CClinicalTrials.gg
CompletedNCT04549909BPLUpdated Sep 23, 2020

Biochemical Pregnancy Loss. A Multicenter Retrospective Study

An observational study in Pregnancy Loss, Early, sponsored by IVI Vigo. Completed at 1 site in Spain. Open to female participants aged 18 Years to 44 Years. Per ClinicalTrials.gov, last updated 2020-09-23.

Sponsored by IVI Vigo · Observational

Study type
Observational
Model
Cohort
Time perspective
Retrospective
Enrollment
20,000
Ages
18 Years to 44 Years
Sex
Female
01

Study summary

Biochemical pregnancy loss (BPL) is a very frequent issue in human reproduction. After the implantation of the embryo, hCG disappears very soon from the maternal bloodstream and no evidence of a clinical pregnancy is seen. Different studies showed that factors such as age, oocyte and embryo quality, and endometrium receptivity may have something to do with the occurrence of biochemical pregnancy loss post assisted reproduction treatment.

The main aim of this study is to evaluate the incidence of biochemical pregnancy loss (BPL) in three different cohort populations; patients undergoing frozen embryo transfer (FET) from own oocytes after preimplantation genetic testing for aneuploidy (PGT-A), patients undergoing FET from own and donated oocytes and with endometrial receptivity array (ERA), and patients undergoing FET from own or donated oocytes (without PGTA or ERA test).

We will analyse the incidence of BPL in these populations and try to determine the role of the euploid status embryo in the first group, the endometrium in the second group and the third one as control group. We are waiting to find the value of both players in the origin of BPL.

Read the detailed description

Human embryo implantation is a poorly understood process. Once the embryo implants in the endometrium, it starts to secrete hCG that can be measured in the maternal blood as early as 9 days after implantation. Only a minimal number of pregnancies get to newborn, and the majority are lost before reach the first trimester (Larsen et al., 2013).

We are looking for the role of the embryo after controlling its chromosomal ploidy, and the endometrium after controlling its transcriptomic expression. We will also use a no exposed group to the controlled euploid embryo factor neither endometrial factor that is the oocyte donation group. This analysis expects to provide more information about the key role of embryo or endometrium in BPL.

02

Conditions studied

  • Pregnancy Loss, Early

Keywords

  • pregnancy
  • abortion
  • bioquemical
03

Who can participate

Ages eligible
18 Years to 44 Years
Sexes eligible
Female
Accepts healthy volunteers
No
Sampling method
Non-probability sample

Study population

Infertile patients who come to participating centres for assisted reproduction treatments. Patients will be divided into 4 groups according to the treatment carried out: PGT-A, ERA, FET from own oocytes (CO) or FET from donated oocytes (CD).

Inclusion criteria

Patients with the following selection criteria:

  • IVF/ICSI patients aged between 18 and 44
  • BMI 18-30 kg/m2
  • Frozen embryo transfer from own oocytes after PGT-A
  • Frozen embryo transfer with ERA test (from own or donated oocytes)
  • Frozen embryo transfer (from own or donated oocytes)
  • Single embryo transfer (SET) in all cycles
  • Patients without uterine malformations
  • Patients without recurrent miscarriage (≥ 3)
  • Patients with adequate endometrial thickness (> 7mm)
  • Patients without thyroid autoimmunity
  • Patients without thrombophilia
  • Exclude cycles with exclusively PGT-M
  • Exclude FET in ovarian stimulated cycles

Exclusion criteria

Exclusion Criteria:

Exclude cycles with exclusively PGT-M Exclude FET in ovarian stimulated cycles

-

04

Study design

Observational model
Cohort
Time perspective
Retrospective
Enrollment
20,000 participants (actual)
Patient registry
No

Groups and cohorts

  • preimplantation genetic testing for aneuploidy (PGT-A) Group

    Patients who have undergone preimplantation genetic testing for aneuploidy (PGT-A) (transfer of own frozen embryo)

    Other: collect retrospectively data

  • endometrial receptivity array (ERA) Group

    Patients who have undergone frozen embryo transfer (FET) with endometrial receptivity array (ERA) test (embryos from own or donated oocytes)

    Other: collect retrospectively data

  • CONTROL OWN (CO) Group

    Control group of FET from own oocytes (without ERA or PGT-A)

    Other: collect retrospectively data

  • CONTROL DONATED(CD) Group

    Control group of FET from donated oocytes (without ERA or PGT-A)

    Other: collect retrospectively data

Interventions

  • Othercollect retrospectively data

    Analyse the incidence of BPL in these populations

05

What researchers measure

Primary outcomes

  1. Biochemical pregnancy loss (BPL)

    when the maternal serum levels of β-hCG are higher than 10 UI/L, but in the transvaginal ultrasound is not possible to appreciate any gestational structure (dichotomous qualitative variable: yes/no). This variable is considered as the fraction between patients whose β-hCG is higher than 10 UI/L, without clinically recognized pregnancy, by number of pregnant patients.

    Time frame: Since 2013 to april 2019

06

Study locations

1 site
  • IVI Vigo
    Vigo, Pontevedra 36203, Spain
07

References and documents

Publications

  • Diaz-Gimeno P, Horcajadas JA, Martinez-Conejero JA, Esteban FJ, Alama P, Pellicer A, Simon C. A genomic diagnostic tool for human endometrial receptivity based on the transcriptomic signature. Fertil Steril. 2011 Jan;95(1):50-60, 60.e1-15. doi: 10.1016/j.fertnstert.2010.04.063. Epub 2010 Jul 8. PubMed 20619403 ↗

Individual participant data

Plan to share: Undecided

08

Registry details

Key details

Study ID
NCT04549909
Lead sponsor
IVI Vigo
Collaborators
Instituto Valenciano de Infertilidad, IVI VALENCIA, IVI Madrid, Fundación IVI
Responsible party
Sponsor
First posted
Sep 16, 2020
Start date
Feb 12, 2019
Primary completion
Jan 15, 2020
Completion
Jan 15, 2020
Last update
Sep 23, 2020

Study contacts

Elkin DR Muñoz, MD
principal investigator · IVI Vigo

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

Not currently enrolling

This study is completed, as verified in Sep 2020. You cannot join it, but the record below documents what was studied.

Follow this study

Get an email when the registry record changes — status, dates, results — or when someone posts here.

Sign in to follow

Discussion

Questions and observations about this study, from anyone following it. Not medical advice, and not a channel to the study team — their contact details are on the registry record.

Sign in to join the discussion. Reading takes no account; posting does. You choose a display name, and a pseudonym is the default.

Nothing here yet. If you are running this trial, taking part in it, or weighing whether to, this is the place to say so.

Start the discussion