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CompletedNCT03709121Updated Feb 24, 2025Results posted

A Methodology Development Clinical Trial of Reproxalap in Subjects With Seasonal Allergic Conjunctivitis Using the Environmental Exposure Chamber

A Phase 1/2 interventional study of Reproxalap Ophthalmic Solution (0.25%) and Reproxalap Ophthalmic Solution (0.5%) in Conjunctivitis, Allergic, sponsored by Aldeyra Therapeutics, Inc.. Completed at 1 site in Canada. Open to participants aged 18 Years and older. Per ClinicalTrials.gov, last updated 2025-02-24.

Sponsored by Aldeyra Therapeutics, Inc. · Phase 1/2, Interventional, and Treatment

Phase
Phase 1/2
Study type
Interventional
Enrollment
70
Allocation
Randomized
Ages
18 Years and older
Sex
All
01

Study summary

An Exploratory Clinical Trial Evaluating Reproxalap Ophthalmic Solutions (0.25% and 0.5%) in Subjects with Seasonal Allergic Conjunctivitis Using the Environmental Exposure Chamber (EEC)

02

Conditions studied

  • Conjunctivitis, Allergic

Keywords

  • reproxalap
  • ADX-102
  • allergic conjunctivitis
03

Who can participate

Ages eligible
18 Years and older
Sexes eligible
All
Accepts healthy volunteers
No

Inclusion criteria

  • be at least 18 years of age of either gender and any race
  • have at least a two-year history of moderate-to-severe ragweed-induced allergic conjunctivitis based on principal investigator's judgement
  • have a positive skin prick test to ragweed pollen within the past year of screening

Exclusion criteria

Exclusion Criteria:

  • known contraindication or hypersensitivities to any components of the investigational product medication or components
  • history of uveitis, blepharitis, dry eye syndrome, herpes simplex keratitis, or herpes zoster keratitis;
  • presence of any ocular infection (bacterial, viral, or fungal) or active ocular inflammation (e.g., follicular conjunctivitis, allergic conjunctivitis) within 14 days prior to screening
  • presence of any chronic ocular degenerative condition or ocular inflammation that, in the opinion of the investigator, is likely to worsen over the course of the clinical trial;
  • presence of any chronic ocular degenerative condition or ocular inflammation that, in the opinion of the investigator, is likely to worsen over the course of the clinical trial
  • diagnosis of moderate-to-severe pinguecula or pterygium (particularly if it results in chronic erythema), Stevens-Johnson Syndrome, ocular cicatricial pemphigoid, mucous membrane pemphigoid, significant conjunctival scarring, chemical burn, herpetic or neurotrophic keratitis, Cryopyrin Associated Periodic Syndrome (CAPS), or keratoconus
  • woman of childbearing potential who is pregnant or nursing
04

Study design

Phase
Phase 1 / Phase 2
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Crossover assignment
Masking
Quadruple (Participant, Care provider, Investigator, Outcomes assessor)
Enrollment
70 participants (actual)

Study arms

  • Experimental
    Reproxalap Ophthalmic Solution (0.25%)

    Drug: Reproxalap Ophthalmic Solution (0.25%)

  • Experimental
    Reproxalap Ophthalmic Solution (0.5%)

    Drug: Reproxalap Ophthalmic Solution (0.5%)

  • Placebo comparator
    Vehicle Ophthalmic Solution

    Drug: Vehicle Ophthalmic Solution

Interventions

  • DrugReproxalap Ophthalmic Solution (0.25%)

    Reproxalap Ophthalmic Solution (0.25%) dosed twice.

  • DrugReproxalap Ophthalmic Solution (0.5%)

    Reproxalap Ophthalmic Solution (0.5%) dosed twice.

  • DrugVehicle Ophthalmic Solution

    Vehicle Ophthalmic Solution dosed twice.

05

What researchers measure

Primary outcomes

  1. Subject-Reported Ocular Itching Score

    Change from baseline comparison of reproxalap to vehicle for subject-reported ocular itching score from 0 to 212 minutes in the allergen chamber using a 0 -100 millimeter visual analogue scale (0 = none, 100 = severe) was assessed. The least squares mean (standard error) was derived from mixed model repeated measures for change from baseline included itching score as a dependent variable, treatment, period, and interaction of treatment and period as fixed effects.

    Time frame: Efficacy was assessed from 0 to 212 minutes in the allergen chamber.

Secondary outcomes

  1. Subject-Reported Ocular Tearing Score

    Change from baseline comparison of reproxalap to vehicle for subject-reported ocular tearing score from 0 to 212 minutes in the allergen chamber using a 4-point scale (0 = none, 3 = severe) was assessed. The least squares mean (standard error) was derived from mixed model repeated measures for change from baseline included itching score as a dependent variable, treatment, period, and interaction of treatment and period as fixed effects.

    Time frame: Efficacy was assessed from 0 to 212 minutes in the allergen chamber.

  2. Investigator-Assessed Conjunctival Redness Score

    Change from baseline comparison of reproxalap to vehicle for investigator-assessed conjunctival redness from 0 to 210 minutes in the allergen chamber using a 9-point scale with half unit increments (0 = normal, 4 = prominent) was assessed. The least squares mean (standard error) was derived from mixed model repeated measures for change from baseline included itching score as a dependent variable, treatment, period, and interaction of treatment and period as fixed effects.

    Time frame: Efficacy was assessed from 0 to 210 minutes in the allergen chamber.

06

Results

Posted Feb 24, 2025

Participant flow

Participant flow — Overall Study
MilestoneReproxalap (0.25%), Then Reproxalap (0.5%), Then VehicleReproxalap (0.5%), Then Vehicle, Then Reproxalap (0.25%)Vehicle, Then Reproxalap (0.25%), Then Reproxalap (0.5%)
Started222523
Completed222222
Not completed031

Outcome measures

PrimarySubject-Reported Ocular Itching Score

Change from baseline comparison of reproxalap to vehicle for subject-reported ocular itching score from 0 to 212 minutes in the allergen chamber using a 0 -100 millimeter visual analogue scale (0 = none, 100 = severe) was assessed. The least squares mean (standard error) was derived from mixed model repeated measures for change from baseline included itching score as a dependent variable, treatment, period, and interaction of treatment and period as fixed effects.

Time frame:
Efficacy was assessed from 0 to 212 minutes in the allergen chamber.
Reported as:
Least squares mean · units on a scale
Subject-Reported Ocular Itching Score
units on a scaleReproxalap (0.25%)Reproxalap (0.5%)Vehicle
Subject-Reported Ocular Itching Score16.410 ± 1.619516.964 ± 1.615022.310 ± 1.6188
SecondarySubject-Reported Ocular Tearing Score

Change from baseline comparison of reproxalap to vehicle for subject-reported ocular tearing score from 0 to 212 minutes in the allergen chamber using a 4-point scale (0 = none, 3 = severe) was assessed. The least squares mean (standard error) was derived from mixed model repeated measures for change from baseline included itching score as a dependent variable, treatment, period, and interaction of treatment and period as fixed effects.

Time frame:
Efficacy was assessed from 0 to 212 minutes in the allergen chamber.
Reported as:
Least squares mean · units on a scale
Subject-Reported Ocular Tearing Score
units on a scaleReproxalap (0.25%)Reproxalap (0.5%)Vehicle
Subject-Reported Ocular Tearing Score0.8777 ± 0.06150.8915 ± 0.06131.0507 ± 0.0614
SecondaryInvestigator-Assessed Conjunctival Redness Score

Change from baseline comparison of reproxalap to vehicle for investigator-assessed conjunctival redness from 0 to 210 minutes in the allergen chamber using a 9-point scale with half unit increments (0 = normal, 4 = prominent) was assessed. The least squares mean (standard error) was derived from mixed model repeated measures for change from baseline included itching score as a dependent variable, treatment, period, and interaction of treatment and period as fixed effects.

Time frame:
Efficacy was assessed from 0 to 210 minutes in the allergen chamber.
Reported as:
Least squares mean · units on a scale
Investigator-Assessed Conjunctival Redness Score
units on a scaleReproxalap (0.25%)Reproxalap Ophthalmic Solution (0.5%)Vehicle
Investigator-Assessed Conjunctival Redness Score0.5004 ± 0.04580.5462 ± 0.04570.6497 ± 0.0458

Adverse events

Collected over The period of time over which adverse events were collected for each subject in the clinical trial was approximately fourteen weeks.. Non-serious events are listed at a 3% frequency threshold.

Adverse event summary by group
GroupDeathsSeriousOther
Reproxalap (0.25%)0/67 (0%)0/67 (0%)49/67 (73.1%)
Reproxalap (0.5%)0/69 (0%)0/69 (0%)56/69 (81.2%)
Vehicle Ophthalmic Solution0/67 (0%)0/67 (0%)5/67 (7.5%)
Most frequent other events
Most frequent other events
EventReproxalap (0.25%)Reproxalap (0.5%)Vehicle Ophthalmic Solution
General disorders and administration site conditionsGeneral disorders49/6756/695/67

Baseline characteristics

Age, Customized
Age, Customized(years)Reproxalap (0.25%), Then Reproxalap (0.5%), Then VehicleReproxalap (0.5%), Then Vehicle, Then Reproxalap (0.25%)Vehicle, Then Reproxalap (0.25%), Then Reproxalap (0.5%)Total
Mean45.50 (27 to 66)48.84 (28 to 67)46.17 (30 to 68)46.9 (27 to 68)
Sex: Female, Male
Sex: Female, Male(Participants)Reproxalap (0.25%), Then Reproxalap (0.5%), Then VehicleReproxalap (0.5%), Then Vehicle, Then Reproxalap (0.25%)Vehicle, Then Reproxalap (0.25%), Then Reproxalap (0.5%)Total
Female12161442
Male109928
Ethnicity (NIH/OMB)
Ethnicity (NIH/OMB)(Participants)Reproxalap (0.25%), Then Reproxalap (0.5%), Then VehicleReproxalap (0.5%), Then Vehicle, Then Reproxalap (0.25%)Vehicle, Then Reproxalap (0.25%), Then Reproxalap (0.5%)Total
Hispanic or Latino2259
Not Hispanic or Latino20231861
Unknown or Not Reported0000
Race/Ethnicity, Customized
Race/Ethnicity, Customized(Participants)Reproxalap (0.25%), Then Reproxalap (0.5%), Then VehicleReproxalap (0.5%), Then Vehicle, Then Reproxalap (0.25%)Vehicle, Then Reproxalap (0.25%), Then Reproxalap (0.5%)Total
White15171345
American Indian or Alaska Native0000
Black or African American45514
Native Hawaiian / Other Pacific Islander0000
Asian1359
Other - Mixed2002
Region of Enrollment
Region of Enrollment(participants)Reproxalap (0.25%), Then Reproxalap (0.5%), Then VehicleReproxalap (0.5%), Then Vehicle, Then Reproxalap (0.25%)Vehicle, Then Reproxalap (0.25%), Then Reproxalap (0.5%)Total
Canada22252370
07

Study locations

1 site
  • Inflamax Research Limited
    Mississauga, Ontario L4W 1V7/L4W 1N2, Canada
08

References and documents

Study documents

  • Study protocol · Nov 22, 2018
  • Statistical analysis plan · Mar 3, 2019

Documents are hosted by the registry — open the source record to download them.

Individual participant data

Plan to share: No

09

Registry details

Key details

Study ID
NCT03709121
Lead sponsor
Aldeyra Therapeutics, Inc.
Responsible party
Sponsor
First posted
Oct 17, 2018
Start date
Oct 2, 2018
Primary completion
Mar 20, 2019
Completion
Mar 20, 2019
Results posted
Feb 24, 2025
Last update
Feb 24, 2025

Oversight

Data monitoring committee
No
FDA-regulated drug
Yes
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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