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RecruitingNCT06029348CHARMUpdated Sep 16, 2026

Cerebrovascular Health and Resilience in Midlife

An interventional study of Paced Auditory Serial Addition Task in Psychological Stress, Cardiovascular Diseases and Cerebrovascular Disorders, sponsored by University of Pittsburgh. Recruiting at 1 site in United States. Open to participants aged 40 Years to 59 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-09-16.

Sponsored by University of Pittsburgh · Not applicable, Interventional, and Basic science

Phase
Not applicable
Study type
Interventional
Enrollment
450
Allocation
Not applicable
Ages
40 Years to 59 Years
Sex
All
01

Study summary

This study examines cardiovascular responses to a brief psychological challenge under laboratory conditions among adult volunteers. The study volunteers are asked to complete two study sessions. In one session, the volunteers are asked to provide demographic and health-related information via questionnaires and an interview. In that session, volunteers would then be asked to undergo evaluations of their body composition, blood pressure, heart rate, and other signs of cardiovascular function and health. Lastly, volunteers would be asked to take part in a brief and challenging psychological task after a period of rest while cardiovascular activity is measured. In the other session, volunteers are asked to undergo magnetic resonance imaging and to complete neuropsychological tests of memory, attention, and processing speed. In addition to these two sessions, volunteers are asked to complete online questionnaires. The study is designed to examine associations between transient changes in cardiovascular activity induced by the psychological task, measures of overall cardiovascular health, and measures of cerebrovascular health measured by magnetic resonance imaging. Study results are expected to provide new information about the relationships between cardiovascular activity changes to psychological challenges and cardiovascular and cerebrovascular health in adults.

Read the detailed description

This cross-sectional study examines the extent to which cardiovascular responses to the acute experience of psychological stress relate to signs of cerebrovascular health detected on magnetic resonance imaging. Study volunteers will include midlife adults who do not report having a history of clinical cardiovascular disease or dementia. Study volunteers will be asked to complete protocols to assess: behavioral, social, and biological correlates of cardiovascular and cerebrovascular health; arterial stiffness, endothelial function, and beat-to-beat blood pressure; cardiovascular changes to acute psychological stress; signs of cerebrovascular health; and, neuropsychological test performance. Cardiovascular responses to acute psychological stress will be examined in relation to indicators of brain function and structure, as well as indicators of cardiac and vascular function. Study results may help to increase knowledge about stress-related factors and cardiovascular sources of brain health.

02

Conditions studied

  • Psychological Stress
  • Cardiovascular Diseases
  • Cerebrovascular Disorders
03

Who can participate

Ages eligible
40 Years to 59 Years
Sexes eligible
All
Accepts healthy volunteers
Yes

Inclusion criteria

  • Able to attend 2 study visits at the University of Pittsburgh campus in Oakland (Pennsylvania)
  • Able to read and speak English

Exclusion criteria

Exclusion Criteria:

  • Uncontrolled hypertension (systolic blood pressure >160 or diastolic blood pressure >100 mmHg)
  • Current use of anticonvulsant, anti-Parkinson, neuroleptic, or antipsychotic medications
  • Current use of glucocorticoids (e.g., oral prednisone) or ephedrine
  • Current chronic conditions of the lungs, liver and kidney, as well as cancer (chemotherapy and radiation) treatment in the past 12 months (allowances are made for non-melanoma skin cancer treatment, controlled asthma, and allergy inhalers)
  • Self-reported major neurological disorders or brain injuries (e.g., multiple sclerosis, cerebral palsy, major head injury)
  • Self-reported psychotic illnesses (mania, bipolar disorder, schizophrenia)
  • Current pregnancy or childbirth within the past 6 months
  • Suspected mild cognitive impairment or dementia
  • Self reported sleep apnea
  • Self-reported history of myocardial infarction, stroke, or revascularization procedures, as well as treatment for cardiac arrhythmias
  • Self-reported lack of comfort to undergo MRI testing
  • Presence of implants or objects that are unsafe for MRI (e.g., tattooed eyeliner), as well as any other contraindications that would prevent MRI testing
  • Self-reported lack of comfort to undergo the laboratory math task
04

Study design

Phase
Not applicable
Primary purpose
Basic science
Allocation
Not applicable
Intervention model
Single group
Masking
None (open label)
Enrollment
450 participants (estimated)

Study arms

  • Experimental
    Paced Auditory Serial Addition Task

    For the Paced Auditory Serial Addition Task, volunteers are presented with single digit numbers (1-9), and are instructed to add any given number to the previously presented number, and call out the answer. Volunteers are told that their performance is being monitored by research assistants. Volunteers are provided with a signaling device that they can use to discontinue testing. Lastly, participants will be asked to complete brief questionnaires to assess their levels of experienced threat, challenge, valence, arousal, and sense of control.

    Behavioral: Paced Auditory Serial Addition Task

Interventions

  • BehavioralPaced Auditory Serial Addition Task

    Study volunteers will be asked to complete a 4-minute Paced Auditory Serial Addition Task, which is a standardized task that is widely used to study cardiovascular responses to acute psychological challenges (or stressors) in a laboratory setting.

05

What researchers measure

Primary outcomes

  1. Change in systolic blood pressure (SBP) from baseline to task

    The mean SBP from the baseline period will be subtracted from the mean of the task period.

    Time frame: Mean SBPs for the computation of change scores will be computed from the average of the 5 min baseline period and average of the 4 min task period

  2. White matter hyperintensity burden

    White matter hyperintensity burden will be computed as the total sum of the voxels segmented as hyperintensities multiplied by voxel dimensions to yield a total score (units of mm-cubed).

    Time frame: Derived from one acquisition sequence within in a 50 min multimodal MRI protocol

  3. Hippocampal volume

    Total bilateral hippocampal volume will be computed as the total sum of voxels automatically segmented.

    Time frame: Derived from one acquisition sequence within a 50 min multimodal MRI protocol

  4. Perivascular space volume

    Total perivascular space volume will be computed from segmented brain blood vessel-tissue interfaces.

    Time frame: Derived from one acquisition sequence in a 50 min multimodal MRI protocol

  5. Pulse wave velocity

    Pulse wave velocity in m/s will be assessed by dual impedance cardiography as a non-invasive indicator of arterial stiffness. This measure will be derived from the 5 minute baseline period.

    Time frame: 5 minutes

  6. Spontaneous baroreflex sensitivity

    Spontaneous baroreflex sensitivity in ms/mmHg will be computed by the sequence method applied to beat-to-beat blood pressure and electrocardiogram recordings.

    Time frame: 5 minutes

  7. Endothelial function

    Endothelial-dependent vasodilation will be measured non-invasively in a reactive hyperemia protocol using digital thermal monitoring.

    Time frame: 15 minutes

06

Study locations

1 of 1 sites recruiting
  • University of Pittsburgh
    Pittsburgh, Pennsylvania 15213, United States
    • Sara Boyko, BS · Contact
    Recruiting
07

References and documents

Individual participant data

Plan to share: Yes — De-identified individual participant data that comprise primary and secondary study outcomes will be made available with data dictionaries via the University of Pittsburgh's repository, D-Scholarship@Pitt. This repository provides indefinite storage and accessibility. Along with de-identified data, other shared resources will include a description of data collection methods and study code book; a description of the data files; and descriptions of data use conditions. Statistical scripts will be deposited as appropriate following publication of manuscripts bearing on the aims of the study. All publications will include information on the location of study data and accessibility. Requests by researchers for other de-identified data will be facilitated by completion of a Data Use Agreement, as well as provision of a methodologically sound proposal and approval from their institution.

Supporting information: Study protocol

No publications or documents are linked to this record.

08

Registry details

Key details

Study ID
NCT06029348
Lead sponsor
University of Pittsburgh
Collaborators
National Heart, Lung, and Blood Institute (NHLBI)
Responsible party
Peter Gianaros (Professor, University of Pittsburgh) — Principal investigator
First posted
Sep 8, 2023
Start date
Oct 17, 2023
Primary completion
Jun 30, 2029 (estimated)
Completion
Jun 30, 2029 (estimated)
Last update
Sep 16, 2026

Study contacts

Peter J Gianaros, PhD
Contact
bnl@pitt.edu
412-624-4500
Sara Boyko, BS
Contact
bnl@pitt.edu
412-648-9047
Peter J Gianaros, PhD
principal investigator · University of Pittsburgh

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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