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Status unknownNCT03437759MSCsUpdated Apr 6, 2021

MSC-Exos Promote Healing of MHs

An Early Phase 1 interventional study of exosomes derived from mesenchymal stem cells (MSC-Exo) in Macular Holes, sponsored by Tianjin Medical University. Status unknown at 1 site in China. Open to participants aged Up to 80 Years, including healthy volunteers. Per ClinicalTrials.gov, last updated 2021-04-06.

Sponsored by Tianjin Medical University · Early Phase 1, Interventional, and Treatment

The sponsor has not verified this record recently (last verified Apr 2021), so the status shown — last known as Active, not recruiting — may be out of date.
Phase
Early Phase 1
Study type
Interventional
Enrollment
44
Allocation
Randomized
Ages
Up to 80 Years
Sex
All
01

Study summary

Purpose: To assess the safety and efficacy of mesenchymal stem cells (MSCs) and MSC-derived exosomes (MSC-Exos) for promoting healing of large and refractory macular holes (MHs).

Hypothesis: MSC and MSC-Exo therapy may promote functional and anatomic recovery from MH. MSC-Exo therapy may be a useful and safe method for improving visual outcomes of surgery for refractory MHs.

Read the detailed description

Based on the purpose and hypothesis,the participants with large and longstanding idiopathic MHs underwent vitrectomy, internal limiting membrane peeling, MSC or MSC-Exo intravitreal injection, and heavy silicon oil, air, 20% SF6, or 14% C3F8 tamponade. MSCs were isolated from human umbilical cord, and MSC-Exos were isolated from supernatants of MSCs via sequential ultracentrifugation. At the time of study enrollment, as well as physical examinations, best-corrected visual acuity (BCVA) and intraocular pressure will be measured and fundoscopy be performed. All diagnoses of MH are going to confirmed via spectral-domain optical coherence tomography (OCT), and the minimum linear diameter (MLD) of each MH will be measured parallel to the retinal pigment epithelium.The participants are going to be followed up for at least 6 months via BCVA measurement, fundoscopy, OCT, and physical examinations.

02

Conditions studied

  • Macular Holes

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Keywords

  • macular hole (MH)
  • exosomes
  • mesenchymal stem cells
  • pars plana vitrectomy
03

Who can participate

Ages eligible
Up to 80 Years
Sexes eligible
All
Accepts healthy volunteers
Yes

Inclusion criteria

1.Clinical diagnosis of idiopathic macular hole whose diameter is greater than 400 microns

Exclusion criteria

Exclusion Criteria:

  1. Only one functional eye
  2. In other clinical trials.
  3. Other diseases which can affect visual acuity, such as cataract, diabetic retinopathy, glaucoma, corneal diseases, etc.
  4. Eye had undergone vitrectomy or scleral buckling, cataract surgery, Nd:YAG laser less than one month ago.
  5. Contralateral eye has poor prognosis than the study eye.
  6. Idiopathic or autoimmune uveitis history.
  7. Aphakic eye
  8. Physical condition is poor that can not keep prone position.
  9. Secondary macular lesions
  10. The equivalent spherical diopter of the study eye before any refractive correction or cataract surgery, which is greater than 6.0d or above 26mm of the ocular axis of the study eye.
  11. Intraocular pressure is higher than 25mmHg
  12. Within ocular inflammation, such as eye blepharitis, scleritis, keratitis and conjunctivitis.
  13. Systemic condition is poor, such as the poor control of diabetes and hypertension, myocardial infarction, cerebrovascular accident, renal failure and so on,and the researchers assessed those who are unable to complete the trail.
04

Study design

Phase
Early Phase 1
Primary purpose
Treatment
Allocation
Randomized
Intervention model
Parallel assignment
Masking
Single (Participant)
Enrollment
44 participants (estimated)

Study arms

  • Experimental
    Experimental group

    Our intervention is to add treatment of exosomes derived from mesenchymal stem cells (MSC-Exo) after pars plana vitrectomy(PPV) and ILM peeling.

    Biological: exosomes derived from mesenchymal stem cells (MSC-Exo)

  • No intervention
    Control group

    Control group that receives treatment of only pars plana vitrectomy(PPV) and ILM peeling.

Interventions

  • Biologicalexosomes derived from mesenchymal stem cells (MSC-Exo)

    After air-liquid exchange, 50μg or 20μg MSC-Exo in 10μl PBS was dripped into vitreous cavity around MH, leaving 20% SF6 or air as tamponade .

05

What researchers measure

Primary outcomes

  1. Macular holes closure

    Minimum linear diameter (MLD) of the hole measured by OCT

    Time frame: baseline to 24 weeks post-surgery

Secondary outcomes

  1. Best corrected visual acuity(BCVA)

    BCVA using a Landolt C acuity chart method

    Time frame: baseline to 24 weeks post-surgery

06

Study locations

1 site
  • Tianjin Medical University Hospital
    Tianjin, China
07

References and documents

Publications

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  • Jiang Y, Zhang Y, Zhang L, Wang M, Zhang X, Li X. Therapeutic effect of bone marrow mesenchymal stem cells on laser-induced retinal injury in mice. Int J Mol Sci. 2014 May 27;15(6):9372-85. doi: 10.3390/ijms15069372. PubMed 24871366 ↗
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  • Li G, Yuan L, Ren X, Nian H, Zhang L, Han ZC, Li X, Zhang X. The effect of mesenchymal stem cells on dynamic changes of T cell subsets in experimental autoimmune uveoretinitis. Clin Exp Immunol. 2013 Jul;173(1):28-37. doi: 10.1111/cei.12080. PubMed 23607419 ↗
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Individual participant data

Plan to share: No

08

Registry details

Key details

Study ID
NCT03437759
Lead sponsor
Tianjin Medical University
Responsible party
Xiaomin Zhang (Principal Investigator, Tianjin Medical University Eye Hospital) — Principal investigator
First posted
Feb 19, 2018
Start date
Mar 1, 2017
Primary completion
May 30, 2018
Completion
Dec 30, 2021 (estimated)
Last update
Apr 6, 2021

Study contacts

Rong X Li, MD,PhD
principal investigator · Tianjin Medical University Eye Hospital

Oversight

Data monitoring committee
Yes
FDA-regulated drug
No
FDA-regulated device
No
View the source record on ClinicalTrials.gov ↗

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