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Enrolling by invitationNCT06515860NF1-TEDUpdated Aug 31, 2026

Neurofibromatosis Type 1 Tumor Early Detection Study

An observational study in Neurofibromatosis Type 1, Neurofibromatosis 1 and Plexiform Neurofibroma, sponsored by David Miller. Enrolling by invitation at 1 site in United States. Open to participants aged 18 Years and older. Per ClinicalTrials.gov, last updated 2026-08-31.

Sponsored by David Miller · Observational

Study type
Observational
Model
Cohort
Time perspective
Prospective
Enrollment
1,000
Ages
18 Years and older
Sex
All
01

Study summary

The goal of this observational study is to determine if a liquid biopsy (i.e. blood test) is an effective clinical tool for monitoring the development of malignant peripheral nerve sheath tumor (MPNST) among adults (18 years and older) with Neurofibromatosis Type 1 (NF1), compared to the current standard of care. The main questions it aims to answer are:

How effective is liquid biopsy compared to the current standard of care (clinical surveillance and imaging) for early detection of MPNST development among people with NF1? Can liquid biopsy offer a cost-effective method for early detection of MPNST in people with NF1? Also, can liquid biopsy provide earlier detection that potentially leads to better outcomes? Also, can offering liquid biopsy improve access to care for people experiencing barriers to access (such as minority populations or people in rural areas)?

At baseline, participants will be asked to:

  • Complete surveys to provide their demographic and NF1-related health information.
  • Report whether or not they are experiencing MPNST-related symptoms.
  • Provide blood samples (15 mL blood total between three tubes, which is approximately one tablespoon).

Every six months during the five-year follow-up period, participants will be asked to:

  • Complete additional surveys to report whether or not they are experiencing MPNST-related symptoms and/or if they have been diagnosed with a new MPNST.
  • Provide an additional blood sample (10 mL blood total in one tube).

If diagnosed with an MPNST by their healthcare provider during the follow-up period, participants will be asked to:

  • Complete an additional survey regarding their diagnosis and symptoms.
  • Provide an additional blood sample (10 mL blood in one tube).
  • In parallel, the study team will request a sample of tumor tissue from the care provider, if available.
02

Conditions studied

  • Neurofibromatosis Type 1
  • Neurofibromatosis 1
  • Plexiform Neurofibroma
  • Plexiform Neurofibromas
  • Malignant Peripheral Nerve Sheath Tumor
  • Malignant Peripheral Nerve Sheath Tumors
  • Atypical Neurofibroma

Keywords

  • Liquid biopsy
  • Cancer surveillance
  • Tumor early detection
03

Who can participate

Ages eligible
18 Years and older
Sexes eligible
All
Accepts healthy volunteers
No
Sampling method
Non-probability sample

Study population

The study population will be adults with NF1 who live in the United States.

Inclusion criteria

  • 18 years and older (adults only)
  • Neurofibromatosis Type 1 (NF1) diagnosis (2021 Revised Diagnostic Criteria, PMID: 34012067)
  • History of plexiform neurofibroma (PN)
  • Able to read and understand English or Spanish
  • Live in the USA

Exclusion criteria

Exclusion Criteria:

  • Are children (younger than 18 years old)
  • Do not have NF1
  • Have no evidence of PN
  • Are not able to read and understand English or Spanish
04

Study design

Observational model
Cohort
Time perspective
Prospective
Enrollment
1,000 participants (estimated)
Patient registry
No
Biospecimen retention
Samples with dna

Interventions

  • Diagnostic testLiquid biopsy for MPNST development

    Participants will be asked to complete a blood sample (liquid biopsy) at baseline (15 mL, approximately 1 tablespoon) and every 6 months (10 mL) for the five-year follow-up period.

05

What researchers measure

Primary outcomes

  1. Timing of MPNST detection by liquid biopsy

    Comparison will be made between date of clinical diagnosis versus the date when the tumor could be detected in a liquid biopsy sample.

    Time frame: From enrollment to the end of the five-year follow-up period

Secondary outcomes

  1. Number of participants with new MPNST

    New MPNST diagnosed by healthcare provider (Yes/No). The number of participants who develop an MPNST will be compared to the overall size of the cohort.

    Time frame: From enrollment to the end of the five-year follow-up period

06

Study locations

1 site
  • Boston Children's Hospital
    Boston, Massachusetts 02115, United States
07

References and documents

Publications

  • Miettinen MM, Antonescu CR, Fletcher CDM, Kim A, Lazar AJ, Quezado MM, Reilly KM, Stemmer-Rachamimov A, Stewart DR, Viskochil D, Widemann B, Perry A. Histopathologic evaluation of atypical neurofibromatous tumors and their transformation into malignant peripheral nerve sheath tumor in patients with neurofibromatosis 1-a consensus overview. Hum Pathol. 2017 Sep;67:1-10. doi: 10.1016/j.humpath.2017.05.010. Epub 2017 May 24. PubMed 28551330 ↗
  • Klein EA, Richards D, Cohn A, Tummala M, Lapham R, Cosgrove D, Chung G, Clement J, Gao J, Hunkapiller N, Jamshidi A, Kurtzman KN, Seiden MV, Swanton C, Liu MC. Clinical validation of a targeted methylation-based multi-cancer early detection test using an independent validation set. Ann Oncol. 2021 Sep;32(9):1167-1177. doi: 10.1016/j.annonc.2021.05.806. Epub 2021 Jun 24. PubMed 34176681 ↗
  • Chaudhuri AA, Chabon JJ, Lovejoy AF, Newman AM, Stehr H, Azad TD, Khodadoust MS, Esfahani MS, Liu CL, Zhou L, Scherer F, Kurtz DM, Say C, Carter JN, Merriott DJ, Dudley JC, Binkley MS, Modlin L, Padda SK, Gensheimer MF, West RB, Shrager JB, Neal JW, Wakelee HA, Loo BW Jr, Alizadeh AA, Diehn M. Early Detection of Molecular Residual Disease in Localized Lung Cancer by Circulating Tumor DNA Profiling. Cancer Discov. 2017 Dec;7(12):1394-1403. doi: 10.1158/2159-8290.CD-17-0716. Epub 2017 Sep 24. PubMed 28899864 ↗
  • Pemov A, Li H, Presley W, Wallace MR, Miller DT. Genetics of human malignant peripheral nerve sheath tumors. Neurooncol Adv. 2019 Nov 28;2(Suppl 1):i50-i61. doi: 10.1093/noajnl/vdz049. eCollection 2020 Jul. PubMed 32642732 ↗
  • Cortes-Ciriano I, Steele CD, Piculell K, Al-Ibraheemi A, Eulo V, Bui MM, Chatzipli A, Dickson BC, Borcherding DC, Feber A, Galor A, Hart J, Jones KB, Jordan JT, Kim RH, Lindsay D, Miller C, Nishida Y, Proszek PZ, Serrano J, Sundby RT, Szymanski JJ, Ullrich NJ, Viskochil D, Wang X, Snuderl M, Park PJ, Flanagan AM, Hirbe AC, Pillay N, Miller DT; Genomics of MPNST (GeM) Consortium. Genomic Patterns of Malignant Peripheral Nerve Sheath Tumor (MPNST) Evolution Correlate with Clinical Outcome and Are Detectable in Cell-Free DNA. Cancer Discov. 2023 Mar 1;13(3):654-671. doi: 10.1158/2159-8290.CD-22-0786. PubMed 36598417 ↗
  • Mautner VF, Asuagbor FA, Dombi E, Funsterer C, Kluwe L, Wenzel R, Widemann BC, Friedman JM. Assessment of benign tumor burden by whole-body MRI in patients with neurofibromatosis 1. Neuro Oncol. 2008 Aug;10(4):593-8. doi: 10.1215/15228517-2008-011. Epub 2008 Jun 17. PubMed 18559970 ↗
  • Pemov A, Li H, Patidar R, Hansen NF, Sindiri S, Hartley SW, Wei JS, Elkahloun A, Chandrasekharappa SC; NISC Comparative Sequencing Program; Boland JF, Bass S; NCI DCEG Cancer Genomics Research Laboratory; Mullikin JC, Khan J, Widemann BC, Wallace MR, Stewart DR. The primacy of NF1 loss as the driver of tumorigenesis in neurofibromatosis type 1-associated plexiform neurofibromas. Oncogene. 2017 Jun 1;36(22):3168-3177. doi: 10.1038/onc.2016.464. Epub 2017 Jan 9. PubMed 28068329 ↗

Individual participant data

Plan to share: Undecided — Investigators conducting the analysis at the end of the follow-up period will need access to IPD, but the plan to share IPD is not yet written.

08

Registry details

Key details

Study ID
NCT06515860
Lead sponsor
David Miller
Responsible party
David Miller (Principal Investigator, Boston Children's Hospital) — Sponsor-investigator
First posted
Jul 23, 2024
Start date
Aug 7, 2024
Primary completion
Jul 2030 (estimated)
Completion
Jul 2030 (estimated)
Last update
Aug 31, 2026

Study contacts

David T Miller, MD, PhD
principal investigator · Boston Children's Hospital

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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