CClinicalTrials.gg
Active, not recruitingNCT05725018Updated Jun 27, 2025

A Phase 3b Study for the Treatment of Dystrophic Epidermolysis Bullosa (DEB) in New and Previously EB-101 Treated Patients

A Phase 3 interventional study of EB-101 Surgical application of RDEB wounds in Epidermolysis Bullosa, Recessive Dystrophic Epidermolysis Bullosa and RDEB, sponsored by Abeona Therapeutics, Inc. Active, not recruiting at 2 sites in United States. Open to participants aged 12 Months and older. Per ClinicalTrials.gov, last updated 2025-06-27.

Sponsored by Abeona Therapeutics, Inc · Phase 3, Interventional, and Treatment

Phase
Phase 3
Study type
Interventional
Enrollment
12
Allocation
Not applicable
Ages
12 Months and older
Sex
All
01

Study summary

To evaluate and further characterize the safety of EB-101 (LZRSE-Col7A1 gene-corrected keratinocyte sheets with type VII collagen [C7] expression) for the treatment of large, chronic DEB wounds in new and previously EB-101 treated patients 12 months and older.

Read the detailed description

A multicenter, open-label, single-arm Phase 3b safety study of one-time surgical application of up to 12 autologous, LZRSE-Col7A1 gene-corrected keratinocyte sheets with C7 expression (EB-101) for the treatment of large, chronic DEB wounds in each of approximately 10-12 patients. Patients enrolled will also contribute biopsies to support manufacturing requirements. All patients will be followed through 24 weeks post-treatment.

Patients will be evaluated at their Screening Visit (D -60 to D -25), a phone call approximately 14 days after screening, and at Baseline (Day -1) prior to treatment on Day 0. Patients will remain in the hospital, or other suitable inpatient medical facility that will allow appropriate immobilization of treated wounds, as determined by the Investigator, for observation for up to 7 days (±2 days) following treatment. They will be evaluated by phone on Day 14; by telehealth visits on Weeks 4, 8, and 18; and by clinic visits on Weeks 12 and 24.

02

Conditions studied

  • Epidermolysis Bullosa
  • Recessive Dystrophic Epidermolysis Bullosa
  • RDEB

Keywords

  • Chronic wounds
03

Who can participate

Ages eligible
12 Months and older
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  1. Clinical diagnosis of DEB.
  2. Age 12 months and older.
  3. Willing and able to give consent/assent; if under the age of 18 years, guardian(s) is/are willing and able to give consent.
  4. (This inclusion criterion was deleted as of Amendment 1.)
  5. Confirmation of DEB diagnosis (either dominant [DDEB] or recessive [RDEB] forms) by genetic testing.
  6. Able to undergo adequate anesthesia during EB-101 treatment.
  7. All women of childbearing potential must have a negative urine pregnancy test and use a reliable birth control method throughout the duration of the study.
  8. On stable pain medication regimen for at least 30 days prior to Screening (and through Baseline).
  9. Must have at least one wound site that meets all of the following criteria:

    1. An area ≥20 cm2,
    2. Present for ≥6 months, and
    3. Stage 2 wound defined as an open skin wound with partial thickness loss of dermis that has not extended through the dermis into subcutaneous tissue.
  10. For patients with RDEB, they must have had Vyjuvek exposure by the time of screening or positive anti-C7 antibodies at baseline.
  11. Patients must be willing to discontinue Vyjuvek and Filsuvez use on EB-101 treated sites until completion of the Week 24 visit.

Exclusion criteria

Exclusion Criteria:

  1. Medical instability limiting ability to travel to the study site or undergo EB-101 treatment.
  2. The presence of medical illness expected to complicate participation and/or compromise the safety of this technique, such as active infection with human immunodeficiency virus (HIV), hepatitis B, or hepatitis C.
  3. (This exclusion criterion was deleted as of Amendment 3.)
  4. Evidence of systemic infection.
  5. Current evidence or a history of SCC in the area that will undergo EB-101 application.
  6. Active drug or alcohol addiction.
  7. Hypersensitivity to vancomycin or amikacin.
  8. Receipt of chemical or biological investigational therapy for the specific treatment of DEB in the 3 months prior to EB-101 application.
  9. Breast-feeding.
  10. Inability to properly follow protocol assessments and protect keratinocyte sheet sites as determined by the PI.
  11. Grade 3 clinical event or laboratory abnormality at Day 0. Abnormalities such as esophageal strictures, anemia, low albumin, and pain/itch are expected in severe DEB patients, and these abnormalities will not exclude a patient.
  12. Unwillingness or inability to provide 4 skin biopsies, or patient's keratinocytes cannot be manufactured for use in EB-101 application.
  13. Any other circumstance where the PI believes that the patient may not be appropriate for participation in the study.
04

Study design

Phase
Phase 3
Primary purpose
Treatment
Allocation
Not applicable
Intervention model
Single group
Masking
None (open label)
Enrollment
12 participants (estimated)

Study arms

  • Experimental
    EB-101 Surgical application of RDEB wounds

    New or Previously Treated RDEB Patients

    Biological: EB-101 Surgical application of RDEB wounds

Interventions

  • BiologicalEB-101 Surgical application of RDEB wounds

    EB-101 autologous RDEB keratinocytes isolated from skin biopsies and transduced with a recombinant retrovirus containing a full-length COL7A1 expression cassette for C7 Other Names: • LZRSE-Col7A1 Engineered Autologous Epidermal Sheets \[LEAES\]

    Also known as: pz-cel

05

What researchers measure

Primary outcomes

  1. Safety Endpoint (number of treatment-related and treatment-emergent adverse events (AEs) and serious adverse events (SAEs)

    The number of treatment-related and treatment-emergent adverse events (TEAEs), treatment-emergent wound adverse events (TEWAEs), and serious adverse events (SAEs), including systemic and wound-specific adverse events.

    Time frame: 6 months

  2. Safety Endpoint (number of patients and wounds that have an infection or any related adverse event.)

    The number of patients and wounds that have an infection or any related adverse event (AE).

    Time frame: 6 months

  3. Safety Endpoint (number of patients and wounds that result in hospitalization (serious adverse event )

    The number of patients and wounds that result in hospitalization (SAE).

    Time frame: 6 months

  4. Safety Endpoint (Incidence of squamous cell carcinoma)

    The incidence of squamous cell carcinoma (SCC).

    Time frame: 6 months

  5. Safety Endpoint RCR status

    Replication-competent retrovirus (RCR) status.

    Time frame: 6 months

Other outcomes

  1. Proportion of RDEB wounds with ≥50%, ≥75%, and complete

    Proportion of RDEB wounds with ≥50%, ≥75%, and complete (i.e., re epithelialization with no drainage or erosion and presence of only minor crusting) healing at Weeks 12 and 24, as determined by direct Investigator assessment.

    Time frame: 6 months

  2. Extent of pain reduction assessed by the Wong-Baker FACES scale

    Extent of pain reduction assessed by the Wong-Baker FACES scale (for patients ages ≥6 years old) at Weeks 8, 12, and 24.

    Time frame: 6 months

  3. Longitudinal change in scores of Worst Itch Numeric Rating Scale

    Longitudinal change in scores of Worst Itch Numeric Rating Scale (WI-NRS; for patients ages ≥6 years old) scores assessed at Weeks 8, 12, and 24

    Time frame: 6 months

  4. Longitudinal change of Zarit Burden Interview Short Form

    Longitudinal change of Zarit Burden Interview Short Form (ZBI-12) for caregiver scores related to wound care, assessed at Weeks 12 and 24 (for all ages).

    Time frame: 6 months

  5. Change in Quality of Life in Epidermolysis Bullosa

    Change in Quality of Life in Epidermolysis Bullosa (QOLEB) scores assessed at Week 24 compared with pretreatment Baseline scores (completed by patients ≥11 years old or with guidance for patients 8 to 10 years old). If patient is \<8 years old, QOLEB should be completed by a legal guardian.

    Time frame: 6 months

  6. Change in Caregiver Global Impression of Pain

    Change in Caregiver Global Impression of Pain (CrGI-Pain) scores assessed at Weeks 8, 12, and 24 compared with pretreatment Baseline (for all ages).

    Time frame: 6 months

  7. Comparison of how non-collagenous region 1 of the collagen VII molecule

    Comparison of how non-collagenous region 1 of the collagen VII molecule (NC1) status relates to wound healing and safety profile (if applicable).

    Time frame: 6 months

  8. Comparison of how circulating C7 antibody status relates to wound healing and safety profile

    Comparison of how circulating C7 antibody status relates to wound healing and safety profile (if applicable).

    Time frame: 6 months

06

Study locations

2 sites
  • Stanford University
    Redwood City, California 94063, United States
  • University of Massachusetts Medical School
    Worcester, Massachusetts 01605, United States
07

References and documents

Individual participant data

Plan to share: No

No publications or documents are linked to this record.

08

Registry details

Key details

Study ID
NCT05725018
Lead sponsor
Abeona Therapeutics, Inc
Responsible party
Sponsor
First posted
Feb 13, 2023
Start date
Apr 2, 2023
Primary completion
Sep 30, 2025 (estimated)
Completion
Sep 30, 2025 (estimated)
Last update
Jun 27, 2025

Study contacts

Angela Iheanacho, MS
study director · Abeona Therapeutics, Inc
Sarah Abdelwahab, MD
study director · Abeona Therapeutics, Inc

Oversight

Data monitoring committee
Yes
FDA-regulated drug
Yes
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

Not currently enrolling

This study is active, not recruiting, as verified in Jun 2025. You cannot join it, but the record below documents what was studied.

Follow this study

Get an email when the registry record changes — status, dates, results — or when someone posts here.

Sign in to follow

Discussion

Questions and observations about this study, from anyone following it. Not medical advice, and not a channel to the study team — their contact details are on the registry record.

Sign in to join the discussion. Reading takes no account; posting does. You choose a display name, and a pseudonym is the default.

Nothing here yet. If you are running this trial, taking part in it, or weighing whether to, this is the place to say so.

Start the discussion