CClinicalTrials.gg
CompletedNCT04764565Updated Apr 28, 2021

Impact of Nuun Instant on Hydration Status in Active Men and Women

An interventional study of Nuun Instant and Water in Hydration, sponsored by University of Memphis. Completed at 1 site in United States. Open to participants aged 18 Years to 45 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2021-04-28.

Sponsored by University of Memphis · Not applicable, Interventional, and Basic science

Phase
Not applicable
Study type
Interventional
Enrollment
20
Allocation
Randomized
Ages
18 Years to 45 Years
Sex
All
01

Study summary

While Nuun Instant is commercially available and has received positive reviews from athletes, there have been no studies to date to evaluate the impact of this product on hydration status. Therefore, the purpose of this study is to determine the impact of Nuun Instant powder and new product under development called Nuun Electrolyte on hydration status in young and active men and women. We will follow a similar approach as used by Maughan and colleagues (2016) to measure the fluid balance (the difference between the amount of water consumed and passed as urine) and beverage hydration index (the relative amount of urine passed after consumption of a drink compared to water), while also measuring plasma volume using the method of Dill and Costill (1974).

Read the detailed description

Maintaining adequate hydration is essential to optimal health and athletic performance. When individuals exercise (in particular in a warm environment), they can lose excessive amounts of fluids through sweating, along with necessary electrolytes (e.g., sodium, potassium, chloride). With dehydration, individuals may feel sluggish and can experience impaired physical performance.

Many attempts have been made to improve and measure the hydration status of active individuals. This typically involves the ingestion of fluids leading up to activity (typically plain water), as well as the ingestion of fluids during the activity itself (water, along with a diluted carbohydrate/electrolyte beverage). This approach seems to work well; however, some debate remains over what the best fluid is to consume, in particular related to the macronutrient type and the specific electrolyte mix.

Related to the above, it is well-accepted that electrolyte replenishment is of importance, both during and following exercise-to aid in rehydration for subsequent bouts. Electrolytes (sodium in particular) have been used for decades to aid athlete hydration and this has led to the development of various sport drinks-which also include small amounts of carbohydrate (e.g., Gatorade, Powerade). However, one problem with moderate to high carbohydrate ingestion is that some individuals experience gastrointestinal (GI) upset following carbohydrate ingestion before and during an event, despite very good physical performance outcomes. Due to this GI upset, some individuals (in particular recreationally active individuals who are not competing at high levels) rely on water alone or a very low concentrated carbohydrate beverage, and seek a method to ingest the lost electrolytes as well.

The solution to the above issues for many athletes is the use of powder "stick packs" that can be carried in bags and pockets, and simply dumped into a water bottle when needed, shaken, and consumed. They are easy to transport and provide the needed carbohydrate, as well as electrolytes to replace those that are lost through intense and/or long duration exercise.

We recently completed a study of Nuun electrolyte tablets, which provide 2 grams of carbohydrate per serving and a combination of electrolytes, provided at a relatively low percentage of the Daily Value (DV). The DV tells us how much a particular nutrient in a serving of a food product contributes to a daily diet, based on a standard 2000 calorie diet. Nuun tablets contain: calcium (13mg, 1%DV), sodium (300mg, 13%DV), potassium (150mg, 3%DV), magnesium (25mg, 6%DV), and chloride (40mg, 2%DV). Our results indicated improved hydration (based on urine output and the calculated hydration index) when subjects consumed the Nuun tablets mixed into 1 liter of water, as compared to water alone.

Nuun Instant is a new product that is similar to the Nuun tablets, but is formulated for those who may experience a significant electrolyte loss and is higher in carbohydrate as well. Specifically, the Nuun Instant product contains (per 11 gram serving) the following: 7 grams of carbohydrate, calcium (20mg, 2%DV), sodium (520mg, 23%DV), potassium (385mg, 8%DV), magnesium (8mg, 2%DV), chloride (250mg, 10%DV), and 100% of vitamin C (90mg) and B12 (2.4 mcg).

Nuun Electrolytes is a new product in development that is similar to the Nuun tablets, but with an adjusted formula. Nuun Electrolytes consist of Citric Acid, Dextrose, Sodium Bicarbonate, Potassium Bicarbonate, Sodium Carbonate, Natural Flavors, Potassium Chloride, Malic Acid, Magnesium Carbonate, Calcium Carbonate, Stevia Leaf Extract, Beet Powder Color, Safflower Oil. Nuun electrolytes contains (per tablet) the following: 3 grams of carbohydrate, calcium (15 mg, 1%DV), sodium (200 mg, 9%DV), potassium (125mg, 3%DV), magnesium (15mg, 4%DV), and chloride (75mg, 3%DV).

While Nuun Instant is commercially available and has received positive reviews from athletes, there have been no studies to date to evaluate the impact of this product or Nuun Electrolyte on hydration status. Therefore, the purpose of this study is to determine the impact of Nuun Instant powder and Nuun Electrolyte tablets on hydration status in young and active men and women. We will follow a similar approach as used by Maughan and colleagues (2016) to measure the fluid balance (the difference between the amount of water consumed and passed as urine) and beverage hydration index (the relative amount of urine passed after consumption of a drink compared to water), while also measuring plasma volume using the method of Dill and Costill (1974).

02

Conditions studied

  • Hydration

Keywords

  • electrolyte
  • sport drink
  • beverage hydration index
03

In context

Lead sponsor

University of Memphis is the lead sponsor of 44 studies on the registry; 2 are open to participants now.

Counted across the registry records on this site, refreshed daily.

04

Who can participate

Ages eligible
18 Years to 45 Years
Sexes eligible
All
Accepts healthy volunteers
Yes

Inclusion criteria

  • body mass index (BMI) between 18-29.9 kg/m2
  • no consumption of alcohol-containing beverages within 48 hours of testing
  • no consumption of caffeine-containing beverages within 48 hours of testing
  • no strenuous exercise within 48 hours of testing
  • regularly consumes 2 liters of water daily
  • engaged in structured exercise 3 or more hours per week for the past 6 months or longer

Exclusion criteria

Exclusion Criteria:

  • tobacco user
  • active infection or illness
  • pregnant
05

Study design

Phase
Not applicable
Primary purpose
Basic science
Allocation
Randomized
Intervention model
Crossover assignment
Masking
Single (Participant)
Enrollment
20 participants (actual)

Study arms

  • Experimental
    Nuun Instant

    2 servings of Nuun instant in 1 liter water

    Dietary Supplement: Nuun Instant · Other: Water

  • Placebo comparator
    Control

    1 liter of water

    Other: Water

  • Experimental
    Nuun Electrolyte

    2.1 servings of Nuun Electrolyte in 1 liter water

    Other: Water · Dietary Supplement: Nuun Electrolyte

Interventions

  • Dietary supplementNuun Instant

    Contains (per 11 gram serving) the following: 7 grams of carbohydrate, calcium (20mg, 2%DV), sodium (520mg, 23%DV), potassium (385mg, 8%DV), magnesium (8mg, 2%DV), chloride (250mg, 10%DV), and 100% of vitamin C (90mg) and B12 (2.4 mcg).

  • OtherWater

    water

  • Dietary supplementNuun Electrolyte

    Citric Acid, Dextrose, Sodium Bicarbonate, Potassium Bicarbonate, Sodium Carbonate, Natural Flavors, Potassium Chloride, Malic Acid, Magnesium Carbonate, Calcium Carbonate, Stevia Leaf Extract, Beet Powder Color, Safflower Oil. Nuun electrolytes contains (per tablet) the following: 3 grams of carbohydrate, calcium (15 mg, 1%DV), sodium (200 mg, 9%DV), potassium (125mg, 3%DV), magnesium (15mg, 4%DV), and chloride (75mg, 3%DV).

06

What researchers measure

Primary outcomes

  1. Body weight

    Body weight will be measured in underwear and papergown on digital scale.

    Time frame: baseline

  2. Body weight

    Body weight will be measured in underwear and papergown on digital scale.

    Time frame: 4 hours after intervention

  3. Urine Volume

    Volume of urine output will be measured.

    Time frame: Immediately following intervention

  4. Urine Volume

    Volume of urine output will be measured.

    Time frame: 1 hour after intervention

  5. Urine Volume

    Volume of urine output will be measured.

    Time frame: 2 hours after intervention

  6. Urine Volume

    Volume of urine output will be measured.

    Time frame: 3 hours after intervention

  7. Urine Volume

    Volume of urine output will be measured.

    Time frame: 4 hours after intervention

  8. Urine Mass

    Urine output will be measured using an analytical scale.

    Time frame: immediately after intervention

  9. Urine Mass

    Urine output will be measured using an analytical scale.

    Time frame: 1 hour after intervention

  10. Urine Mass

    Urine output will be measured using an analytical scale.

    Time frame: 2 hours after intervention

  11. Urine Mass

    Urine output will be measured using an analytical scale.

    Time frame: 3 hours after intervention

  12. Urine Mass

    Urine output will be measured using an analytical scale.

    Time frame: 4 hours after intervention

  13. Blood Pressure

    Blood Pressure will be measured.

    Time frame: baseline

  14. Blood Pressure

    Blood Pressure will be measured.

    Time frame: 1 hour after intervention

  15. Blood Pressure

    Blood Pressure will be measured.

    Time frame: 2 hours after intervention

  16. Blood Pressure

    Blood Pressure will be measured.

    Time frame: 3 hours after intervention

  17. Blood Pressure

    Blood Pressure will be measured.

    Time frame: 4 hours after intervention

  18. Heart Rate

    Heart rate will be measured.

    Time frame: immediately after intervention

  19. Heart Rate

    Heart rate will be measured.

    Time frame: 1 hour after intervention

  20. Heart Rate

    Heart rate will be measured.

    Time frame: 2 hours after intervention

  21. Heart Rate

    Heart rate will be measured.

    Time frame: 3 hours after intervention

  22. Heart Rate

    Heart rate will be measured.

    Time frame: 4 hours after intervention

  23. Hematocrit

    Hematocrit will be measured from blood.

    Time frame: baseline

  24. Hematocrit

    Hematocrit will be measured from blood.

    Time frame: 4 hours after intervention

  25. Hemoglobin

    Hemoglobin will be measured from blood.

    Time frame: baseline

  26. Hemoglobin

    Hemoglobin will be measured from blood.

    Time frame: 4 hours after intervention

07

Study locations

1 site
  • Center for Nutraceutical and Dietary Supplement Reseach
    Memphis, Tennessee 38152, United States
08

References and documents

Individual participant data

Plan to share: No

No publications or documents are linked to this record.

09

Updates

Tracking since Sep 25, 2026
No changes since tracking began. The registry record was last updated on Apr 28, 2021, before this site started recording changes on Sep 25, 2026. Its history is on ClinicalTrials.gov ↗
10

Registry details

Key details

Study ID
NCT04764565
Lead sponsor
University of Memphis
Collaborators
Nuun & Company
Responsible party
Richard Bloomer (Dean of the College of Health Sciences, University of Memphis) — Principal investigator
First posted
Feb 21, 2021
Start date
Feb 23, 2021
Primary completion
Apr 6, 2021
Completion
Apr 6, 2021
Last update
Apr 28, 2021

Study contacts

Richard Bloomer, PhD
principal investigator · University of Memphis

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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