An observational study in Dental Erosion, sponsored by University of Göttingen. Completed at 1 site in Germany. Open to participants aged 20 Years to 50 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2026-01-27.
Sponsored by University of Göttingen · Observational
The aim of this study is to investigate whether smoking is associated with changes in salivary composition and/or predisposition to erosion.
Healthy volunteers are observationally wearing an intraoral device with both bovine tooth specimens (enamel and dentin) and resin specimens twice for two hours each. Afterwards, specimens are eroded extraorally and calcium release into the acid is measured.
Total protein concentration and protein composition of the salivary pellicles on the resin samples are measured. Additionally, salivary parameters (unstimulated and stimulated saliva flow rate, pH, buffer capacity, total protein content and protein composition as well as concentration of inorganic calcium, phosphate, and fluoride) are measured.
Exploratory study
Exclusion Criteria:
Volunteers smoking at least 10 cigarettes per day
Other: Use of fluoridated toothpaste · Other: Wearing of an intraoral device with bovine tooth samples · Other: Wearing of an intraoral device with resin samples
Non-smoking volunteers
Other: Use of fluoridated toothpaste · Other: Wearing of an intraoral device with bovine tooth samples · Other: Wearing of an intraoral device with resin samples
Use of fluoridated toothpaste
Wearing of an intraoral device with bovine tooth samples
Wearing of an intraoral device with resin samples
Calcium release from bovine enamel and dentin specimens by extraoral erosion (nmol/Square Millimeter).
Time frame: Immediately after the intraoral device has been worn once for two hours.
Determination of unstimulated and stimulated saliva flow rate (mL/min).
Time frame: Saliva samples are collected for 5 min each on three visits.
Determination of saliva pH (pH).
Time frame: Saliva samples are collected for 5 min each on three visits.
Determination of salivary buffer capacity (pH).
Time frame: Saliva samples are collected for 5 min each on three visits.
Determination of total protein content (mg/L) and protein composition (qualitatively) in saliva.
Time frame: Saliva samples are collected for 5 min each on three visits.
Determination of inorganic calcium (mmol/L), phosphate (mmol/L) and fluoride (µmol/L) in saliva.
Time frame: Saliva samples are collected for 5 min each on three visits.
Determination of total protein concentration (ng/Square Millimeter) and protein composition (qualitatively) within the salivary pellicles.
Time frame: Immediately after the intraoral device with resin samples has been worn once for two hours.
Plan to share: No
This study is completed, as verified in Jan 2026. You cannot join it, but the record below documents what was studied.
Get an email when the registry record changes — status, dates, results — or when someone posts here.
Sign in to followQuestions and observations about this study, from anyone following it. Not medical advice, and not a channel to the study team — their contact details are on the registry record.
Sign in to join the discussion. Reading takes no account; posting does. You choose a display name, and a pseudonym is the default.
Nothing here yet. If you are running this trial, taking part in it, or weighing whether to, this is the place to say so.
University of Göttingen