An interventional study of Extracorporeal shockwave device and No ESWT in Osteosarcoma, sponsored by Krishnadevaraya College of Dental Sciences & Hospital. Completed at 1 site in India. Per ClinicalTrials.gov, last updated 2023-04-06.
Sponsored by Krishnadevaraya College of Dental Sciences & Hospital · Not applicable, Interventional, and Treatment
The idea of treating different deformities or diseases in the maxillofacial region with Extracorporeal Shockwave Therapy (ESWT) has recently become popular. It has been widely used in medical practice for the management of urolithiasis, cholelithiasis and in haead and neck region for sialolithiasis. The present study 'Extracorporeal Shock Wave Therapy on Osteoblast-Like Cells In-vitro study' will be done to explore and evaluate the effect of shockwaves. Further, Osteoblast-like cells will be assessed for Cell - Cell interaction and Cell Viability. It will be assessed using a scratch assay, Mtt assay and ATP analysis.
Introduction:
Extracorporeal Shockwave Therapy (ESWT) has recently become popular. Shockwave has been widely used in medical practice for the management of urolithiasis, cholelithiasis and in haead and neck region for sialolithiasis.
Aim:
This study 'Extracorporeal Shock Wave Therapy on Osteoblast-Like Cells In-vitro study' will be done to explore and evaluate the effect of shockwaves. Further, Osteoblast-like cells will be assessed for Cell - Cell interaction and Cell Viability.
Methodology:
In this in-vitro study, 20 Osteoblast - like cells will be included. 10 samples to be considered for Control group and the remaining 10 samples considered as Test group. Extracorporeal shockwave will be applied to the Test samples. They will be further analysed using wound scratch assay which is used to assess Cell proliferative rate and scratch closure through live imaging. MTT assay and ATP assay used to determine Cell viability after exposure to shockwaves. The cell migration and cell viability after exposure to shockwaves will determine its effects on osteoblast-like cells.
437 studies on the registry are indexed under Osteosarcoma; 116 are open to participants now.
This study's enrollment of 20 is below the median of 42 across 325 interventional studies indexed under Osteosarcoma.
Browse Osteosarcoma studies →Krishnadevaraya College of Dental Sciences & Hospital is the lead sponsor of 51 studies on the registry; 13 are open to participants now.
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Exclusion Criteria:
The osteoblast like cells are treated with shockwaves
Device: Extracorporeal shockwave device
The osteoblast like cells are not exposed to shockwaves
Device: No ESWT
Extracorporeal shockwave device generates true shock waves which are characterized by a sudden rise in pressure followed by an exponential decay. This is different from the commercially available shockwave devices in which they use piezoelectric based mechanisms which generate high frequency waves.
Also known as: ESWT
the osteoblast like cells are not exposed to shockwaves
Also known as: shockwave
Scratch assay
cell migration
Time frame: 72hours
MTT Assay and ATP analysis
Cell viability
Time frame: 74hrs
This study is completed, as verified in Mar 2023. You cannot join it, but the record below documents what was studied.
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Krishnadevaraya College of Dental Sciences & Hospital