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CompletedNCT04475432Saturn-1Updated Dec 21, 2023Results posted

Safety and Efficacy of TP-03 for the Treatment of Demodex Blepharitis

A Phase 2/3 interventional study of TP-03, 0.25% and TP-03 Vehicle in Blepharitis, sponsored by Tarsus Pharmaceuticals, Inc.. Completed at 15 sites in United States. Open to participants aged 18 Years and older. Per ClinicalTrials.gov, last updated 2023-12-21.

Sponsored by Tarsus Pharmaceuticals, Inc. · Phase 2/3, Interventional, and Treatment

Phase
Phase 2/3
Study type
Interventional
Enrollment
421
Allocation
Randomized
Ages
18 Years and older
Sex
All
01

Study summary

The purpose of this study is to compare the safety and efficacy of TP-03, 0.25%, an eyedrop, to its vehicle control for the treatment of blepharitis due to Demodex, a microscopic mite frequently found in human hair follicles. The hypothesis for the study is the proportion of participants cured at Day 43 with treatment by TP-03, 0.25%, is greater than the proportion cured by treatment with its vehicle.

Read the detailed description

This Phase 2b/3 study is a randomized, controlled, multicenter, double-masked, parallel trial to compare the safety and efficacy of TP-03, 0.25%, to vehicle control for the treatment of Demodex blepharitis. The primary objective of the study is to assess the safety and efficacy of TP-03 compared to its vehicle from Day 1 to Day 43 in adult participants with mild to severe Demodex blepharitis. The primary efficacy endpoint will be cure based upon collarettes. Safety will be determined by assessing adverse effects related to the treatment as well as evaluating any changes in visual acuity, intraocular pressure, slit lamp biomicroscopy and dilated ophthalmoscopy findings.

02

Conditions studied

  • Blepharitis

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Keywords

  • Demodex
03

Who can participate

Ages eligible
18 Years and older
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  • Be willing to sign the informed consent and deemed capable of complying with the requirements of the study protocol
  • Meet all of the following criteria in at least one eye: Have more than 10 lashes with collarettes present on the upper lid; have at least mild erythema of the upper eyelid margin; have an average Demodex density, upper and lower eyelids combined, of 1.5 or more mites per lash

Exclusion criteria

Exclusion Criteria:

  • Have used lid hygiene products within 14 days of Screening or unwilling to forego the use of lid hygiene products during the study
  • Have worn contact lenses within 7 days of Screening or be unwilling to forego contact lens wear during the study
  • Have used artificial eyelashes or eyelash extensions within 7 days of Screening or be unwilling to forego their use during the study
  • Pregnancy or lactation
04

Study design

Phase
Phase 2 / Phase 3
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Quadruple (Participant, Care provider, Investigator, Outcomes assessor)
Enrollment
421 participants (actual)

Study arms

  • Experimental
    Active

    TP-03, lotilaner ophthalmic solution, 0.25%, administered topically twice a day for approximately 43 days

    Drug: TP-03, 0.25%

  • Placebo comparator
    Control

    Vehicle of TP-03 ophthalmic solution, administered topically twice a day for approximately 43 days

    Drug: TP-03 Vehicle

Interventions

  • DrugTP-03, 0.25%

    TP-03, lotilaner ophthalmic solution, 0.25%, administered twice a day

    Also known as: Lotilaner ophthalmic solution, 0.25%

  • DrugTP-03 Vehicle

    Vehicle of TP-03 ophthalmic solution, administered twice a day

05

What researchers measure

Primary outcomes

  1. Proportion of Participants Cured Based on a Collarette Score at Day 43.

    The proportion of participants cured where cure is defined as 0-2 lashes with collarettes on the upper eyelid of the analysis eye. The primary outcome analysis is the combined result from 20 analyses each with missing data imputed. Missing data were imputed per the method described in the SAP. The least square mean is computed from the result of the 20 analyses with imputed missing values.

    Time frame: 43 days

Secondary outcomes

  1. The Proportion of Participants With Eradication of Demodex Mites in the Analysis Eye at Day 43

    The proportion of participants with Demodex mites eradicated at Day 43 where eradication is defined as a mite density of 0 mites/lash for the analysis eye. The mite density is obtained by epilating four of more lashes and dividing the number of mites observed under the microscope by the number of lashes. The primary outcome analysis is the combined result from 20 analyses each with missing data imputed. Missing data were imputed per the method described in the SAP. The least square mean is computed from the result of the 20 analyses with imputed missing values

    Time frame: 43 days

  2. Proportion of Participants Cured Based on a Composite Collarette and Erythema Score of 0 in the Analysis Eye at Day 43.

    The proportion of participants cured where cure is defined as a composite of the collarettes, 0-2 lashes with collarettes on the upper eyelid of the analysis eye, and normal lid margin erythema for the upper eyelid of the analysis eye at Day 43. The primary outcome analysis is the combined result from 20 analyses each with missing data imputed. Missing data were imputed per the method described in the SAP. The least square mean is computed from the result of the 20 analyses with imputed missing values.

    Time frame: 43 days

06

Results

Posted Dec 21, 2023

Participant flow

Participant flow — Overall Study
MilestoneActiveControl
Started212209
Completed208203
Not completed46

Outcome measures

PrimaryProportion of Participants Cured Based on a Collarette Score at Day 43.

The proportion of participants cured where cure is defined as 0-2 lashes with collarettes on the upper eyelid of the analysis eye. The primary outcome analysis is the combined result from 20 analyses each with missing data imputed. Missing data were imputed per the method described in the SAP. The least square mean is computed from the result of the 20 analyses with imputed missing values.

Time frame:
43 days
Reported as:
Least squares mean · Proportion cured
Proportion of Participants Cured Based on a Collarette Score at Day 43.
Proportion curedActiveControl
Proportion of Participants Cured Based on a Collarette Score at Day 43.0.439 ± 0.0340.072 ± 0.018
SecondaryThe Proportion of Participants With Eradication of Demodex Mites in the Analysis Eye at Day 43

The proportion of participants with Demodex mites eradicated at Day 43 where eradication is defined as a mite density of 0 mites/lash for the analysis eye. The mite density is obtained by epilating four of more lashes and dividing the number of mites observed under the microscope by the number of lashes. The primary outcome analysis is the combined result from 20 analyses each with missing data imputed. Missing data were imputed per the method described in the SAP. The least square mean is computed from the result of the 20 analyses with imputed missing values

Time frame:
43 days
Reported as:
Least squares mean · Proportion eradicated
The Proportion of Participants With Eradication of Demodex Mites in the Analysis Eye at Day 43
Proportion eradicatedActiveControl
The Proportion of Participants With Eradication of Demodex Mites in the Analysis Eye at Day 430.677 ± 0.0320.173 ± 0.026
SecondaryProportion of Participants Cured Based on a Composite Collarette and Erythema Score of 0 in the Analysis Eye at Day 43.

The proportion of participants cured where cure is defined as a composite of the collarettes, 0-2 lashes with collarettes on the upper eyelid of the analysis eye, and normal lid margin erythema for the upper eyelid of the analysis eye at Day 43. The primary outcome analysis is the combined result from 20 analyses each with missing data imputed. Missing data were imputed per the method described in the SAP. The least square mean is computed from the result of the 20 analyses with imputed missing values.

Time frame:
43 days
Reported as:
Least squares mean · Proportion cured
Proportion of Participants Cured Based on a Composite Collarette and Erythema Score of 0 in the Analysis Eye at Day 43.
Proportion curedActiveControl
Proportion of Participants Cured Based on a Composite Collarette and Erythema Score of 0 in the Analysis Eye at Day 43.0.139 ± 0.0240.01 ± 0.007

Adverse events

Collected over 57 days. Non-serious events are listed at a 1% frequency threshold.

Adverse event summary by group
GroupDeathsSeriousOther
Active0/212 (0%)3/212 (1.4%)37/212 (17.5%)
Control0/209 (0%)1/209 (0.5%)39/209 (18.7%)
Most frequent serious events
Most frequent serious events
EventActiveControl
Vascular access site pseudoaneurysmInjury, poisoning and procedural complications0/2121/209
COVID-19Infections and infestations1/2120/209
PneumoniaInfections and infestations1/2120/209
HaematuriaRenal and urinary disorders1/2120/209
Most frequent other events
Most frequent other events
EventActiveControl
Instillation site painGeneral disorders25/21216/209
Instillation site pruritusGeneral disorders3/2127/209
Visual acuity reducedEye disorders6/2126/209
Eye painEye disorders3/2123/209
ChalazionEye disorders1/2123/209
Eye dischargeEye disorders3/2122/209
Vision blurredEye disorders2/2122/209
Eye pruritusEye disorders1/2122/209
Swelling of eyelidEye disorders1/2122/209

Baseline characteristics

Age, Categorical
Age, Categorical(Participants)ActiveControlTotal
<=18 years000
Between 18 and 65 years8665151
>=65 years126144270
Age, Continuous
Age, Continuous(years)ActiveControlTotal
Mean66.1 ± 12.0967.8 ± 12.6367.0 ± 12.37
Sex: Female, Male
Sex: Female, Male(Participants)ActiveControlTotal
Female123117240
Male8992181
Ethnicity (NIH/OMB)
Ethnicity (NIH/OMB)(Participants)ActiveControlTotal
Hispanic or Latino141125
Not Hispanic or Latino198198396
Unknown or Not Reported000
Race (NIH/OMB)
Race (NIH/OMB)(Participants)ActiveControlTotal
American Indian or Alaska Native112
Asian325
Native Hawaiian or Other Pacific Islander000
Black or African American111627
White195187382
More than one race235
Unknown or Not Reported000
Region of Enrollment
Region of Enrollment(participants)ActiveControlTotal
United States212209421
07

Study locations

15 sites
  • Cornea and Cataract Consultants of Arizona
    Phoenix, Arizona 85032, United States
  • Eye Research Foundation
    Newport Beach, California 92663, United States
  • Visionary Eye Institute
    Newport Beach, California 92663, United States
  • Vision Institute
    Colorado Springs, Colorado 80907, United States
  • Midwest Cornea Associates, LLC
    Carmel, Indiana 46290, United States
  • Washburn Research LLC
    Indianapolis, Indiana 46240, United States
  • The Eye Care Institute
    Louisville, Kentucky 40206, United States
  • Andover Eye Associates
    Andover, Massachusetts 01810, United States
  • Ophthalmology Associates
    Saint Louis, Missouri 63131, United States
  • Oculus Research, Inc at EyecareCenter
    Raleigh, North Carolina 27603, United States
  • Vita Eye Clinic
    Shelby, North Carolina 28150, United States
  • Scott & Christie and Associates, PC
    Cranberry Township, Pennsylvania 16066, United States
  • Total Eye Care, P.A.
    Memphis, Tennessee 38119, United States
  • Alpine Research Organization
    Layton, Utah 84041, United States
  • Piedmont Eye Center
    Lynchburg, Virginia 24502, United States
08

References and documents

Publications

  • Yeu E, Wirta DL, Karpecki P, Baba SN, Holdbrook M; Saturn I Study Group. Lotilaner Ophthalmic Solution, 0.25%, for the Treatment of Demodex Blepharitis: Results of a Prospective, Randomized, Vehicle-Controlled, Double-Masked, Pivotal Trial (Saturn-1). Cornea. 2023 Apr 1;42(4):435-443. doi: 10.1097/ICO.0000000000003097. Epub 2022 Aug 10. PubMed 35965392 ↗

Study documents

  • Study protocol · Jan 25, 2021
  • Statistical analysis plan · May 26, 2021

Documents are hosted by the registry — open the source record to download them.

Individual participant data

Plan to share: No

09

Registry details

Key details

Study ID
NCT04475432
Lead sponsor
Tarsus Pharmaceuticals, Inc.
Responsible party
Sponsor
First posted
Jul 17, 2020
Start date
Sep 9, 2020
Primary completion
May 4, 2021
Completion
May 4, 2021
Results posted
Dec 21, 2023
Last update
Dec 21, 2023

Study contacts

David Wirta, MD
principal investigator · Eye Research Foundation

Oversight

Data monitoring committee
No
FDA-regulated drug
Yes
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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