Optimal Conditioning Regimen for Autologous Transplantation of Relapsing Remitting Multiple Sclerosis

1. Age 18-58 years old
2. MRI T2 hyperintense lesions with at least 1 lesion in two or more of the following locations: periventricular, cortical or juxtacortical, infratentorial, or 1 spinal lesion
3. Since diagnosis a new MRI T2 lesion or since diagnosis a gadolinium positive lesion and at least one T2 weighted lesion

4. RRMS with a history of:

   1. 2 or more "active flares" in the prior 12 months despite either copaxone or interferon; or
   2. 1 or more "active flares" in the prior year despite a 2nd or 3rd generation DMT; or
   3. Active secondary progressive MS (aSPMS) with 2 or more gadolinium enhancing lesions with at least 1 gadolinium enhancing lesion > 5 mm in longest dimension within the last 9 months

Exclusion Criteria:

1. CIS- clinically isolated lesion
2. isolated optic neuritis
3. Primary progressive MS b) Nonactive SPSM (defined as no new or enhancing lesions in last 12 months)
4. spasticity or clinical stiffness of leg(s) unless there is documented new MRI enhancement within the past 12 months.
5. hyperreflexia or clonus
6. other immune neurologic disease such as NMO, CIDP, Stiff person syndrome, myasthenia gravis
7. genetic neurologic diseases such as CMT or spinal cerebellar degeneration
8. another autoimmune diagnosis such as systemic lupus erythematosus, systemic sclerosis, Behcets, or crohn's disease, etc (with the exception of hypo or hyperthyroidism or history of ITP or AIHA that is in remission)
9. insulin dependent diabetes mellitus
10. sickle cell disease
11. thalassemia major
12. porphyria
13. a current or prior cancer / malignancy except for cutaneous basal cell carcinoma or carcinoma in situ (completely excised)

14. Hepatic:

    1. Liver function test (AST or ALT) > 2 x upper limit of normal or
    2. bilirubin > 2.0 mg /dl

15. Pulmonary:

    1. DLCO \< 60% of normal or;
    2. Asthma not easily corrected with bronchodilator therapy or;
    3. Pulmonary artery hypertension (pulmonary artery systolic pressure (PASP) > 40 mmHg on echocardiogram or by cardiac catheterization a mean pulmonary artery pressure (mPAP) > 25 mmHg; a cardiac catheterization for pulmonary artery pressures is only performed if clinically indicated)

16. Renal:

    1. creatinine > 2.0 mg/dl, or
    2. nephrotic syndrome

17. Cardiac:

    1. Acute myocardial infarction (AMI) within the last year, and if history of AMI not being approved by cardiology as low risk or not at increased risk for another AMI: or
    2. Persistent arrythmia not controlled with medication;
    3. Any patient requiring medication for an arrhythmia must be pre-approved by cardiology, or
    4. left ventricular ejection fraction \< 45%

18. Hematology

    1. Hereditary coagulopathy or currently receiving anticoagulation therapy
    2. platelets \< 100,000
    3. myelodysplastic syndrome

19. Infection:

    1. HIV,
    2. hepatitis B
    3. hepatitis C
    4. positive quantiferon gold (tuberculosis test) (may start HSCt once seen by ID and started on anti-tuberculous therapy, that will be continued throughout transplant, if asymptomatic),

    d) active infection at time of hospital admission (except UTI)

20. EDSS \< 2.0 at time of enrollment or insurance submission
21. Inability to comprehend or give or sign informed consent
22. Pregnancy (positive serum or urine HCG test) or breast feeding
23. Failure to comprehend infertility as a complication.
24. Failure to offer sperm or oocyte collection and storage
25. Before HSCT failure to be Free of alemtuzumab for 12 months
26. Before HSCT failure to be Free of natalizumab for 5 months
27. Before HSCT failure to be Free of rituximab or ocrelizumab for 5 months
28. Before HSCT failure to be Free of fingolimod for 3 months
29. Before HSCT failure to be Free of dimethyl fumarate (tecfidera) for 3 months
30. Before HSCT failure of teriflunomide to have plasma levels \< 0.02 mg/L after either oral cholestyramine or activated charcoal clearance.
31. Prior mitoxantrone
32. Prior cladribine