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CompletedNCT04248049Updated Jan 30, 2020

Platelet Rich Fibrin on Root Coverage of Gingival Recessions

An interventional study of Platelet rich fibrin in Gingival Recession, sponsored by Universidad Antonio Nariño. Completed at 1 site in Colombia. Open to participants aged 18 Years to 60 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2020-01-30.

Sponsored by Universidad Antonio Nariño · Not applicable, Interventional, and Treatment

Phase
Not applicable
Study type
Interventional
Enrollment
15
Allocation
Randomized
Ages
18 Years to 60 Years
Sex
All
01

Study summary

Several surgical techniques and various adjunctive agents have been used to covered Gingival Recession (GR) and promote clinical outcomes. The treatment of the GR more used (gold standard) is the root coverage with surgical procedures and connective tissue graft, but it has limitations due to the conditions of the donor graft. Is necessary develop other biomaterials for these cases.

The platelet rich fibrin is a autologous biomaterial that has gained tremendous momentum having been utilized for a variety of dental and medical procedures including periodontal surgeries. However, results remain contradictory and mainly focus on the hard and soft tissue healing, aesthetics and postoperative discomfort.

The objective of this study was to evaluate the clinical effects of coronal advanced flap (CAF) and CAF + Platelet Rich Fibrin (PRF) on Root coverage (RC) over a period of 24 month.

02

Conditions studied

  • Gingival Recession

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Keywords

  • platelet-rich fibrin
  • platelet concentrates
  • periodontal diseases
03

Who can participate

Ages eligible
18 Years to 60 Years
Sexes eligible
All
Accepts healthy volunteers
Yes

Inclusion criteria

  • Participant is willing and able to give informed consent for participation in the study
  • Healthy males and females of age 18-60 years old
  • Multiple bilateral (maxillary or mandibular) gingival recessions (Miller class І or II)
  • Good oral hygiene (O´Leary index ≤ 15%)

Exclusion criteria

Exclusion Criteria:

  • Active periodontal disease
  • Periodontal surgeries in the last three years
  • Pregnancy or lactation
  • Smokers or alcoholics
  • Platelet dysfunction syndrome or thrombocytopenia
  • Coagulation defects
  • Uncontrolled diabetes
  • Medical contraindications to elective oral surgery procedures
  • Severe immunodeficiencies
  • Oncological history
  • Prosthetic restorations adjacent to gingival recessions
  • Mobility II or III
  • Occlusal contacts excessive
  • Root caries
  • Buccal restaurations Class V
04

Study design

Phase
Not applicable
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Quadruple (Participant, Care provider, Investigator, Outcomes assessor)
Enrollment
15 participants (actual)

Study arms

  • Experimental
    Experimental side

    Roots covered with coronally advanced flap (CAF) and platelet rich fibrin (PRF) (CAF+PRF)

    Other: Platelet rich fibrin

  • Active comparator
    Control side

    Roots covered with coronally advanced flap (CAR)

    Other: Platelet rich fibrin

Interventions

  • OtherPlatelet rich fibrin

    The roots of patients with diagnosis of gingival recessions will be covered with coronally advanced flap (CAF) and platelet rich fibrin (PRF) (CAF+PRF)

05

What researchers measure

Primary outcomes

  1. Change of percentage of root coverage (PRC) from baseline at 12 and 24 months

    Complete root coverage after surgical correction measured in percentage by using periodontal probe. PRC= Preoperative recession depth - Postoperative recession depth X 100 % Preoperative recession depth

    Time frame: baseline, 1, 3 , 6, 9, 12 and 24 months

Secondary outcomes

  1. Vertical gingival recession (VGR)

    It is measuring the distance from the cemento-enamel junction (CEJ) to the margin of the gingiva at the midbuccal point of the teeth (measured using a periodontal probe in millimeters).

    Time frame: baseline, 1, 3 , 6, 9, 12 and 24 months

  2. Horizontal gingival recession (HGR)

    Measured horizontally between two borders of the recession. (measured using a periodontal probe in millimeters).

    Time frame: baseline, 1, 3 , 6, 9, 12 and 24 months

  3. Probing depth (PD)

    It is measuring the distance from the gingival margin to the base of the pocket using periodontal probe. The probe will be inserted parallel to the long axis of the tooth using light force. (measured using a periodontal probe in millimeters).

    Time frame: baseline, 1, 3 , 6, 9, 12 and 24 months

  4. Clinical attachment level(CAL)

    Two measurements will be used to calculate the CAL: the probing depth and the distance from the gingival margin to the CEJ.

    Time frame: baseline, 1, 3 , 6, 9, 12 and 24 months

  5. Gingival tissue thickness

    The gingiva mid-buccally in the attached gingiva will be penetrated with an endodontic reamer with a silicon disc stop. The measure of penetration will be measured with dentimeter (mm).

    Time frame: baseline, 1, 3 , 6, 9, 12 and 24 months

  6. Keratinized tissue height (KTH)

    To measure the tissue thickness, a guiding device will be made to standardize the region to be measured and then, at a specific point, the mucosa will be punctured with an endodontic spacer and the thickness marked with a rubber marker. Then, using a digital caliper, the thickness will be measured.

    Time frame: baseline, 1, 3 , 6, 9, 12 and 24 months

  7. Gingival bleeding index (IS)

    Evaluation of the presence and absence of bleeding in the gingival margin through the gingival sulcus. The index is calculated after penetration of the sulcus by the periodontal probe, bleeding surfaces are noted and then counted. The index is calculated by dividing the number of surfaces that bleed by the total number of teeth and then multiplying by 100 to reach the percentage of bleeding index.

    Time frame: baseline, 1, 3 , 6, 9, 12 and 24 months

  8. Visual analog scale (VAS)

    Visual analog scale

    Time frame: baseline, 1, 3 , 6, 9, 12 and 24 months

  9. Evaluation of oral health impact on quality of life, using the Oral Health Impact Profile (OHIP-14) questionnaire.

    Evaluate the impact of oral health on patient's quality of life before and 1 year after implant installation. This evaluation will be carried out through the application of a questionnaire with 14 questions (Oral Health Impact Profile: OHIP-14) that assess the patient's perception regarding the impact of oral conditions on their well-being

    Time frame: baseline, 1, 3 , 6, 9, 12 and 24 months

06

Study locations

1 site
  • Universidad Antonio Nariño
    Bogotá, 110231, Colombia
07

References and documents

Publications

  • Li R, Liu Y, Xu T, Zhao H, Hou J, Wu Y, Zhang D. The Additional Effect of Autologous Platelet Concentrates to Coronally Advanced Flap in the Treatment of Gingival Recessions: A Systematic Review and Meta-Analysis. Biomed Res Int. 2019 Jul 25;2019:2587245. doi: 10.1155/2019/2587245. eCollection 2019. PubMed 31428630 ↗
  • Debnath K, Chatterjee A. Evaluation of periosteum eversion and coronally advanced flap techniques in the treatment of isolated Miller's Class I/II gingival recession: A comparative clinical study. J Indian Soc Periodontol. 2018 Mar-Apr;22(2):140-149. doi: 10.4103/jisp.jisp_5_18. PubMed 29769769 ↗
  • Mufti S, Dadawala SM, Patel P, Shah M, Dave DH. Comparative Evaluation of Platelet-Rich Fibrin with Connective Tissue Grafts in the Treatment of Miller's Class I Gingival Recessions. Contemp Clin Dent. 2017 Oct-Dec;8(4):531-537. doi: 10.4103/ccd.ccd_325_17. PubMed 29326502 ↗
  • Miron RJ, Zucchelli G, Pikos MA, Salama M, Lee S, Guillemette V, Fujioka-Kobayashi M, Bishara M, Zhang Y, Wang HL, Chandad F, Nacopoulos C, Simonpieri A, Aalam AA, Felice P, Sammartino G, Ghanaati S, Hernandez MA, Choukroun J. Use of platelet-rich fibrin in regenerative dentistry: a systematic review. Clin Oral Investig. 2017 Jul;21(6):1913-1927. doi: 10.1007/s00784-017-2133-z. Epub 2017 May 27. PubMed 28551729 ↗

Individual participant data

Plan to share: No

08

Registry details

Key details

Study ID
NCT04248049
Lead sponsor
Universidad Antonio Nariño
Responsible party
Sponsor
First posted
Jan 30, 2020
Start date
Jan 1, 2018
Primary completion
Aug 30, 2019
Completion
Nov 30, 2019
Last update
Jan 30, 2020

Oversight

Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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