, 240, 300 minutes
Cognitive Function
The Computerised Mental Performance Assessment System (COMPASS) will be administered via a laptop. The COMPASS tests consist of 5 consecutive tests, including Digit Vigilance, Stroop, Rapid Visual Information Processing, Computerised Corsi Blocks Lite, Alphabetic Working Memory and Serial Subtraction, where the correctness and reaction time will be recorded. The test is expected to take around 15 minutes.
Time frame: Baseline, 15, 60, 120, 180, 240, 300 minutes
Mood
The Profile of Mood States (POMS) questionnaire will be administered, which contains various words that describe feelings. Participants will indicate, for each word, how they currently feel on scale of 0 (Not at all) to 4 (Extremely).
Time frame: Baseline, 15, 60, 120, 180, 240, 300 minutes
Plan to share: No — Electronic copies of the data with identifiable participant information will be kept on NUS OneDrive with access limited only Dr Kim Jung Eun and her research staff. All data will be de-identified prior to statistical analyses.
This study is active, not recruiting, as verified in Apr 2025. You cannot join it, but the record below documents what was studied.
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National University of Singapore