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CompletedNCT06821204Updated Apr 24, 2025

Detection of Pathogenic Bacteria in Make-up Tools

An interventional study of Health education group and control group in Health Education, sponsored by Ufuk University. Completed at 1 site in Turkey. Open to female participants aged 18 Years to 30 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2025-04-24.

Sponsored by Ufuk University · Not applicable, Interventional, and Supportive care

Phase
Not applicable
Study type
Interventional
Enrollment
64
Allocation
Randomized
Ages
18 Years to 30 Years
Sex
Female
01

Study summary

In addition to collecting product residues, dirt and oil, makeup products can damage the skin and cause infection as they are a breeding ground for bacteria. Studies investigating the microbiological contamination of makeup applicators have shown that makeup sponges and brushes are a means of transmitting pathogens. Other studies have determined that positive isolates are detected in almost every device taken from makeup tools. In order to draw attention to the source of infection that is not widely known by the public and to instill correct hygiene habits, this study aims to detect pathogenic bacteria in makeup tools used by university students and to evaluate the effectiveness of the hygiene training provided.

Read the detailed description

Makeup does a good job of improving the appearance of women. However, many women do not know that makeup can be a health hazard because it contains bacteria and can spread infection. In addition to collecting product residue, dirt, and oil, makeup products can damage the skin and cause infection because they are a breeding ground for bacteria. Cosmetic contamination leads to various types of infections, ranging from mild to serious.

Studies investigating the microbiological contamination of used makeup applicators have shown that makeup sponges and brushes are a vehicle for pathogen transmission. Other studies have determined that Staphylococcus aureus grows as the dominant bacteria in makeup tools and that positive isolates are detected in almost every device taken. The American Academy of Dermatology recommends washing your makeup brushes every 7 to 10 days to protect your skin and kill any harmful bacteria left on your makeup brushes.

Infectious diseases are still a major public health problem, especially in developing countries. Therefore, it is important for people to adopt proper hygiene habits, especially hand washing, to prevent infection. In order to draw attention to a source of infection that is not widely known by the public and to instill correct hygiene habits, this study aims to detect pathogenic bacteria in make-up tools used by university students and to evaluate the effectiveness of the hygiene education provided.

02

Conditions studied

  • Health Education

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Keywords

  • Makeup Tools
  • Pathogenic Bacteria
  • Hygiene Education
03

In context

Health Education

481 studies on the registry are indexed under Health Education; 110 are open to participants now.

This study's enrollment of 64 is below the median of 132 across 450 interventional studies indexed under Health Education.

Browse Health Education studies →

Lead sponsor

Ufuk University is the lead sponsor of 41 studies on the registry; 3 are open to participants now.

Counted across the registry records on this site, refreshed daily.

04

Who can participate

Ages eligible
18 Years to 30 Years
Sexes eligible
Female
Accepts healthy volunteers
Yes

Go through the criteria one by one — yes, no or not sure — and take the list to your doctor or the study team. Kept in this browser only.

Inclusion criteria

  • Being registered at Ufuk University School of Nursing,
  • Being over 18 and under 30
  • Being female,
  • Having make-up tools,
  • Being volunteer to participate in the research.

Exclusion criteria

Exclusion Criteria:

  • Being male,
  • Not having make-up tools,
  • Giving up participating in the research,
  • Under 18 years old, over 30 years old
05

Study design

Phase
Not applicable
Primary purpose
Supportive care
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Double (Participant, Outcomes assessor)
Enrollment
64 participants (actual)

Study arms

  • Active comparator
    Healt Education Group

    Participants will be given one session (40 minutes) of training on the definition and importance of hygiene, the rules and importance of hand washing, the importance of cleaning make-up materials, which method, material and how often they should be cleaned, and the health risks that may occur if they are not cleaned.

    Other: Health education group

  • Sham comparator
    Control group

    No intervention will be applied to participants in this group.

    Other: control group

Interventions

  • OtherHealth education group

    Participants will be given one session (40 minutes) of training on the definition and importance of hygiene, the rules and importance of hand washing, the importance of cleaning make-up materials, which method, material and how often they should be cleaned, and the health risks that may occur if they are not cleaned.

  • Othercontrol group

    No intervention will be applied to participants in this group.

06

What researchers measure

Primary outcomes

  1. Hygiene Control Kit

    A kit designed to perform microbiological analysis in a practical way will be used to reveal the microorganism density and pathogenic microorganism presence in the make-up tools swab sample. The Hygiene Control Kit (Hytech Slide product) is in compliance with the ISO 18593:2004 Standard. The kit is a product that can perform analysis with medium contact from all surfaces for microbiological analysis. It is possible to take samples from all surfaces, personnel, equipment, solid and liquid. Thanks to its 90-degree bendable structure, the medium comes into contact with the surface even on difficult, inclined and narrow surfaces. E.coli and S.Aureus colonies will be counted after incubation.

    Time frame: two weeks

Secondary outcomes

  1. Individual information form

    The individual information form was developed based on relevant literature. The form includes 22 questions to determine the frequency and method of infection, hand washing, makeup use and cleaning of makeup tools, frequency of changing, and sharing with someone else.

    Time frame: two weeks

07

Study locations

1 site
  • Ufuk University
    Balgat, Ankara 06520, Turkey
08

References and documents

Individual participant data

Plan to share: No

No publications or documents are linked to this record.

09

Updates

Tracking since Sep 25, 2026
No changes since tracking began. The registry record was last updated on Apr 24, 2025, before this site started recording changes on Sep 25, 2026. Its history is on ClinicalTrials.gov ↗
10

Registry details

Key details

Study ID
NCT06821204
Lead sponsor
Ufuk University
Responsible party
Duygu Akçay (Assistant Professor, Ufuk University) — Principal investigator
First posted
Feb 11, 2025
Start date
Mar 20, 2025
Primary completion
Apr 18, 2025
Completion
Apr 18, 2025
Last update
Apr 24, 2025

Study contacts

Duygu Akçay, Asst. Prof.
principal investigator · Ufuk University
Aslı Genç, Asst. Prof.
principal investigator · Ufuk University
Nedim Sultan, Prof.Dr.
principal investigator · Ufuk University

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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This study is completed, as verified in Apr 2025. You cannot join it, but the record below documents what was studied.

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